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Full-Text Articles in Life Sciences
Essential Genes For Astroglial Development And Axon Pathfinding During Zebrafish Embryogenesis, Michael J.F. Barresi, Sean Burton, Kristina Dipietrantonio, Adam Amsterdam, Nancy Hopkins, Rolf O. Karlstrom
Essential Genes For Astroglial Development And Axon Pathfinding During Zebrafish Embryogenesis, Michael J.F. Barresi, Sean Burton, Kristina Dipietrantonio, Adam Amsterdam, Nancy Hopkins, Rolf O. Karlstrom
Rolf O Karlstrom
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a “shelf-screen” to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were …
Belladonna/Lhx2 Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, James Culverwell, Mitchell Walkowicz, Sabrina Toro, Jens M. Rick, Stephan C.F. Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Belladonna/Lhx2 Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, James Culverwell, Mitchell Walkowicz, Sabrina Toro, Jens M. Rick, Stephan C.F. Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Rolf O Karlstrom
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross t he midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna ( bel ) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of …
Belladonna/(Lhx2) Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, Jay Culverwell, Mitch Walkowicz, Sabrina Toro, Jens M. Rick, Stephan Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Belladonna/(Lhx2) Is Required For Neural Patterning And Midline Axon Guidance In The Zebrafish Forebrain, Anandita Seth, Jay Culverwell, Mitch Walkowicz, Sabrina Toro, Jens M. Rick, Stephan Neuhauss, Zoltan M. Varga, Rolf O. Karlstrom
Biology Department Faculty Publication Series
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross the midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna (bel) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye, and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye and that loss of bel …
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Hedgehog Regulated Slit Expression Determines Commissure And Glial Cell Position In The Zebrafish Forebrain, Michael J.F. Barresi, Lara D. Hutson, Chi Bin Chien, Rolf O. Karlstrom
Biology Department Faculty Publication Series
Three major axon pathways cross the midline of the vertebrate forebrain early in embryonic development: the postoptic commissure (POC), the anterior commissure (AC) and the optic nerve. We show that a small population of Gfap+astroglia spans the midline of the zebrafish forebrain in the position of, and prior to, commissural and retinal axon crossing. These glial `bridges' form in regions devoid of the guidance molecules slit2 and slit3,although a subset of these glial cells express slit1a. We show that Hh signaling is required for commissure formation, glial bridge formation, and the restricted expression of the guidance molecules slit1a …