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Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
Editorial: Murine Models Of Leukemia And Lymphoma, Christine E. Cutucache, Pierluigi Porcu
Editorial: Murine Models Of Leukemia And Lymphoma, Christine E. Cutucache, Pierluigi Porcu
Biology Faculty Publications
Murine models serve as an effective way to mimic the in vivo tumor microenvironmental interactions that take place in patients with leukemias and lymphomas. Specifically, leukemias and lymphomas rely heavily on the surrounding stroma and tissue microenvironmental cytokine and chemokine signals to ensure survival and expansion of tumor cells. Finally, leukemic cells migrate thanks to signals from varying regions of the host, furthering the progression and severity of disease. It is therefore impossible to fully understand such a dynamic relationship between tumor cells and their surrounding microenvironment, and the events to transformation in leukemias and lymphomas without an in vivo, …
Filamentation Involves Two Overlapping, But Distinct, Programs Of Filamentation In The Pathogenic Fungus Candida Albicans, Jahaun Azadmanesh, Austin M. Gowen, Paul E. Creger, Nicole D. Shafer, Jill R. Blankenship
Filamentation Involves Two Overlapping, But Distinct, Programs Of Filamentation In The Pathogenic Fungus Candida Albicans, Jahaun Azadmanesh, Austin M. Gowen, Paul E. Creger, Nicole D. Shafer, Jill R. Blankenship
Biology Faculty Publications
The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in in vitro models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various in vitro models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 in vitro models …
Using Murine Models To Understand Tumor-Lymphoid Interactions: Spotlight On Cll And Aitl, Tyler Herek, Christine E. Cutucache
Using Murine Models To Understand Tumor-Lymphoid Interactions: Spotlight On Cll And Aitl, Tyler Herek, Christine E. Cutucache
Biology Faculty Publications
The role of the tumor microenvironment in leukemias and lymphomas is well-established, yet the intricacies of how the malignant cells regulate and influence their non-malignant counterparts remain elusive. For example, chronic lymphocytic leukemia is an expansion of malignant CD5+CD19+ B cells, yet the non-malignant T cells play just as large of a role in disease presentation and etiology. Herein, we review the dynamic tumor cell to lymphoid repertoire interactions found in two Non-Hodgkin’s lymphoma subtypes: chronic lymphocytic leukemia and angioimmunoblastic T-cell lymphoma. We aim to highlight the pivot work done in the murine models which recapitulate these diseases and explore …
Complete Genome Sequences Of Pseudomonas Fluorescens Bacteriophages Isolated From Freshwater Samples In Omaha, Nebraska, Guoqing Lu, Jamie L. Luhr, Andrew Stoecklein, Paige Warner, William Tapprich
Complete Genome Sequences Of Pseudomonas Fluorescens Bacteriophages Isolated From Freshwater Samples In Omaha, Nebraska, Guoqing Lu, Jamie L. Luhr, Andrew Stoecklein, Paige Warner, William Tapprich
Biology Faculty Publications
The complete genome sequences of four Pseudomonas fluorescens bacteriophages, UNO-SLW1 to UNO-SLW4, isolated from freshwater samples, are 39,092 to 39,215 bp long. The genomes are highly similar (identity, >0.995) but dissimilar from that of Pseudomonas phage Pf-10 (the closest relative, 0.685 to 0.686 identity), with 48 to 49 protein-coding genes and 66 regulatory sites predicted.
How Do Undergraduate Stem Mentors Reflect Upon Their Mentoring Experiences In An Outreach Program Engaging K-8 Youth?, Kari L. Nelson, Jamie Sabel, Cory Forbes, Neal Grandgenett, William E. Tapprich, Christine E. Cutucache
How Do Undergraduate Stem Mentors Reflect Upon Their Mentoring Experiences In An Outreach Program Engaging K-8 Youth?, Kari L. Nelson, Jamie Sabel, Cory Forbes, Neal Grandgenett, William E. Tapprich, Christine E. Cutucache
Biology Faculty Publications
Background: Many university students are becoming involved in mentoring programs, yet few studies describe the impact of mentoring on the mentor. Additionally, many studies report that students graduating from college are not prepared to enter the workforce in terms of key career skills and/or content knowledge. Herein, we examine the impact of our program, NE STEM 4U (Nebraska Science, Technology, Engineering and Math for You), in which undergraduate (UG) mentors engage K-8 youth in after-school STEM experiments. The UGs reflected upon their experiences using post-mentoring evaluations, 12- and 24-week interviews, and exit surveys. Many of the questions asked of …
How Do Former Undergraduate Mentors Evaluate Their Mentoring Experience 3-Years Post-Mentoring: A Phenomenological Study, Kari L. Nelson, Christine E. Cutucache
How Do Former Undergraduate Mentors Evaluate Their Mentoring Experience 3-Years Post-Mentoring: A Phenomenological Study, Kari L. Nelson, Christine E. Cutucache
Biology Faculty Publications
This phenomenological study involves a unique, longitudinal assessment of the lived experiences of former undergraduate mentors (n=7) in light of their current experiences (i.e., career or advanced schooling). The objective of a phenomenological study is to engage in in-depth probing of a representative number of participants. Specifically, we followed up with graduates of the Nebraska STEM 4U (NE STEM 4U) intervention 3 years post-program, with the overall goal of describing the mentors’ experiences using the lens of their current experiences. This type of longitudinal perspective of mentoring is greatly lacking in the current literature. At the time of the interviews, …
Detection Of Intestinal Pathogens In River, Shore, And Drinking Water In Lima, Peru, David Grothen, Sydney J. Zach, Paul H. Davis
Detection Of Intestinal Pathogens In River, Shore, And Drinking Water In Lima, Peru, David Grothen, Sydney J. Zach, Paul H. Davis
Biology Faculty Publications
Water quality management is an ongoing struggle for many locations worldwide. Current testing of water supplies can be time-consuming, expensive, and lack sensitivity. This study describes an alternative, easy-to-use, and inexpensive method to water sampling and testing at remote locations. This method was employed to detect a number of intestinal pathogens in various locations of Lima, Peru. A total of 34 PCR primer pairs were tested for specificity and high-yield amplification for 12 different pathogens using known DNA templates. Select primers for each pathogen were then tested for minimum detection limits of DNA. Water samples were collected from 22 locations. …
Predicting Hiv Pre-Exposure Prophylaxis Efficacy For Women Using A Preclinical Pharmacokinetic-Pharmacodynamic In Vivo Model, Angela Wahl, Phong T. Ho, Paul W. Denton, Katy L. Garrett, Michael G. Hudgens, Glenn Swartz, Cynthia O'Neill, Fulvia Veronese, Angela D. Kashuba, J. Victor Garcia
Predicting Hiv Pre-Exposure Prophylaxis Efficacy For Women Using A Preclinical Pharmacokinetic-Pharmacodynamic In Vivo Model, Angela Wahl, Phong T. Ho, Paul W. Denton, Katy L. Garrett, Michael G. Hudgens, Glenn Swartz, Cynthia O'Neill, Fulvia Veronese, Angela D. Kashuba, J. Victor Garcia
Biology Faculty Publications
The efficacy of HIV pre-exposure prophylaxis (PrEP) relies on adherence and may also depend on the route of HIV acquisition. Clinical studies of systemic tenofovir disoproxil fumarate (TDF) PrEP revealed reduced efficacy in women compared to men with similar degrees of adherence. To select the most effective PrEP strategies, preclinical studies are critically needed to establish correlations between drug concentrations (pharmacokinetics [PK]) and protective efficacy (pharmacodynamics [PD]). We utilized an in vivo preclinical model to perform a PK-PD analysis of systemic TDF PrEP for vaginal HIV acquisition. TDF PrEP prevented vaginal HIV acquisition in a dose-dependent manner. PK-PD modeling of …
Fostering Curiosity, Inquiry, And Scientific Thinking In Elementary School Students: Impact Of The Ne Stem 4u Intervention, Heather Leas, Kari L. Nelson, Neal Grandgenett, William E. Tapprich, Christine E. Cutucache
Fostering Curiosity, Inquiry, And Scientific Thinking In Elementary School Students: Impact Of The Ne Stem 4u Intervention, Heather Leas, Kari L. Nelson, Neal Grandgenett, William E. Tapprich, Christine E. Cutucache
Biology Faculty Publications
In this qualitative study, we assessed the impact of the NE STEM 4U intervention on elementary school youth in terms of excitement, curiosity, and STEM concepts. The NE STEM 4U intervention incorporates a problem-based learning theoretical framework in an after-school time, weekly or twice-weekly intervention. We assessed student performance over two academic years of participation in the intervention using the Dimensions of Success observational tool. Ultimately, we link this mentor-led program with increases in curiosity, inquiry, and STEM concept gains. Taken together, these findings support after-school interventions in STEM areas as key encouraging excitement in youth and motivation to pursue …