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Belladonna/Lhx2 Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, James Culverwell, Mitchell Walkowicz, Sabrina Toro, Jens M. Rick, Stephan C.F. Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Belladonna/Lhx2 Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, James Culverwell, Mitchell Walkowicz, Sabrina Toro, Jens M. Rick, Stephan C.F. Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Rolf O Karlstrom
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross t he midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna ( bel ) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of …
Belladonna/(Lhx2) Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, Jay Culverwell, Mitch Walkowicz, Sabrina Toro, Jens M. Rick, Stephan Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Belladonna/(Lhx2) Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, Jay Culverwell, Mitch Walkowicz, Sabrina Toro, Jens M. Rick, Stephan Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Biology Department Faculty Publication Series
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross the midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna (bel) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye, and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye and that loss of bel …
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Rolf O. Karlstrom, Gary Vanderlaan, Oksana V. Tyurina, Anand Chandrasekhar
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Rolf O. Karlstrom, Gary Vanderlaan, Oksana V. Tyurina, Anand Chandrasekhar
Rolf O Karlstrom
The Gli family of zinc-finger transcription factors mediates Hedgehog (Hh) signaling in all vertebrates. However, their roles in ventral neural tube patterning, in particular motor neuron induction, appear to have diverged across species. For instance, cranial motor neurons are essentially lost in zebrafish detour (gli1−) mutants, whereas motor neuron development is unaffected in mouse single gli and some double gli knockouts. Interestingly, the expression of some Hh-regulated genes (ptc1, net1a, gli1) is mostly unaffected in the detour mutant hindbrain, suggesting that other Gli transcriptional activators may be involved. To better define the roles of the zebrafish gli genes in motor …
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Biology Department Faculty Publication Series
Three major axon pathways cross the midline of the vertebrate forebrain early in embryonic development: the postoptic commissure (POC), the anterior commissure (AC) and the optic nerve. We show that a small population of Gfap+astroglia spans the midline of the zebrafish forebrain in the position of, and prior to, commissural and retinal axon crossing. These glial `bridges' form in regions devoid of the guidance molecules slit2 and slit3,although a subset of these glial cells express slit1a. We show that Hh signaling is required for commissure formation, glial bridge formation, and the restricted expression of the guidance molecules slit1a …
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Gary Vanderlaan, Oksana V. Tyurina, Rolf O. Karlstrom, Anand Chandrasekhar
Gli Function Is Essential For Motor Neuron Induction In Zebrafish, Gary Vanderlaan, Oksana V. Tyurina, Rolf O. Karlstrom, Anand Chandrasekhar
Biology Department Faculty Publication Series
No abstract provided.
Genetic Analysis Of Zebrafish Gli1 And Gli2 Reveals Divergent Requirements For Gli Genes In Vertebrate Development, Rolf O. Karlstrom, Oksana V. Tyurina, Atsushi Kawakami, Noriyuki Nishioka, William S. Talbot, Hiroshi Sasaki, Alexander F. Schier
Genetic Analysis Of Zebrafish Gli1 And Gli2 Reveals Divergent Requirements For Gli Genes In Vertebrate Development, Rolf O. Karlstrom, Oksana V. Tyurina, Atsushi Kawakami, Noriyuki Nishioka, William S. Talbot, Hiroshi Sasaki, Alexander F. Schier
Biology Department Faculty Publication Series
Gli proteins regulate the transcription of Hedgehog (Hh) target genes. Genetic studies in mouse have shown that Gli1 is not essential for embryogenesis, whereas Gli2 acts as an activator of Hh target genes. In contrast, misexpression studies in Xenopus and cultured cells have suggested that Gli1 can act as an activator of Hh-regulated genes, whereas Gli2 might function as a repressor of a subset of Hh targets. To clarify the roles of gli genes during vertebrate development, we have analyzed the requirements for gli1 and gli2 during zebrafish embryogenesis. We report that detour (dtr) mutations encode loss-of-function alleles …