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Expression Profiling Of Arabidopsis Stigma Tissue Identifies Stigma-Specific Genes, Rob Swanson, T Clark, D Preuss
Expression Profiling Of Arabidopsis Stigma Tissue Identifies Stigma-Specific Genes, Rob Swanson, T Clark, D Preuss
Rob Swanson
Plants discriminate among pollen grains that land on the stigma surface, providing compatible pollen with the nutrients and signals required to proceed in pollination, and in many species, recognizing and inhibiting foreign pollen adhesion, hydration, germination and invasion. Much of the stigma machinery involved in these processes remains unknown. It is likely that the expression of a stigma-specific gene program confers specialized structural and functional properties. Here we used microarray technology and cDNA subtraction to build a profile of candidate stigma genes that facilitate early pollination events. Of over 24,000 Arabidopsis genes probed, we identified 11,403 genes expressed in stigma …
Callose (Β-1,3 Glucan) Is Essential For Arabidopsis Pollen Wall Patterning, But Not Tube Growth, Rob Swanson
Callose (Β-1,3 Glucan) Is Essential For Arabidopsis Pollen Wall Patterning, But Not Tube Growth, Rob Swanson
Rob Swanson
Background: Callose (β-1,3 glucan) separates developing pollen grains, preventing their underlying walls (exine) from fusing. The pollen tubes that transport sperm to female gametes also contain callose, both in their walls as well as in the plugs that segment growing tubes. Mutations in CalS5, one of several Arabidopsis β-1,3 glucan synthases, were previously shown to disrupt callose formation around developing microspores, causing aberrations in exine patterning, degeneration of developing microspores, and pollen sterility. Results: Here, we describe three additional cals5 alleles that similarly alter exine patterns, but instead produce fertile pollen. Moreover, one of these alleles (cals5-3) resulted in the …