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Full-Text Articles in Life Sciences
In Vitro Evaluation Of Anti-Eimeria Tenella, Gamont-Specific, Monoclonal Antibodies And Partial Characterization Of Their Target Antigens, Eric Wilson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study represents an effort to interrupt the life cycle of Eimeria tenella, the parasite that causes cecal coccidiosis in chickens, with a panel of 12 gamont-specific monoclonal antibodies (Mabs). To facilitate Mab screening, it was first necessary to develop a cell culture-adapted strain of E. tenella (field strain 80) from primary chicken kidney cells (PCKC) to white Leghorn chickens and back to PCKC. This alternation was repeated through 12 such passages. As a result, we have developed a cell culture-adapted strain of E. tenella that produces over 280% more oocysts in vitro than the original parent strain, and …
Functional Study Of The Structural Vp6 Protein Of Bluetongue Virus, Emiko Hayama
Functional Study Of The Structural Vp6 Protein Of Bluetongue Virus, Emiko Hayama
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study was undertaken to investigate the structure-function relationship of VP6 protein of bluetongue virus (BTV) using molecular cloning techniques. VP6 is present in small quantities in BTV and its enzymatic activity and role in the viral replication cycle have not been studied. Since the availability of large amounts of purified VP6 is essential for the analysis of VP6, a BTV-11 S3 gene was cloned into a prokaryotic protein expression system. VP6 protein was expressed in large amounts and purified to near homogeneity. A series of C-terminal and internal deletion mutants of S3 gene was constructed and the truncated VP6 …