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University of Nebraska - Lincoln
Department of Animal Science: Dissertations, Theses, and Student Research
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Transcriptional Regulation Of The Porcine Gnrh Receptor Gene By Glucocorticoids, Chanho Lee
Transcriptional Regulation Of The Porcine Gnrh Receptor Gene By Glucocorticoids, Chanho Lee
Department of Animal Science: Dissertations, Theses, and Student Research
Binding of GnRH to its receptor (GnRHR) stimulates the synthesis and secretion of the gonadotropins, as well as up-regulation of GnRHR. Thus, the interaction between GnRH and GnRHR represents a central point for regulation of reproduction. Glucocorticoids alter reproduction by reducing GnRH responsiveness of gonadotropes within the anterior pituitary gland, potentially via transcriptional regulation of the GnRHR gene. Investigation into this mechanism, however, revealed that the murine GnRHR gene was stimulated by glucocorticoids. To determine the effect of glucocorticoids on porcine GnRHR gene expression, gonadotrope-derived αT3-1 cells were transiently transfected with a vector containing 5118 bp of 5’ flanking sequence …