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Characterization Of Ammonia Oxidizing Bacteria In An Activated Sludge Plant Using A Fluorescent In Situ Hybridization Method, Gülnur Coşkuner
Characterization Of Ammonia Oxidizing Bacteria In An Activated Sludge Plant Using A Fluorescent In Situ Hybridization Method, Gülnur Coşkuner
Turkish Journal of Biology
The spatial arrangement of functionally important ammonia oxidizing bacteria (AOB) in activated sludge flocs is largely unknown and activated sludge flocs are regarded as heterogeneous agglomerations of bacterial communities. This study aimed to determine both the location of AOB microcolonies within flocs and the size variation of these microcolonies based on their location. Fluorescent in situ hybridization (FISH) was used in combination with a confocal scanning laser microscope (CSLM) to achieve these goals. Grab samples taken from a full-scale plug flow wastewater treatment plant were examined with probes targeted to AOB b-Proteobacteria (Nso1225) and a genus specific probe targeted to …