Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact With Phospholipase Dδ To Transduce Hydrogen Peroxide Signals In The Arabidopsis Response To Stress, Xuemin Wang, Liang Guo, Shivakumar P. Devaiah, Rama Narasimhan, Xiangqing Pan, Yanyan Zhang, Wenhua Zhang
Apr 2012
Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenases Interact With Phospholipase Dδ To Transduce Hydrogen Peroxide Signals In The Arabidopsis Response To Stress, Xuemin Wang, Liang Guo, Shivakumar P. Devaiah, Rama Narasimhan, Xiangqing Pan, Yanyan Zhang, Wenhua Zhang
Xuemin (Sam) Wang
Reactive oxygen species (ROS) are produced in plants under various stress conditions and serve as important mediators in plant responses to stresses. Here, we show that the cytosolic glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenases (GAPCs) interact with the plasma membrane–associated phospholipase D (PLDδ) to transduce the ROS hydrogen peroxide (H2O2) signal in Arabidopsis thaliana. Genetic ablation of PLDδ impeded stomatal response to abscisic acid (ABA) and H2O2, placing PLDδ downstream of H2O2 in mediating ABA-induced stomatal closure. To determine the molecular link between H2O2 and PLDδ, GAPC1 and GAPC2 were identified to bind to PLDδ, and the interaction was demonstrated by coprecipitation …
Levels Of Arabidopsis Thaliana Leaf Phosphatidic Acids, Phosphatidylserines, And Most Trienoate-Containing Polar Lipid Molecular Species Increase During The Dark Period Of The Diurnal Cycle, Xuemin Wang, Sara Maatta, Brad Scheu, Mary R. Roth, Pamela Tamura, Maoyin Li, Todd D. Williams, Ruth Welti
Mar 2012
Levels Of Arabidopsis Thaliana Leaf Phosphatidic Acids, Phosphatidylserines, And Most Trienoate-Containing Polar Lipid Molecular Species Increase During The Dark Period Of The Diurnal Cycle, Xuemin Wang, Sara Maatta, Brad Scheu, Mary R. Roth, Pamela Tamura, Maoyin Li, Todd D. Williams, Ruth Welti
Xuemin (Sam) Wang
Previous work has demonstrated that plant leaf polar lipid fatty acid composition varies during the diurnal (dark–light) cycle. Fatty acid synthesis occurs primarily during the light, but fatty acid desaturation continues in the absence of light, resulting in polyunsaturated fatty acids reaching their highest levels toward the end of the dark period. In this work, Arabidopsis thaliana were grown at constant (21°C) temperature with 12-h light and 12-h dark periods. Collision induced dissociation time-of-flight mass spectrometry (MS) demonstrated that 16:3 and 18:3 fatty acid content in membrane lipids of leaves are higher at the end of the dark than at …
Direct Infusion Mass Spectrometry Of Oxylipin-Containing Arabidopsis Membrane Lipids Reveals Varied Patterns In Different Stress Responses, Xuemin Wang, Hieu Sy Vu, Pamela Tamura, Nadezhda A. Galeva, Ratnesh Chaturvedi, Mary R. Roth, Todd Williams, Jyoti Shah, Ruth Welti
Dec 2011
Direct Infusion Mass Spectrometry Of Oxylipin-Containing Arabidopsis Membrane Lipids Reveals Varied Patterns In Different Stress Responses, Xuemin Wang, Hieu Sy Vu, Pamela Tamura, Nadezhda A. Galeva, Ratnesh Chaturvedi, Mary R. Roth, Todd Williams, Jyoti Shah, Ruth Welti
Xuemin (Sam) Wang
Direct infusion electrospray ionization triple quadrupole precursor scanning for three oxidized fatty acyl anions revealed 86 mass spectral peaks representing polar membrane lipids in extracts from Arabidopsis (Arabidopsis thaliana) infected with Pseudomonas syringae pv tomato DC3000 expressing AvrRpt2 (PstAvr). Quadrupole time-of-flight and Fourier transform ion cyclotron resonance mass spectrometry provided evidence for the presence of membrane lipids containing one or more oxidized acyl chains. The membrane lipids included molecular species of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, digalactosyldiacylglycerol, monogalactosyldiacylglycerol, and acylated monogalactosyldiacylglycerol. The oxidized chains were identified at the level of chemical formula and included C(18)H(27)O(3) (abbreviated 18:4-O, to indicate four double bond …
