Life Sciences Commons^™

The Characteristic Structural Features Of The Blood Vessels Of The Lewis Lung Carcinoma (A Light Microscopic And Scanning Electron Microscopic Study), T. W. Grunt, A. Lametschwandtner, K. Karrer

Scanning Electron Microscopy

Vascular corrosion casts of Lewis lung carcinomas (LLC) grown subcutaneously in C57BL/6-mice are correlated with histological sections and with tumor tissue prepared for scanning electron microscopy (SEM). By making low, medium and high pressure cast preparations we studied the influence of perfusion and injection pressure on the resulting cast sample.

Three types of vascular proliferations are distinguishable in LLC: 1) Small globular outgrowths on sinusoidal dilated tumor capillaries, caused by proliferation of their endothelial cells. 2) New sprouts on surrounding host vessels, invading the small, still avascular implant. 3) Superficially located, centrifugally running sprouts in peripheral regions of large tumors. …

The Angioarchitecture Of The Lewis Lung Carcinoma In Laboratory Mice (A Light Microscopic And Scanning Electron Microscopic Study), T. W. Grunt, A. Lametschwandtner, K. Karrer, O. Staindl

Scanning Electron Microscopy

53 Lewis lung carcinomas implanted subcutaneously into C57BL/6-mice were examined. The animals were killed at various stages of tumor growth (TG) and prepared for histology and for scanning electron microscopy (critical-point-dried tissue; vascular corrosion casts). Prior to casting animals were rinsed using different perfusion pressures. Casting was done by manual injection of the resin, whereby different influx-rates were applied resulting in low, medium and high pressure preparations.

We discern 3 phases of tumor angiogenesis (TA) occurring during 4 stages of TG among which vasodilation establishes the first reaction of the host vascular system to a growing tumor implant. During this …

Technical Parameters Of Plastics (Mercox Cl-2b And Various Methylmethacrylates) Used In Scanning Electron Microscopy Of Vascular Corrosion Casts, T. Weiger, A. Lametschwandtner, P. Stockmayer

Scanning Electron Microscopy

The most frequently used resins for vascular corrosion casting Mercox Cl-2B, Mercox Cl-2B diluted with methylmethacrylate (MMA) monomer and various self prepared MMA and hydroxyproyl-methacrylate mixtures were tested with regard to their thermostability, shrinkage, viscosity and replication quality. It was found that tempering of the plastics improves their thermostability with the exception of Mercox Cl-2B and that shrinkage depends on the amount of monomers a resin contains. In detail; Mercox Cl-2B has the lowest shrinkage (8.018%) whereas a hydroxypropyl-methacrylate mixture possessed the highest (20.408%). But, on the other hand, viscosity decreases with the quantity of monomers. All resins tested were …