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Ultrastructural Alterations Of Golgi Apparatus In The Nerve Cells Of Cerebral Cortex In Human Hydrocephalus. A Qualitative Study Using Cortical Biopsies, Orlando J. Castejon, Melecio Diaz, Consuelo Valero
Ultrastructural Alterations Of Golgi Apparatus In The Nerve Cells Of Cerebral Cortex In Human Hydrocephalus. A Qualitative Study Using Cortical Biopsies, Orlando J. Castejon, Melecio Diaz, Consuelo Valero
Scanning Microscopy
Cortical biopsies of 17 patients with clinical diagnosis of hydrocephalus and associated pathology were examined in the transmission electron microscope. Moderately and severely edematous neurons showed relevant structural changes of the Golgi apparatus consisting of either discrete or marked dilation, fragmentation, and partial disappearance of Golgi stacked cisternae. In Arnold-Chiari malformation small Golgi complexes of vesicular type and atrophic changes were observed in severely edematous neurons. The microtubules appeared intact, suggesting differential response between Golgi complex and microtubules. Atrophic changes of the Golgi complex coexisted with degenerated presynaptic endings.
Scanning Electron Microscopy Of Vertebrate Cerebellar Cortex, Orlando J. Castejón
Scanning Electron Microscopy Of Vertebrate Cerebellar Cortex, Orlando J. Castejón
Scanning Microscopy
In this study, the Golgi method for light microscopy, transmission and conventional scanning electron microscopy, the ethanol-cryofracturing technique, the freeze-fracture method for SEM, and the freeze-etching method have been used in conjunction to analyze the three-dimensional cytoarchitectonic arrangement and intracortical circuits of vertebrate cerebellar cortex. Approximately more than 100 specimens of mice, rat, teleost fishes and human cerebelli were processed by the above mentioned techniques. A chronological review of other methods for studying hidden surfaces of cerebellar nerve cell has been also described. The three-dimensional morphology, outer and inner surfaces of granule, Golgi, Purkinje and stellate cells were reviewed by …
The Growth Of Fetal Human Sensory Ganglion Neurons In Culture: A Scanning Electron Microscopic Study, Seung U. Kim, Kookmin M. Kim, Giuseppe Moretto, James H. Kim
The Growth Of Fetal Human Sensory Ganglion Neurons In Culture: A Scanning Electron Microscopic Study, Seung U. Kim, Kookmin M. Kim, Giuseppe Moretto, James H. Kim
Scanning Electron Microscopy
Sensory neurons of 8-week human fetal dorsal root ganglia were dissociated into single cells by trypsinization and cultured on coverslips for 4 months, in either serum-containing or serum-free chemically defined media. At different times the cultures were fixed and prepared for scanning electron microscopy. Fetal sensory neurons in culture regenerated axons within 24 hours which were characterized by axonal growth cones at their tips and the neuronal perikarya assumed spherical or a bonnet-like morphology.