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Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel
Characterization Of Novel Chlorovirus Glycosyltransferases That Synthesize Atypical Glycans, Eric Noel
School of Biological Sciences: Dissertations, Theses, and Student Research
Giant dsDNA chloroviruses encode a diverse repertoire of glycosyltransferases (GTases) and methyltransferases (MTases) that biosynthesize unusual, methylated sugars independent of their host chlorella-like green algae prompting a reexamination of glycobiology systems. Unlike most other viruses, the prototype chlorovirus PBCV-1 encodes most, if not all, of the machinery required to glycosylate its major capsid protein (MCP). The structures of the four N-linked glycans do not resemble any other glycans in the three domains of life. Here, we investigated the potential involvement of chlorovirus- encoded putative GTases and MTases in glycosylation of the viral MCP. First, we aimed to generate site-directed …