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Full-Text Articles in Life Sciences
The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky
The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky
Biology Faculty Works
Background: High temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.Results: The promoter of Hsp17.6 was used to build a Hsp17.6(p):GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p: GFP response dynamics. Hsp17.6(p):GFP signal increased with temperature increases from …
The Embryonic Transcriptome Of The Red-Eared Slider Turtle (Trachemys Scripta), Nicholas J. Kaplinsky, Scott F. Gilbert, J. Cebra-Thomas, K. Lilleväli, M. Saare, Eric Yanbo Chang , '13, Hannah Elizabeth Edelman , '12, Melissa Afton Frick , '12, Yin Guan , '13, Rebecca Ming Hammond , '13, Nicholas Henry Hampilos , '13, David Selassie Bampo Opoku , '12, Karim Sariahmed , '13, Eric Alan Sherman , '13, Ray Hess Bair Watson , '13
The Embryonic Transcriptome Of The Red-Eared Slider Turtle (Trachemys Scripta), Nicholas J. Kaplinsky, Scott F. Gilbert, J. Cebra-Thomas, K. Lilleväli, M. Saare, Eric Yanbo Chang , '13, Hannah Elizabeth Edelman , '12, Melissa Afton Frick , '12, Yin Guan , '13, Rebecca Ming Hammond , '13, Nicholas Henry Hampilos , '13, David Selassie Bampo Opoku , '12, Karim Sariahmed , '13, Eric Alan Sherman , '13, Ray Hess Bair Watson , '13
Biology Faculty Works
The bony shell of the turtle is an evolutionary novelty not found in any other group of animals, however, research into its formation has suggested that it has evolved through modification of conserved developmental mechanisms. Although these mechanisms have been extensively characterized in model organisms, the tools for characterizing them in non-model organisms such as turtles have been limited by a lack of genomic resources. We have used a next generation sequencing approach to generate and assemble a transcriptome from stage 14 and 17 Trachemys scripta embryos, stages during which important events in shell development are known to take place. …
Review Of "Translational Stem Cell Research: Issues Beyond The Debate On The Moral Status Of The Human Embryo" Edited By K. Hug And G. Hermerén, John B. Jenkins
Review Of "Translational Stem Cell Research: Issues Beyond The Debate On The Moral Status Of The Human Embryo" Edited By K. Hug And G. Hermerén, John B. Jenkins
Biology Faculty Works
No abstract provided.
Surprisingly Little Population Genetic Structure In A Fungus-Associated Beetle Despite Its Exploitation Of Multiple Hosts, Corlett W. Wood , '08, H. M. Donald, Vincent A. Formica, E. D. Brodie Iii
Surprisingly Little Population Genetic Structure In A Fungus-Associated Beetle Despite Its Exploitation Of Multiple Hosts, Corlett W. Wood , '08, H. M. Donald, Vincent A. Formica, E. D. Brodie Iii
Biology Faculty Works
In heterogeneous environments, landscape features directly affect the structure of genetic variation among populations by functioning as barriers to gene flow. Resource-associated population genetic structure, in which populations that use different resources (e.g., host plants) are genetically distinct, is a well-studied example of how environmental heterogeneity structures populations. However, the pattern that emerges in a given landscape should depend on its particular combination of resources. If resources constitute barriers to gene flow, population differentiation should be lowest in homogeneous landscapes, and highest where resources exist in equal proportions. In this study, we tested whether host community diversity affects population genetic …
Matrix Adhesion Polarizes Heart Progenitor Induction In The Invertebrate Chordate Ciona Intestinalis, J. Norton, J. Cooley, A. Islam, C. D. Cota, Bradley Justin Davidson , '91
Matrix Adhesion Polarizes Heart Progenitor Induction In The Invertebrate Chordate Ciona Intestinalis, J. Norton, J. Cooley, A. Islam, C. D. Cota, Bradley Justin Davidson , '91
Biology Faculty Works
Cell-matrix adhesion strongly influences developmental signaling. Resulting impacts on cell migration and tissue morphogenesis are well characterized. However, the in vivo impact of adhesion on fate induction remains ambiguous. Here, we employ the invertebrate chordate Ciona intestinalis to delineate an essential in vivo role for matrix adhesion in heart progenitor induction. In Ciona pre-cardiac founder cells, invasion of the underlying epidermis promotes localized induction of the heart progenitor lineage. We found that these epidermal invasions are associated with matrix adhesion along the pre-cardiac cell/epidermal boundary. Through targeted manipulations of RAP GTPase activity, we were able to manipulate pre-cardiac cell-matrix adhesion. …
Immobile Myosin-Ii Plays A Scaffolding Role During Cytokinesis In Budding Yeast, C. Wloka, Elizabeth Ann Vallen, Lydia Thé , '08, X. Fang, Y. Oh, E. Bi
Immobile Myosin-Ii Plays A Scaffolding Role During Cytokinesis In Budding Yeast, C. Wloka, Elizabeth Ann Vallen, Lydia Thé , '08, X. Fang, Y. Oh, E. Bi
Biology Faculty Works
Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized shortly before cytokinesis. This immobility was independent of actin filaments or the motor domain of Myo1 but required a small region in the Myo1 tail that is thought to be involved in higher-order assembly. As expected, proteins involved in actin ring assembly (tropomyosin and …