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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Biology

Utah State University

Journal

1986

A-O-D-O method

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Intracellular Structure Of The Outer Hair Cell Of The Organ Of Corti, Y. Harada, T. Sakai, N. Tagashira, M. Suzuki Jun 1986

Intracellular Structure Of The Outer Hair Cell Of The Organ Of Corti, Y. Harada, T. Sakai, N. Tagashira, M. Suzuki

Scanning Electron Microscopy

The intracellular structure of the outer hair cells of the normal guinea pig organ of Corti was investigated three dimensionally by scanning electron microscope. Freeze fracturing technique followed by maceration with a 0.1% OsO4 solution (osmic maceration method) was used. Among the cell organelles, the endoplasmic reticulum (ER) showed the most interesting features, such as subsurface cisternae and lamellar bodies. The subsurface cisterna which formed a stratiform network covered the inner surface of the cell membrane and the stratiform structure disappeared at the infranuclear region. Variously shaped mitochondria (spherical, cylindrical and branched) were found on the innermost layer of …


Scanning Electron Microscopic Observation Of The Crista Ampullaris, Y. Harada, M. Takumida, N. Tagashira Jun 1986

Scanning Electron Microscopic Observation Of The Crista Ampullaris, Y. Harada, M. Takumida, N. Tagashira

Scanning Electron Microscopy

The crista ampullaris of the guinea pig and the bull frog were investigated by scanning electron microscopy. The crista ampullaris were freeze fractured or sheared followed by maceration with 0.1% OsO4 solution. Following this, three-dimensional intracellular structures were observed. The mitochondria of the sensory cells varied in shape from globular to long and slender. Golgi apparatus and endoplasmic reticulum of the sensory cells were also demonstrated clearly. Nerve elements, nerve endings and synaptic structures were also observed stereoscopically.