Life Sciences Commons^™

Adherent-Invasive Escherichia Coli (Aiec) Promotes Mild Colitis In The C57bl/6 Il-10 Ko Mouse Model Of Crohn's Disease, Sarah Sm Zaw

Senior Theses

Crohn's disease (CD) is a chronic inflammatory bowel disease. Interleukin-10 knockout (IL-10 KO) mice are crucial in CD research. Adherent-invasive Escherichia coli (AIEC) is a subgroup of E. coli. In one study, IL-10 KO mice colonized with AIEC developed colitis in the distal colon compared to wild-type (WT) mice. I investigated how AIEC NC101 WT affects colitis severity in IL-10 KO mice of the C57BL/6 genetic background. Similar results in both genotypes would enhance mouse strain accessibility. Germ-free C57BL6 IL-10 KO mice were divided into groups: colonized with AIEC NC101 for 12, 15, or 18 weeks, or kept germ-free. Histopathological …

Using Podcasts To Bring National Estuarine Research Reserves Into The Classroom For Grades 6-12, Kaitlyn M. Dirr

Senior Theses

In a typical classroom setting, there are significant challenges to exposing students to concepts related to earth sciences and the environment. These challenges are exacerbated when conveying lessons about geographic areas with limited access, such as oceans and coastlines (Louv, 2010). It is now more important than ever for environmental education to improve and adapt to our changing world. Educators may have the opportunity to bring these subjects to life by using media content such as podcasts to introduce students to new places and the scientists, managers and educators that work in those spaces. In the United States, there exists …

Local Translation And Focal Adhesions Are Dysregulated In Down Syndrome, Ashlyn Gotberg, Nikita Kirkise M.S., Kristy Welshhans Ph.D.

Senior Theses

During cellular migration, fibroblasts sense extracellular cues through membrane complexes at their leading edge and can respond to these cues via local translation. Local translation is the process in which mRNAs are transported to subcellular regions and translated in response to specific cues. There is evidence that local translation may be dysregulated in the neurodevelopmental disorder, Down syndrome, which is caused by the triplication of chromosome 21 and results in genome-wide dysregulation of protein expression. To determine if dysregulated local translation contributes to Down syndrome, we used three sets of primary fibroblasts from individuals with Down syndrome and apparently healthy …

Functional Analysis Of Stn1 Winged-Helix (Wh) Domains, Anna Bazell

Senior Theses

CST (CTC1-STN1-TEN1) is a heterotrimeric RPA-like protein complex that preferentially binds to single-stranded DNA (ssDNA). CST is conserved from yeast to humans and has been found to play a role in telomere maintenance, DNA replication, and DNA repair. Mutations in CST, specifically in the subunits of CTC1 and STN1, have been implicated in the development of the genetic diseases Coats plus and dyskeratosis congenita. Despite the known functions of CST, the mechanistic relationship between its structure and function has yet to be characterized, particularly with respect to its protein-protein interactions and DNA binding. An X-ray crystallography structure of CST revealed …

Ahr Expression On Rorc-Expressing Immune Cells Is Essential For I3c-Mediated Protection Against Colitis, Michal C. Williams

Senior Theses

Colitis is an inflammatory bowel disorder (IBD) whose etiology is attributed to modification in the luminal microbiota and dysregulation in the immune response. Indole is a signaling molecule which is naturally produced by gut luminal microbiota. Indole-3- carbinol (I3C) is a compound commonly found in vegetables and a ligand for the aryl hydrocarbon receptor (AhR). Previous studies have detected decreased expression and activation on the AhR receptor in colitis patients, thought to possibly alter gut microbiota metabolism, subsequently promoting colitis. 1 AhR, expressed in a variety of immune and epithelial cells, contributes to gut homeostasis by affecting vital mediators such …

Cyclic Fluctuations Of Zooplankton Dynamics In A Tidal Salt-Marsh Basin, Jamaal Jacobs

Senior Theses

Zooplankton are pelagic aquatic animals that are limited in their movements by the water currents around them and are major links in aquatic food webs between primary producers and higher trophic levels. Their populations are temporally and spatially variable, as they are sensitive to changes in salinity, temperature, and dissolved oxygen. This is especially true in highly dynamic environments, like estuaries, where environmental conditions are highly variable across seasonal and daily cycles. In order to examine the variability in zooplankton populations across a tidal cycle, we collected zooplankton samples from North Inlet Estuary at 30-minute intervals over a half tidal …

The Effects Of A New Mutation In Lis1 And Its Potential Role In Autism Spectrum Disorder, John A. Slovensky

Senior Theses

LIS1 is a protein, particularly concentrated in the brain, that is important in regulating the movement and transport capabilities of dynein. Dynein is protein that can move along pathways in the cell, carrying various cellular components and also helping brain cells migrate. These two proteins are crucial during the development of the nervous system, and mutations in them can lead to lissencephaly, a disorder in which the brain does not form properly. This disease causes patients to develop cognitive-motor defects and seizures that can lead to early death. While all previously identified mutations in LIS1 are known to cause lissencephaly, …

Cellular Localization Of Rad51d Mutant Proteins And The Application Of Art To Increase Scientific Literacy In America, Claire L. Chabot

Senior Theses

Ovarian cancers are the leading cause of death from cancer of the female reproductive system. Approximately 50% of ovarian cancers have defects in the homologous recombination (HR) DNA repair pathway that is required for the repair of DNA double-stranded breaks. The status of HR genes, such as BRCA1, BRCA2, and the RAD51 family, contributes to ovarian cancer development as well as treatment decisions regarding chemotherapy, radiation, and immunotherapy. The overarching goal of this project is to identify new insights into HR that can integrate with Precision Medicine Initiatives and align with the goals of the Cancer Moonshot 2020 Program. I …

Sonification Of Epigenetic Processes, Joelle Strom

Senior Theses

Sonification is the process of creating sound or music from data for investigative or aesthetic purposes. Data sonification has been used in multiple fields to provide an additional layer of analysis to datasets, engage general audiences in a scientific topic, or create an evocative musical piece. Sonification has been extended to biological subjects, including macromolecules such as protein and DNA. Epigenetics, the processes by which gene expression is regulated, is a burgeoning field of molecular biology research. Epigenetic processes have been scarcely sonified, and the process of sonifying DNA base methylation has never been published. DNA methylation is an integral …

Hallmark Features Of Stem Anatomy In The Family Lamiaceae, Catherine Garner

Senior Theses

Lamiaceae is a cosmopolitan family, containing around 236 genera and 7,534 species. The family has been the object of considerable taxonomic controversy and ultimate revision, especially concerning subfamilial and tribal limits. A broad-based anatomical approach has been undertaken to assess comparative anatomy of the stem as a reasonable systematic technique. The stems of several members of the family were compared. Cross-sections were made using the recently invented hydromicrotome, fixed, and examined at magnifications of 40x, 100x, and 400x. The common anatomical features of each sectioned species were determined, and used to establish a basic guide to the hallmark stem features …

Assessing Variation In Visual Abilities In Daphnia As A Result Of Disparity In Eye Sizes, Adrian Perez

Senior Theses

The functionality of an eye is affected by a number of structural factors, and altering any of these factors often involves inevitable tradeoffs between resolution and sensitivity. However, it has been theoretically reasoned, and tested empirically, that increasing the size of an eye allows for both of these central features of vision to be improved due to corresponding increases in lens size. This study examines the relevance of this hypothesis for the vision of Daphnia in both interspecific and intraspecific contexts. The visual capabilities of six different species of Daphnia are tested using the optomotor response—a visually mediated behavior that …

An Illustrated Investigation Of Agraulis Vanillae And Its Larval Food Plants, Christiana M. Keinath

Senior Theses

To create my work, I directly observed caterpillars, chrysalises, and adult butterflies. I used a variety of media to depict salient aspects of gulf fritillary anatomy and to follow the species through its life stages in connection with its passionflower hosts. I focused on accurately recreating what I observed, as well as breaking down the organisms into their component colors, lines, shapes, and values. In my work I emphasized natural textures, which vary greatly as the caterpillars undergo their morphological changes. As a result of this project, I improved my observation and life drawing skills and learned how the process …

Scaling Of Anatomically Derived Maximal Bite Force In Primates, Ashley Deutsch

Senior Theses

Bite force analysis is important the examination of the evolutionary factors that have shaped the masticatory apparatus. Unfortunately, in primates, empirically measured bite force is largely unavailable because of the invasive nature of the procedure. Because of this, we have estimated bite force in a large sample across the primate order at three different realistic bite points along the tooth row: the first maxillary incisor, the maxillary canine, and the paracone of the first maxillary molar. Here, we model bite force by combining measured physiological crosssectional area of the jaw adductors from Hartstone-Rose and colleagues (2017) with osteological measurements from …

Epigenetic Regulation Of Salicylic Acid-Mediated Plant Defense, Phillip Key

Senior Theses

Plant pathogens remain a significant threat to the stability of modern agricultural systems, and the investigation of mechanisms to improve the security of food resources has led to the partial characterization of plant immune response. With this progress, there is a newfound ability to analyze the relative pathogen resistance capability of specifically modified organisms, and in doing so, it is possible to identify individual alterations that might play a role in creating a more robust immune response. In this study, ten Arabidopsis thaliana mutants were infiltrated with Pseudomonas syringae pv. morsprunorum alongside positive and negative control Col-0 Wild-Type and npr1-2. …

Tree Damage From Hurricane Matthew Affecting Forest Dynamics In Congaree National Forest, Connor Lentz

Senior Theses

The Congaree National Forest serves as an ecological model for bottomland-hardwood forests. Hurricanes periodically strike the Southeast United States, with high-wind speeds causing damage affecting the Congaree National Forest and modifying forest dynamics. This has been well-studied from the effects of Hurricane Hugo in September 1989 (Sharitz et al. 1993). In October 2016, Hurricane Matthew, a category 5 storm, made landfall in the Southeastern United States, and eventually moved through the Congaree National Forest. The high winds and precipitation caused widespread tree damage throughout Congaree. This study used a belt-transect survey of 14.7km of trails throughout the park to record …

The Effects Of Low-Dose Ionizing Radiation On Voles From The Chernobyl Exclusion Zone, Justin Sindoni

Senior Theses

Much is known about the effects of high doses of ionizing radiation exposure, but little is known about the effects of low doses of ionizing radiation over extended periods of time. Nuclear power plant disasters, in particular the 1986 Chernobyl disaster, have led to long-term radioactive contamination of their surrounding areas, and more research is needed to learn about the effects of the contamination. In order to better understand the impacts of low-dose ionizing radiation on animal populations, bank voles (Myodes glareolus) from the Chernobyl exclusion zone were studied. Voles were captured from 64 sites throughout the exclusion zone, and …

The Effects Of Β2 Adrenergic Receptor Activation On Macrophage Cholesterol Accumulation And Inflammatory Responses, Crystal Alece Wood

Senior Theses

With the number of cases of cardiovascular diseases, which are most commonly caused by atherosclerosis, continuously rising, it is important to see how different lifestyle factors can contribute to one’s health. If limiting the amount of social stress an individual experiences can cause a decrease in the prevalence of these diseases, it will be important for physicians to encourage patients to avoid possible stressors or determine ways to cope with psychosocial stress in order to decrease their risks for such diseases. In this study, Clenbuterol, a β2 adrenergic agonist, was used to activate the β2 adrenergic receptor on macrophages in …

Design Proposal: Peer Mentoring Program In Schc, Kylie Anna Stevens

Senior Theses

Peer mentoring programs have been shown to be successful at universities across the country. This present paper proposes the creation of a peer mentoring program within the South Carolina Honors College. This program has been designed to enhance the first-year experience for incoming Honors College freshmen by pairing them with an upperclassmen student mentor that can help guide them through their first year and beyond. The role of these mentors would be to provide valuable guidance for students on how to be successful as a student of the Honors College and the University of South Carolina. This mentoring program aims …

Tales Of The Atom!: An Analysis Of Nuclear Discourage In Early Marvel Comics, Kelly Elizabeth Shepherd

Senior Theses

In 1961 the editor of Atlas Comics, Martin Goodman, approached his nephew and staff writer Stan Lee with a pitch. Goodman wanted Lee to create a super hero team to rival DC Comics’, Justice League of America. Lee came up with the Fantastic Four who debuted in November of 1961. The super hero team led by physicist Bruce Banner was the first of many successful titles from Marvel Comics. Much of the popularity of Marvel Comics stemmed from the company’s focus on the characters rather than only action like other superhero comics at the time. Lee developed characters that …

Challenging Authoritative Perceptions: The Story Of Philip James Evans, Michael Nicklas Goyda

Senior Theses

This senior thesis is an extension on a previous work I have done for Honors Creative Writing (ENGL 360). The story takes you through the early life of Philip James Evans, a young man born in 1976 in Pittsburgh, PA. An alcoholic in remission, he is killed one day and wakes up in a mysterious building which is actually the headquarters of The Illuminati in Purgatory. Philip faces a tough decision: remain dead and await judgment for his wrongdoings and risk being sent to Hell, or work for The Illuminati to pay off the debt of his sins and receive …

The Vascular Flora Of The Gills Creek Watershed, Austin Corbett Williams

Senior Theses

This is a floral inventory of the Gills creek watershed. It was conducted from fall 2014 through spring 2016. The final product is a list of plants that are known to presently or historically exist in the watershed. The list is comprised of 250 native species and 36 introduced and invasive species. The results indicate that Gills creek watershed’s plant diversity is severely threatened by introduced and invasive species, but still retains sizable native diversity.

Foodshare Columbia: Fostering A Healthier Community, Mavinder Kaur Guram

Senior Theses

For my thesis project, I developed a wholesale produce co-op to provide fresh produce to low-income families in Columbia with Dr. Gregg Talente and Ms. Beverly Wilson at the USC School of Medicine. Low-income populations lack access to healthy foods due not only to cost, but also limited proficiencies in skills required to prepare healthy foods. In an effort to address these issues, FoodShare Columbia was created. A community-based co-op, FoodShare was formed to foster a healthier community by improving access to nutritious, affordable foods. The program functions through the use of a Fresh Food Box (box of fresh produce …

The Effect Of The Elevated Circulating Levels Of Il-33 On Metastasis Of Colorectal Cancer, Christina-Lin Marie Brown

Senior Theses

In the United States, colorectal cancer is the third leading cause of cancer-related deaths in both men and women¹. The pathogenesis of colorectal cancer begins with the development of polyps in the innermost colorectal lining and progresses to the final stage when metastasis occurs². Metastasis is the spread of cancer cells to other organs, and has been shown to be organ specific rather than a random process. Genes that direct tumor cells to target specific organs have been identified. The primary tumor is thought to secrete molecules that promote the establishment of liver metastasis even before …

Deeply Rooted: The Story Of Congaree National Park, Taylor Karlin

Senior Theses

This photo-essay book was written to fulfill requirements for completion of a senior thesis project to graduate with honors from the South Carolina Honors College. Its purpose is to promote the enjoyment and preservation of the Congaree National Park by educating the public on the historical and ecological significance of the land and the many activities it has to offer. Despite its proximity to downtown Columbia, there remains a lack of public awareness about the park and the amazing activities and opportunities it has to offer. I have compiled information and pictures that will best depict the unique qualities of …

Genome Sequence And Phenotypic Characterization Of Caulobacter Segnis, Sagar Patel

Senior Theses

Caulobacter segnis is a unique species of Caulobacter in that it is slow growing, found in soil samples, and has other phenotypic differences from the freshwater Caulobacter that it is most closely related to. Initially deemed Mycoplana segnis, it was reclassified after a 16s rRNA analysis and found to be most closely related to the Caulobacter NA1000 branch. Because the annotated sequence available in GenBank contained a large number of pseudogenes (126), we compared the original sequencing data to the GenBank sequence and determined that many of the pseudogenes were due to sequence errors in the Genbank sequence. Consequently, …

Determining A Correlation Between Individual Differences In Eye Movements And Working Memory, Mackenzie Sunday

Senior Theses

This study determined if a relationship exists between individual differences in eye movements and working memory measures. The eye movement system can provide insight into processes that occur in the mind as well as a better understanding of the relationship between quantifiable aspects of eye movements and the more abstract inner workings of the mind. Recently, consistent and reliable individual differences have been found in individuals’ eye movement behaviors. For example, individuals with longer fixation durations for one visual task have longer fixations across all other visual tasks. The eye movements of participants were collected during four different viewing tasks …

The Role Of Lipocalin 2 In Early Stage Colon Cancer Metastasis To The Liver, Fides Elamparo

Senior Theses

Metastasis, frequently from the colon to the liver, is the major cause of death with colorectal cancer, reducing the five-year survival to less than 6%. Metastasis occurs due to productive collaborations between tumor cells and host-derived cells in the tumor microenvironment, where a pre-metastatic niche is created to prime for cancer cell invasion into the target organ. In a highly metastatic colorectal cancer cell line implanted into the cecum of Balb/c mice, microarray analysis showed lipocalin 2 (Lcn2) is one of the most highly expressed proteins in the liver of tumor-bearing mice prior to metastasis.

When RT-PCR was performed, greater …