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Full-Text Articles in Life Sciences
Identification And Expression Analysis Of Methyl Jasmonate Responsive Ests In Paclitaxel Producing Taxus Cuspidata Suspension Culture Cells, Sangram K. Lenka, Nadia Boutaoui, Bibin Paulose, Kham Vongpaseuth, Jennifer Normanly, Susan C. Roberts, Elsbeth L. Walker
Identification And Expression Analysis Of Methyl Jasmonate Responsive Ests In Paclitaxel Producing Taxus Cuspidata Suspension Culture Cells, Sangram K. Lenka, Nadia Boutaoui, Bibin Paulose, Kham Vongpaseuth, Jennifer Normanly, Susan C. Roberts, Elsbeth L. Walker
Elsbeth Walker
Background Taxol(R)(paclitaxel) promotes microtubule assembly and stabilization and therefore is a potent chemotherapeutic agent against wide range of cancers. Methyl jasmonate (MJ) elicited Taxus cell cultures provide a sustainable option to meet the growing market demand for paclitaxel. Despite its increasing pharmaceutical importance, the molecular genetics of paclitaxel biosynthesis is not fully elucidated. This study focuses on identification of MJ responsive transcripts in cultured Taxus cells using PCR-based suppression subtractive hybridization (SSH) to identify genes involved in global pathway control. Results Six separate SSH cDNA libraries of paclitaxel-accumulating Taxus cuspidata P991 cell lines were constructed at three different post-elicitation time …
Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Elsbeth Walker, S. Lee, J. C. Chiecko, Y. Lee, M. L. Guerinot, G. An
Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Elsbeth Walker, S. Lee, J. C. Chiecko, Y. Lee, M. L. Guerinot, G. An
Elsbeth Walker
Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to β-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green fluorescent protein fusion was localized to the …
Mutations In Arabidopsis Yellow Stripe-Like1 And Yellow Stripe-Like3 Reveal Their Roles In Metal Ion Homeostasis And Loading Of Metal Ions In Seeds, Elsbeth Walker, B. M. Waters, H. H. Chu, R. J. Didonato, L. A. Roberts, R. B. Eisley, B. Lahner, D. E. Salt
Mutations In Arabidopsis Yellow Stripe-Like1 And Yellow Stripe-Like3 Reveal Their Roles In Metal Ion Homeostasis And Loading Of Metal Ions In Seeds, Elsbeth Walker, B. M. Waters, H. H. Chu, R. J. Didonato, L. A. Roberts, R. B. Eisley, B. Lahner, D. E. Salt
Elsbeth Walker
Here, we describe two members of the Arabidopsis (Arabidopsis thaliana) Yellow Stripe-Like (YSL) family, AtYSL1 and AtYSL3. The YSL1 and YSL3 proteins are members of the oligopeptide transporter family and are predicted to be integral membrane proteins. YSL1 and YSL3 are similar to the maize (Zea mays) YS1 phytosiderophore transporter (ZmYS1) and the AtYSL2 iron (Fe)-nicotianamine transporter, and are predicted to transport metal-nicotianamine complexes into cells. YSL1 and YSL3 mRNAs are expressed in both root and shoot tissues, and both are regulated in response to the Fe status of the plant. β-Glucuronidase reporter expression, driven by YSL1 and YSL3 promoters, …
Yellow Stripe1. Expanded Roles For The Maize Iron-Phytosiderophore Transporter, Elsbeth Walker, L. A. Roberts, A. J. Pierson, Z Panaviene
Yellow Stripe1. Expanded Roles For The Maize Iron-Phytosiderophore Transporter, Elsbeth Walker, L. A. Roberts, A. J. Pierson, Z Panaviene
Elsbeth Walker
Graminaceous monocots, including most of the world's staple grains (i.e. rice, corn, and wheat) use a chelation strategy (Strategy II) for primary acquisition of iron from the soil. Strategy II plants secrete phytosiderophores (PS), compounds of the mugineic acid family that form stable Fe(III) chelates in soil. Uptake of iron-PS chelates, which occurs through specific transporters at the root surface, thus represents the primary route of iron entry into Strategy II plants. The gene Yellow stripe1 (Ys1) encodes the Fe(III)-PS transporter of maize (Zea mays). Here the physiological functions performed by maize YS1 were further defined by examining the pattern …