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Articles 1 - 13 of 13
Full-Text Articles in Life Sciences
Elucidating A Role For The Cytoplasmic Domain In The Mycobacterium Tuberculosis Mechanosensitive Channel Of Large Conductance, Nadia Herrera, Grigory Maksaev, Elizabeth S. Haswell, Douglas Rees
Elucidating A Role For The Cytoplasmic Domain In The Mycobacterium Tuberculosis Mechanosensitive Channel Of Large Conductance, Nadia Herrera, Grigory Maksaev, Elizabeth S. Haswell, Douglas Rees
Biology Faculty Publications & Presentations
Microbial survival in dynamic environments requires the ability to successfully respond to abrupt changes in osmolarity. The mechanosensitive channel of large conductance (MscL) is a ubiquitous channel that facilitates the survival of bacteria and archaea under severe osmotic downshock conditions by relieving excess turgor pressure in response to increased membrane tension. A prominent structural feature of MscL, the cytoplasmic C-terminal domain, has been suggested to infuence channel assembly and function. In this report, we describe the X-ray crystal structure and electrophysiological properties of a C-terminal domain truncation of the Mycobacterium tuberculosis MscL (MtMscLΔC). A crystal structure of MtMscLΔC solubilized in …
Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell
Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Mechanosensitive (MS) ion channels provide a universal mechanism for sensing and responding to increased membrane tension. MscS‐like (MSL) 10 is a relatively well‐studied MS ion channel from Arabidopsis thaliana that is implicated in cell death signaling. The relationship between the amino acid sequence of MSL10 and its conductance, gating tension, and opening and closing kinetics remains unstudied. Here, we identify several nonpolar residues in the presumptive pore‐lining transmembrane helix of MSL10 (TM6) that contribute to these basic channel properties. F553 and I554 are essential for wild type channel conductance and the stability of the open state. G556, a glycine residue …
Integrating Species Traits Into Species Pools, Marko J. Spasojevic, Christopher P. Catano, Joseph A. Lamanna
Integrating Species Traits Into Species Pools, Marko J. Spasojevic, Christopher P. Catano, Joseph A. Lamanna
Biology Faculty Publications & Presentations
Despite decades of research on the species‐pool concept and the recent explosion of interest in trait‐based frameworks in ecology and biogeography, surprisingly little is known about how spatial and temporal changes in species‐pool functional diversity (SPFD) influence biodiversity and the processes underlying community assembly. Current trait‐based frameworks focus primarily on community assembly from a static regional species pool, without considering how spatial or temporal variation in SPFD alters the relative importance of deterministic and stochastic assembly processes. Likewise, species‐pool concepts primarily focus on how the number of species in the species pool influences local biodiversity. However, species pools with similar …
Bioinformatics Core Competencies For Undergraduate Life Sciences Education, Sarah C.R. Elgin, Melissa Wilson - Sayers, Charles Hauser, Michael Sierk, Srebrenka Robic, Anne Rosenwald, Todd Smith, Eric W. Triplett, Jason Williams, Elizabeth Dinsdale, William Morgan, James Burnette, Samuel Donovan, Jennifer Drew, Edison Fowlks, Sebastian Galindo-Gonzales, Anya Goodman, Nealy Grandgenett, Carlos Goller, John Jungck, Jeffrey Newman, William Pearson, Elizabeth Ryder, Rafael Tosado-Acevedo, William Tapprich, Tammy Tobin, Arlin Toro-Martínez, Lonnie Welch, Robin Wright, Lindsay Barone, David Ebenbach, Mindy Mcwilliams, Kimberly Olney, Mark A. Pauley
Bioinformatics Core Competencies For Undergraduate Life Sciences Education, Sarah C.R. Elgin, Melissa Wilson - Sayers, Charles Hauser, Michael Sierk, Srebrenka Robic, Anne Rosenwald, Todd Smith, Eric W. Triplett, Jason Williams, Elizabeth Dinsdale, William Morgan, James Burnette, Samuel Donovan, Jennifer Drew, Edison Fowlks, Sebastian Galindo-Gonzales, Anya Goodman, Nealy Grandgenett, Carlos Goller, John Jungck, Jeffrey Newman, William Pearson, Elizabeth Ryder, Rafael Tosado-Acevedo, William Tapprich, Tammy Tobin, Arlin Toro-Martínez, Lonnie Welch, Robin Wright, Lindsay Barone, David Ebenbach, Mindy Mcwilliams, Kimberly Olney, Mark A. Pauley
Biology Faculty Publications & Presentations
Although bioinformatics is becoming increasingly central to research in the life sciences, bioinformatics skills and knowledge are not well integrated into undergraduate biology education. This curricular gap prevents biology students from harnessing the full potential of their education, limiting their career opportunities and slowing research innovation. To advance the integration of bioinformatics into life sciences education, a framework of core bioinformatics competencies is needed. To that end, we here report the results of a survey of biology faculty in the United States about teaching bioinformatics to undergraduate life scientists. Responses were received from 1,260 faculty representing institutions in all fifty …
Predator-By-Environment Interactions Mediate Bacterial Competition In The Dictyostelium Discoideum Microbiome, R Fredrik Inglis, Odion Asikhia, Erica Ryu, David C. Queller, Joan E. Strassmann
Predator-By-Environment Interactions Mediate Bacterial Competition In The Dictyostelium Discoideum Microbiome, R Fredrik Inglis, Odion Asikhia, Erica Ryu, David C. Queller, Joan E. Strassmann
Biology Faculty Publications & Presentations
Interactions between species and their environment play a key role in the evolution of diverse communities, and numerous studies have emphasized that interactions among microbes and among trophic levels play an important role in maintaining microbial diversity and ecosystem functioning. In this study, we investigate how two of these types of interactions, public goods cooperation through the production of iron scavenging siderophores and predation by the social amoeba Dictyostelium discoideum, mediate competition between two strains of Pseudomonas fluorescens that were co-isolated from D. discoideum. We find that although we are able to generally predict the competitive outcomes between …
An Insoluble Iron Complex Coated Cathode Enhances Direct Electron Uptake By Rhodopseudomonas Palustris Tie-1, Karthikeyan Rengasamy, Tahina Ranaivoarisoa, Rajesh Singh, Arpita Bose
An Insoluble Iron Complex Coated Cathode Enhances Direct Electron Uptake By Rhodopseudomonas Palustris Tie-1, Karthikeyan Rengasamy, Tahina Ranaivoarisoa, Rajesh Singh, Arpita Bose
Biology Faculty Publications & Presentations
Microbial electrosynthesis (MES) is a promising bioelectrochemical approach to produce biochemicals. A previous study showed that Rhodopseudomonas palustris TIE-1 can directly use poised electrodes as electron donors for photoautotrophic growth at cathodic potentials that avoid electrolytic H2 production (photoelectroautotrophy). To make TIE-1 an effective biocatalyst for MES, we need to improve its electron uptake ability and growth under photoelectroautotrophic conditions. Because TIE-1 interacts with various forms of iron while using it as a source of electrons for photoautotrophy (photoferroautotrophy), we tested the ability of iron-based redox mediators to enhance direct electron uptake. Our data show that soluble iron cannot …
Kelch F-Box Protein Positively Influences Arabidopsis Seed Germination By Targeting Phytochrome-Interacting Factor1, Manoj Majee, Santosh Kumar, Praveen Kumar Kathare, Shuiqin Wu, Derek Gingerich, Nihar R. Nayak, Louai Salaita, Randy Dinkins, Kathleen Martin, Michael Goodin, Lynnette M A Dirk, Taylor D. Lloyd, Ling Zhu, Joseph Chappell, Arthur G. Hunt, Richard D. Vierstra, Enamul Huq, A Bruce Downie
Kelch F-Box Protein Positively Influences Arabidopsis Seed Germination By Targeting Phytochrome-Interacting Factor1, Manoj Majee, Santosh Kumar, Praveen Kumar Kathare, Shuiqin Wu, Derek Gingerich, Nihar R. Nayak, Louai Salaita, Randy Dinkins, Kathleen Martin, Michael Goodin, Lynnette M A Dirk, Taylor D. Lloyd, Ling Zhu, Joseph Chappell, Arthur G. Hunt, Richard D. Vierstra, Enamul Huq, A Bruce Downie
Biology Faculty Publications & Presentations
Seeds employ sensory systems that assess various environmental cues over time to maximize the successful transition from embryo to seedling. Here we show that the Arabidopsis F-BOX protein COLD TEMPERATURE-GERMINATING (CTG)-10, identified by activation tagging, is a positive regulator of this process. When overexpressed (OE), CTG10 hastens aspects of seed germination. CTG10 is expressed predominantly in the hypocotyl, and the protein is localized to the nucleus. CTG10 interacts with PHYTOCHROME-INTERACTING FACTOR 1 (PIF1) and helps regulate its abundance in planta. CTG10-OE accelerates the loss of PIF1 in light, increasing germination efficiency, while PIF1-OE lines fail to complete germination in …
Proteasome Storage Granules Protect Proteasomes From Autophagic Degradation Upon Carbon Starvation, Richard S. Marshall, Richard D. Vierstra
Proteasome Storage Granules Protect Proteasomes From Autophagic Degradation Upon Carbon Starvation, Richard S. Marshall, Richard D. Vierstra
Biology Faculty Publications & Presentations
26S proteasome abundance is tightly regulated at multiple levels, including the elimination of excess or inactive particles by autophagy. In yeast, this proteaphagy occurs upon nitrogen starvation but not carbon starvation, which instead stimulates the rapid sequestration of proteasomes into cytoplasmic puncta termed proteasome storage granules (PSGs). Here, we show that PSGs help protect proteasomes from autophagic degradation. Both the core protease and regulatory particle sub-complexes are sequestered separately into PSGs via pathways dependent on the accessory proteins Blm10 and Spg5, respectively. Modulating PSG formation, either by perturbing cellular energy status or pH, or by genetically eliminating factors required for …
The Life Of A Microtubule, Ram Dixit, Sabine Petry
The Life Of A Microtubule, Ram Dixit, Sabine Petry
Biology Faculty Publications & Presentations
The Minisymposium “The Life of a Microtubule: Birth, Dynamics and Function” highlighted new findings on how microtubules (MTs) are made, how their length and spatial organization is regulated, and finally how they contribute to cellular functions.
Overdispersed Spatial Patterning Of Dominant Bunchgrasses In Southeastern Pine Savannas, Katherines A. Hovanes, Kyle E. Harms, Paul R. Gagnon, Jonathan A. Myers, Bret D. Elderd
Overdispersed Spatial Patterning Of Dominant Bunchgrasses In Southeastern Pine Savannas, Katherines A. Hovanes, Kyle E. Harms, Paul R. Gagnon, Jonathan A. Myers, Bret D. Elderd
Biology Faculty Publications & Presentations
Spatial patterning is a key natural history attribute of sessile organisms that frequently emerges from and dictates potential for interactions among organisms. We tested whether bunchgrasses, the dominant plant functional group in longleaf pine savanna groundcover communities, are nonrandomly patterned by characterizing the spatial dispersion of three bunchgrass species across six sites in Louisiana and Florida. We mapped bunchgrass tussocks of >5.0 cm basal diameter in three [Formula: see text] plots at each site. We modeled tussocks as two-dimensional objects to analyze their spatial relationships while preserving sizes and shapes of individual tussocks. Tussocks were overdispersed (more regularly spaced than …
New Classification Of The Dictyostelids, Sanea Sheikh, Mats Thulin, James C. Cavender, Ricardo Escalante, Shin-Ichi Kawakami, Carlos Lado, John C. Landolt, Vidyanand Nanjundiah, David C. Queller, Joan E. Strassmann, Frederick W. Spiegel, Steven L. Stephenson, Eduardo M. Vadell, Sandra L. Baldauf
New Classification Of The Dictyostelids, Sanea Sheikh, Mats Thulin, James C. Cavender, Ricardo Escalante, Shin-Ichi Kawakami, Carlos Lado, John C. Landolt, Vidyanand Nanjundiah, David C. Queller, Joan E. Strassmann, Frederick W. Spiegel, Steven L. Stephenson, Eduardo M. Vadell, Sandra L. Baldauf
Biology Faculty Publications & Presentations
Traditional morphology-based taxonomy of dictyostelids is rejected by molecular phylogeny. A new classification is presented based on monophyletic entities with consistent and strong molecular phylogenetic support and that are, as far as possible, morphologically recognizable. All newly named clades are diagnosed with small subunit ribosomal RNA (18S rRNA) sequence signatures plus morphological synapomorphies where possible. The two major molecular clades are given the rank of order, as Acytosteliales ord. nov. and Dictyosteliales. The two major clades within each of these orders are recognized and given the rank of family as, respectively, Acytosteliaceae and Cavenderiaceae fam. nov. in Acytosteliales, and Dictyosteliaceae …
Elucidating A Role For The Cytoplasmic Domain In The Mycobacterium Tuberculosis Mechanosensitive Channel Of Large Conductance, Nadia Herrera, Grigory Maksaev, Elizabeth S. Haswell, Douglas C. Rees
Elucidating A Role For The Cytoplasmic Domain In The Mycobacterium Tuberculosis Mechanosensitive Channel Of Large Conductance, Nadia Herrera, Grigory Maksaev, Elizabeth S. Haswell, Douglas C. Rees
All Faculty Publications
Microbial survival in dynamic environments requires the ability to successfully respond to abrupt changes in osmolarity. The mechanosensitive channel of large conductance (MscL) is a ubiquitous channel that facilitates the survival of bacteria and archaea under severe osmotic downshock conditions by relieving excess turgor pressure in response to increased membrane tension. A prominent structural feature of MscL, the cytoplasmic C-terminal domain, has been suggested to infuence channel assembly and function. In this report, we describe the X-ray crystal structure and electrophysiological properties of a C-terminal domain truncation of the Mycobacterium tuberculosis MscL (MtMscLΔC). A crystal structure of MtMscLΔC solubilized in …
Draft Genome Sequence Of Streptomyces Sp. Strain Jv178, A Producer Of Clifednamide-Type Polycyclic Tetramate Macrolactams, Yunci Qi, John M. D’Alessandro, Joshua A.V Blodgett
Draft Genome Sequence Of Streptomyces Sp. Strain Jv178, A Producer Of Clifednamide-Type Polycyclic Tetramate Macrolactams, Yunci Qi, John M. D’Alessandro, Joshua A.V Blodgett
Biology Faculty Publications & Presentations
Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.