Open Access. Powered by Scholars. Published by Universities.®
- Publication
- Publication Type
Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
An Adult Zebrafish Brain Atlas To Investigate Shh Mediated Cell-Cell Signaling In Neurogenic Zones, Alyssa P. Lutservitz
An Adult Zebrafish Brain Atlas To Investigate Shh Mediated Cell-Cell Signaling In Neurogenic Zones, Alyssa P. Lutservitz
Masters Theses
Adult neurogenesis occurs in proliferative zones of the brain that contain neural progenitor cell populations capable of differentiating into specific cell types. However, we remain limited in our understanding of the signals that regulate neural progenitor cell proliferation and differentiation in adults. Recently zebrafish (Danio rerio) have emerged as an excellent model for studying the molecular mechanisms behind adult neurogenesis, because sixteen proliferative zones remain active in the adult brains. Thousands of fluorescent transgenic reporter lines have been generated in zebrafish that reveal gene expression patterns of cell-cell signaling systems, some of which may regulate neurogenesis in these …
Macondo Crude Oil From The Deepwater Horizon Oil Spill Disrupts Specific Developmental Processes During Zebrafish Embryogenesis, T Yvanka De Soysa, Allison Ulrich, Timo Friedrich, Danielle Pite, Shannon L. Compton, Deborah Ok, Rebecca L. Bernardos, Gerald B. Downes, Shizuka Hsieh, Rachael Stein, M Caterina Lagdameo, Katherine Halvorsen, Lydia-Rose Kesich, Michael Jf Barresi
Macondo Crude Oil From The Deepwater Horizon Oil Spill Disrupts Specific Developmental Processes During Zebrafish Embryogenesis, T Yvanka De Soysa, Allison Ulrich, Timo Friedrich, Danielle Pite, Shannon L. Compton, Deborah Ok, Rebecca L. Bernardos, Gerald B. Downes, Shizuka Hsieh, Rachael Stein, M Caterina Lagdameo, Katherine Halvorsen, Lydia-Rose Kesich, Michael Jf Barresi
Gerald B. Downes
Background: The Deepwater Horizon disaster was the largest marine oil spill in history, and total vertical exposure of oil to the water column suggests it could impact an enormous diversity of ecosystems. The most vulnerable organisms are those encountering these pollutants during their early life stages. Water-soluble components of crude oil and specific polycyclic aromatic hydrocarbons have been shown to cause defects in cardiovascular and craniofacial development in a variety of teleost species, but the developmental origins of these defects have yet to be determined. We have adopted zebrafish, Danio rerio, as a model to test whether water accumulated fractions …
Essential Genes For Astroglial Development And Axon Pathfinding During Zebrafish Embryogenesis, Michael J.F. Barresi, Sean Burton, Kristina Dipietrantonio, Adam Amsterdam, Nancy Hopkins, Rolf O. Karlstrom
Essential Genes For Astroglial Development And Axon Pathfinding During Zebrafish Embryogenesis, Michael J.F. Barresi, Sean Burton, Kristina Dipietrantonio, Adam Amsterdam, Nancy Hopkins, Rolf O. Karlstrom
Rolf O Karlstrom
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a “shelf-screen” to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were …
The Transcriptional Repressor Rest/Nrsf Modulates Hedgehog Signaling, Keith P. Gates, Laura Mentzer, Rolf O. Karlstrom, Howard I. Sirotkin
The Transcriptional Repressor Rest/Nrsf Modulates Hedgehog Signaling, Keith P. Gates, Laura Mentzer, Rolf O. Karlstrom, Howard I. Sirotkin
Biology Department Faculty Publication Series
The spatial and temporal control of gene expression is key to generation of specific cellular fates during development. Studies of the transcriptional repressor REST/NRSF (RE1 Silencing Transcription Factor or Neural Restrictive Silencing Factor) have provided important insight into the role that epigenetic modifications play in differential gene expression. However, the precise function of REST during embryonic development is not well understood. We have discovered a novel interaction between zebrafish Rest and the Hedgehog (Hh) signaling pathway. We observed that Rest knockdown enhances or represses Hh signaling in a context-dependant manner. In wild-type embryos and embryos with elevated Hh signaling, Rest …
Mosaic Eyes Is A Novel Component Of The Crumbs Complex And Negatively Regulates Photoreceptor Apical Size, Ya-Chu Hsu, John J. Willoughby, Arne K. Christensen, Abbie M. Jensen
Mosaic Eyes Is A Novel Component Of The Crumbs Complex And Negatively Regulates Photoreceptor Apical Size, Ya-Chu Hsu, John J. Willoughby, Arne K. Christensen, Abbie M. Jensen
Biology Department Faculty Publication Series
Establishment of apical-basal cell polarity has emerged as an important process during development, and the Crumbs complex is a major component of this process in Drosophila. By comparison, little is known about the role of Crumbs (Crb) proteins in vertebrate development. We show that the FERM protein Mosaic Eyes (Moe) is a novel regulatory component of the Crumbs complex. Moe coimmunoprecipitates with Ome/Crb2a and Nok (Pals1) from adult eye and in vitro interaction experiments suggest these interactions are direct. Morpholino knockdown of ome/crb2a phenocopies the moe mutations. Moe and Crumbs proteins colocalize apically and this apical localization …
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Rolf O. Karlstrom, Gary Vanderlaan, Oksana V. Tyurina, Anand Chandrasekhar
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Rolf O. Karlstrom, Gary Vanderlaan, Oksana V. Tyurina, Anand Chandrasekhar
Rolf O Karlstrom
The Gli family of zinc-finger transcription factors mediates Hedgehog (Hh) signaling in all vertebrates. However, their roles in ventral neural tube patterning, in particular motor neuron induction, appear to have diverged across species. For instance, cranial motor neurons are essentially lost in zebrafish detour (gli1−) mutants, whereas motor neuron development is unaffected in mouse single gli and some double gli knockouts. Interestingly, the expression of some Hh-regulated genes (ptc1, net1a, gli1) is mostly unaffected in the detour mutant hindbrain, suggesting that other Gli transcriptional activators may be involved. To better define the roles of the zebrafish gli genes in motor …
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Biology Department Faculty Publication Series
Three major axon pathways cross the midline of the vertebrate forebrain early in embryonic development: the postoptic commissure (POC), the anterior commissure (AC) and the optic nerve. We show that a small population of Gfap+astroglia spans the midline of the zebrafish forebrain in the position of, and prior to, commissural and retinal axon crossing. These glial `bridges' form in regions devoid of the guidance molecules slit2 and slit3,although a subset of these glial cells express slit1a. We show that Hh signaling is required for commissure formation, glial bridge formation, and the restricted expression of the guidance molecules slit1a …
Organization Of The Pronephric Filtration Apparatus In Zebrafish Requires Nephrin, Podocin And The Ferm Domain Protein Mosaic Eyes, Albrecht G. Kramer-Zucker, Stephanie Wiessner, Abbie M. Jensen, Iain A. Drummond
Organization Of The Pronephric Filtration Apparatus In Zebrafish Requires Nephrin, Podocin And The Ferm Domain Protein Mosaic Eyes, Albrecht G. Kramer-Zucker, Stephanie Wiessner, Abbie M. Jensen, Iain A. Drummond
Biology Department Faculty Publication Series
Podocytes are specialized cells of the kidney that form the blood filtration barrier in the kidney glomerulus. The barrier function of podocytes depends upon the development of specialized cell–cell adhesion complexes called slit-diaphragms that form between podocyte foot processes surrounding glomerular blood vessels. Failure of the slit-diaphragm to form results in leakage of high molecular weight proteins into the blood filtrate and urine, a condition called proteinuria. In this work, we test whether the zebrafish pronephros can be used as an assay system for the development of glomerular function with the goal of identifying novel components of the slit-diaphragm. We …
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Gary Vanderlaan, Oksana V. Tyurina, Rolf O. Karlstrom, Anand Chandrasekhar
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Gary Vanderlaan, Oksana V. Tyurina, Rolf O. Karlstrom, Anand Chandrasekhar
Biology Department Faculty Publication Series
No abstract provided.