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Evidence For And Characterization Of Ca2+ Binding To The Catalytic Region Of Arabidopsis Thaliana Phospholipase Dβ, Xuemin Wang
Evidence For And Characterization Of Ca2+ Binding To The Catalytic Region Of Arabidopsis Thaliana Phospholipase Dβ, Xuemin Wang
Xuemin (Sam) Wang
Most types of plant phospholipase D (PLD) require Ca2+ for activity, but how Ca2+ affects PLD activity is not well understood. We reported previously that Ca2+ binds to the regulatory C2 domain that occurs in the N terminus of the Ca2+-requiring PLDs. Using Arabidopsis thaliana PLDβ and C2-deleted PLDβ (PLDβcat), we now show that Ca2+ also interacts with the catalytic regions of PLD. PLDβcat exhibited Ca2+-dependent activity, was much less active, and required a higher level of Ca2+ than the full-length PLDβ. Ca2+ binding of the proteins was stimulated by phospholipids; phosphatidylserine was the most effective among those tested. Scatchard …