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Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Turkish Journal of Biology
The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on …
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Turkish Journal of Biology
Solanum trilobatum L. İs one of the most valued medicinal plants of Indian Ayurveda and Siddha medicine. An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. Trilobatum was established for the first time. High frequency direct shoot regeneration (89%) was achieved in MS medium containing 2 mg/L 6-benzylaminopurine and 0.25 mg/L indole-3-acetic acid. The Agrobacterium strain EHA 105 harboring the binary vector pCAMBIA 1301, which contained hygromycin phosphotransferase (hpt) as a selectable marker gene and β-glucuronidase (gus) as a reporter gene, was used for the transformation. The factors that …
Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus
Development Of An Improved Rna Interference Vector System For Agrobacterium-Mediated Plant Transformation, Umut Toprak, Cathy Coutu, Doug Baldwin, Martin Erlandson, Dwayne Hegedus
Turkish Journal of Biology
Plant-mediated RNA interference (RNAi) has shown a great potential in pest control and requires i) subcloning of sense/antisense regions in compatible vectors, ii) transfer of the silencing cassette into a binary vector, iii) transformation of Agrobacterium tumefaciens with desired binary plasmids, and iv) transformation of plants with Agrobacterium. The procedure is long and should ensure plasmid backbone stability; however, plasmid recombination due to antibiotic selection is a common problem. pGSA1252 is an RNAi silencing binary vector allowing direct cloning of hairpin structure; however, it possesses a chloramphenicol selection marker leading to plasmid recombination in various Agrobacterium strains. To solve this …
Susceptibility Of Different Bean (Phaseolus Vulgaris L.) Cultivars To Agrobacterium Tumefaciens, Azi̇z Karakaya, Sebahatti̇n Özcan
Susceptibility Of Different Bean (Phaseolus Vulgaris L.) Cultivars To Agrobacterium Tumefaciens, Azi̇z Karakaya, Sebahatti̇n Özcan
Turkish Journal of Biology
Although several direct gene transfer techniques have been developed, Agrobacterium tumefaciens is still preferred for the transfer of foreign genes into many plant species. Perhaps the greatest weakness of A. tumefaciens is the host range limitation. Therefore, in this study, the susceptibility of different bean cultivars to oncogenic A. tumefaciens strain A281 was assessed. The cultivars 4F-2409, Karacaşehir-90, Akman-98 and Eskişehir-855 resulted in higher frequencies of tumor formation than the other cultivars tested. Tumor formation was not observed in Afyon-3, IVD-10, Göynük-98, Yunus-90 or Şehirali-90 bean cultivars. Tumor formation was observed on both the hypocotyl and epicotyl portions of the …
Susceptibility Of Chickpea ( Cicer Arietinuml.)Cultivars To Agrobacterium Tumefaciens(Smith Andtownsend) Conn, Azi̇z Karakaya, Sebahatti̇n Özcan
Susceptibility Of Chickpea ( Cicer Arietinuml.)Cultivars To Agrobacterium Tumefaciens(Smith Andtownsend) Conn, Azi̇z Karakaya, Sebahatti̇n Özcan
Turkish Journal of Biology
Twelve chickpea cultivars grown in Turkey were assessed for susceptibility to wild-type Agrobacterium tumefaciens strains A281 and A136NC. Although all chickpea cultivars showed susceptibility to infection from both A. tumefaciens strains, Diyar-95, Flip 87-8, Ispanyol, Gökçe, Damla-89, and Aziziye-94 had higher frequencies of tumor formation from both strains than the other cultivars tested.