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Functional Role Of Ppal And Potential For Moss In Industrial Applications., Susana Perez Martinez May 2022

Functional Role Of Ppal And Potential For Moss In Industrial Applications., Susana Perez Martinez

Electronic Theses and Dissertations

This dissertation is an examination and characterization of the functional roles of PPAL. PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) is a recently discovered gene. PPAL homologs are present in all plants and many animals, where its function is largely unknown. It is possible that PPAL could participate in prenylation processes since it shares similarity to the α subunits of known prenylation enzymes. Prenylation is a post-translational modification of proteins that involves the addition of a lipid moiety to proteins to facilitate membrane targeting and association and promote protein-protein interactions. Prenylation has important roles in plant growth and development, including …


The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running Sep 2021

The Effects Of Ppal-1 In Arabidopsis Gamete Development, Amanda J White, Susana Perez-Martinez, Mark P. Running

The Cardinal Edge

Prenylation is a type of post-translational modification in which a 15- or 20-carbon lipid is added to the carboxyl (C) terminus of the protein. Arabidopsis thaliana contains the PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, which encodes a protein with homology to the α-subunits of the three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations in PPAL, one of which is ppal-1, which contains a T-DNA insertion in the fourth intron. We have previously observed that self-fertilizing heterozygous ppal-1 plants produce progeny in which homozygous ppal-1 is underrepresented. This project attempts to ascertain …


The Effects Of Ppal-1 In Arabidopsis Gamete Development., Amanda White May 2020

The Effects Of Ppal-1 In Arabidopsis Gamete Development., Amanda White

College of Arts & Sciences Senior Honors Theses

Prenylation is a type of post-translational modification in which a 15- or 20-carbon lipid is added to the carboxyl (C) terminus of the protein. Arabidopsis thaliana contains the PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, which encodes a protein with homology to the α-subunits of the three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations in PPAL, one of which is ppal-1, which contains a T-DNA insertion in the fourth intron. We have previously observed that self-fertilizing heterozygous ppal-1 plants produce progeny in which homozygous ppal-1 is underrepresented. This project attempts to ascertain …


Mutant Drought Phenotype Investigation : A Look Into Effects Of Protein Prenylation On Water Utilization In Conditions Of Varying Soil Saturation And Gene Knockout., Casey Rasmussen Dec 2013

Mutant Drought Phenotype Investigation : A Look Into Effects Of Protein Prenylation On Water Utilization In Conditions Of Varying Soil Saturation And Gene Knockout., Casey Rasmussen

College of Arts & Sciences Senior Honors Theses

Plants respond to environmental stress, such as drought, with increases in levels of Abscisic Acid (ABA). This stress response allows greater survival rates but results in the plants producing lower seed yield and biomass. Certain mutant strains of the Arabidopsis plant have been identified with mutations in coding regions involved in the formation of the α and β subunits of PFT and PGGT-I genes involved in protein prenylation. We explored the possibility of these mutant strains to better preserve seed yield and plant biomass under drought stressing conditions in comparison to the wild type (Columbia) strain, based on the mutants’ …