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Full-Text Articles in Life Sciences
Intracellular Recording, Sensory Field Mapping, And Culturing Identified Neurons In The Leech, Hirudo Medicinalis, Josh Titlow, Zana R. Majeed, John G. Nicholls, Robin L. Cooper
Intracellular Recording, Sensory Field Mapping, And Culturing Identified Neurons In The Leech, Hirudo Medicinalis, Josh Titlow, Zana R. Majeed, John G. Nicholls, Robin L. Cooper
Biology Faculty Publications
The freshwater leech, Hirudo medicinalis, is a versatile model organism that has been used to address scientific questions in the fields of neurophysiology, neuroethology, and developmental biology. The goal of this report is to consolidate experimental techniques from the leech system into a single article that will be of use to physiologists with expertise in other nervous system preparations, or to biology students with little or no electrophysiology experience. We demonstrate how to dissect the leech for recording intracellularly from identified neural circuits in the ganglion. Next we show how individual cells of known function can be removed from …
Neural Circuit Recording From An Intact Cockroach Nervous System, Josh Titlow, Zana R. Majeed, H. Bernard Hartman, Ellen Burns, Robin L. Cooper
Neural Circuit Recording From An Intact Cockroach Nervous System, Josh Titlow, Zana R. Majeed, H. Bernard Hartman, Ellen Burns, Robin L. Cooper
Biology Faculty Publications
The cockroach ventral nerve cord preparation is a tractable system for neuroethology experiments, neural network modeling, and testing the physiological effects of insecticides. This article describes the scope of cockroach sensory modalities that can be used to assay how an insect nervous system responds to environmental perturbations. Emphasis here is on the escape behavior mediated by cerci to giant fiber transmission in Periplaneta americana. This in situ preparation requires only moderate dissecting skill and electrophysiological expertise to generate reproducible recordings of neuronal activity. Peptides or other chemical reagents can then be applied directly to the nervous system in solution …