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Full-Text Articles in Life Sciences
Adult And Regenerating Planarians Respond Differentially To Chronic Drug Exposure, Kevin Bayingana , '23, Danielle Ireland, Elizabeth Rosenthal , '23, Christina Rabeler, Eva-Maria S. Collins
Adult And Regenerating Planarians Respond Differentially To Chronic Drug Exposure, Kevin Bayingana , '23, Danielle Ireland, Elizabeth Rosenthal , '23, Christina Rabeler, Eva-Maria S. Collins
Biology Faculty Works
There is a lack of data on the effects of chronic exposure to common drugs and stimulants on the developing nervous system. Freshwater planarians have emerged as a useful invertebrate model amenable to high-throughput behavioral phenotyping to assay chemical safety in adult and developing brains. Here, we leverage the unique strength of the system to test in parallel for effects on the adult and developing nervous system, by screening ten common drugs and stimulants (forskolin, clenbuterol, LRE-1, MDL-12,330A, adenosine, caffeine, histamine, mianserin, fluoxetine and sertraline) using the asexual freshwater planarian Dugesia japonica. The compounds were tested up to 100 …
Differences In Neurotoxic Outcomes Of Organophosphorus Pesticides Revealed Via Multi-Dimensional Screening In Adult And Regenerating Planarians, Danielle Ireland, S. Zhang, Veronica Bochenek , ' 22, J.-H. Hsieh, Christina Rabeler, Zane Meyer , '21, Eva-Maria S. Collins
Differences In Neurotoxic Outcomes Of Organophosphorus Pesticides Revealed Via Multi-Dimensional Screening In Adult And Regenerating Planarians, Danielle Ireland, S. Zhang, Veronica Bochenek , ' 22, J.-H. Hsieh, Christina Rabeler, Zane Meyer , '21, Eva-Maria S. Collins
Biology Faculty Works
Organophosphorus pesticides (OPs) are a chemically diverse class of commonly used insecticides. Epidemiological studies suggest that low dose chronic prenatal and infant exposures can lead to life-long neurological damage and behavioral disorders. While inhibition of acetylcholinesterase (AChE) is the shared mechanism of acute OP neurotoxicity, OP-induced developmental neurotoxicity (DNT) can occur independently and/or in the absence of significant AChE inhibition, implying that OPs affect alternative targets. Moreover, different OPs can cause different adverse outcomes, suggesting that different OPs act through different mechanisms. These findings emphasize the importance of comparative studies of OP toxicity. Freshwater planarians are an invertebrate system that …
Dugesia Japonica Is The Best Suited Of Three Planarian Species For High-Throughput Toxicology Screening, D. Ireland, Veronica Bochenek , '22, Daniel Chaiken , '20, C. Rabeler, Sumi Onoe , '21, Ameet Soni, Eva-Maria S. Collins
Dugesia Japonica Is The Best Suited Of Three Planarian Species For High-Throughput Toxicology Screening, D. Ireland, Veronica Bochenek , '22, Daniel Chaiken , '20, C. Rabeler, Sumi Onoe , '21, Ameet Soni, Eva-Maria S. Collins
Biology Faculty Works
High-throughput screening (HTS) using new approach methods is revolutionizing toxicology. Asexual freshwater planarians are a promising invertebrate model for neurotoxicity HTS because their diverse behaviors can be used as quantitative readouts of neuronal function. Currently, three planarian species are commonly used in toxicology research: Dugesia japonica, Schmidtea mediterranea, and Girardia tigrina. However, only D. japonica has been demonstrated to be suitable for HTS. Here, we assess the two other species for HTS suitability by direct comparison with D. japonica. Through quantitative assessments of morphology and multiple behaviors, we assayed the effects of 4 common solvents (DMSO, …
Screening For Neurotoxic Potential Of 15 Flame Retardants Using Freshwater Planarians, S. Zhang, D. Ireland, N. S. Sipes, M. Behl, Eva-Maria S. Collins
Screening For Neurotoxic Potential Of 15 Flame Retardants Using Freshwater Planarians, S. Zhang, D. Ireland, N. S. Sipes, M. Behl, Eva-Maria S. Collins
Biology Faculty Works
Asexual freshwater planarians are an attractive invertebrate model for high-throughput neurotoxicity screening, because they possess multiple quantifiable behaviors to assess distinct neuronal functions. Planarians uniquely allow direct comparisons between developing and adult animals to distinguish developmentally selective effects from general neurotoxicity. In this study, we used our automated planarian screening platform to compare the neurotoxicity of 15 flame retardants (FRs), consisting of representative phased-out brominated (BFRs) and replacement organophosphorus FRs (OPFRs). OPFRs have emerged as a proposed safer alternative to BFRs; however, limited information is available on their health effects. We found 11 of the 15 FRs (3/6 BFRs, 7/8 …
Comparative Analysis Of Zebrafish And Planarian Model Systems For Developmental Neurotoxicity Screens Using An 87-Compound Library, D. Hagstrom, L. Truong, S. Zhang, R. Tanguay, Eva-Maria S. Collins
Comparative Analysis Of Zebrafish And Planarian Model Systems For Developmental Neurotoxicity Screens Using An 87-Compound Library, D. Hagstrom, L. Truong, S. Zhang, R. Tanguay, Eva-Maria S. Collins
Biology Faculty Works
There is a clear need to establish and validate new methodologies to more quickly and efficiently screen chemicals for potential toxic effects, particularly on development. The emergence of alternative animal systems for rapid toxicology screens presents valuable opportunities to evaluate how systems complement each other. In this article, we compare a chemical library of 87-compounds in two such systems, developing zebrafish and freshwater planarians, by screening for developmental neurotoxic effects. We show that the systems’ toxicological profiles are complementary to each other, with zebrafish yielding more detailed morphological endpoints and planarians more behavioral endpoints. Overall, zebrafish was more sensitive to …
Multi-Behavioral Endpoint Testing Of An 87-Chemical Compound Library In Freshwater Planarians, S. Zhang, D. Hagstrom, P. Hayes, A. Graham, Eva-Maria S. Collins
Multi-Behavioral Endpoint Testing Of An 87-Chemical Compound Library In Freshwater Planarians, S. Zhang, D. Hagstrom, P. Hayes, A. Graham, Eva-Maria S. Collins
Biology Faculty Works
There is an increased recognition in the field of toxicology of the value of medium-to-high-throughput screening methods using in vitro and alternative animal models. We have previously introduced the asexual freshwater planarian Dugesia japonica as a new alternative animal model and proposed that it is particularly well-suited for the study of developmental neurotoxicology. In this paper, we discuss how we have expanded and automated our screening methodology to allow for fast screening of multiple behavioral endpoints, developmental toxicity, and mortality. Using an 87-compound library provided by the National Toxicology Program (NTP), consisting of known and suspected neurotoxicants, including drugs, flame …