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Full-Text Articles in Life Sciences
Study Of Zyomogen Granule Movement Along Actin Filaments Using A Single Beam Optical Trap, Justin James Raupp
Study Of Zyomogen Granule Movement Along Actin Filaments Using A Single Beam Optical Trap, Justin James Raupp
Wayne State University Dissertations
Zymogen granules are enzymatic vesicles in the pancreas. The surface of these zymogen granules (ZGs) has several different kinds of myosin molecules, such as myosin 1c, 6, 5c, and 7b. These molecular motors may contribute to ZG transportation in cells. To understand the molecular motors involved in the vesicle trafficking, we observed the in vitro motility of purified ZGs from rat pancreas and examined the stepping behavior and force that is generated using a single beam optical trap. To be involved in trafficking, molecular motors have certain characteristics, a high duty ratio and the ability to move continuously along actin …
Abelson Tyrosine Kinase Links Axon Guidance Receptors To Cytoskeletal Dynamics Through Protein-Protein Interactions Mediated By Its C-Terminal Domain, Han Sian Joshua Cheong
Abelson Tyrosine Kinase Links Axon Guidance Receptors To Cytoskeletal Dynamics Through Protein-Protein Interactions Mediated By Its C-Terminal Domain, Han Sian Joshua Cheong
Wayne State University Dissertations
Abelson tyrosine kinase (Abl) is a key player in axon guidance, where it functions to link a multitude of axon guidance receptors to actin cytoskeletal dynamics. The C-terminal domain (CTD) of Abl is instrumental to Abl function, but its role is poorly explored due to a lack of conserved sequence elements. Here, we use a systematic approach to identify regions of the CTD important for Abl function, and define two regions (the first and third quarters, 1Q and 3Q) important for Abl function. We demonstrate that both these regions are likely intrinsically disordered, and function in scaffolding to recruit Abl …