Complete Genome Sequence Of Spiroplasma Citri Strain R8-A2 T , Causal Agent Of Stubborn Disease In Citrus Species, Robert E. Davis, Jonathan Shao, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, Nicole Donofrio
Apr 2017
Complete Genome Sequence Of Spiroplasma Citri Strain R8-A2 T , Causal Agent Of Stubborn Disease In Citrus Species, Robert E. Davis, Jonathan Shao, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, Nicole Donofrio
Gail Gasparich
Spiroplasma citri causes stubborn disease in Citrus spp. and diseases in other plants. Here, we report the nucleotide sequence of the 1,599,709-bp circular chromosome and two plasmids of S. citri strain R8-A2T. This information will facilitate analyses to understand spiroplasmal pathogenicity and evolutionary adaptations to lifestyles in plants and arthropod hosts.
Complete Genome Sequence Of Spiroplasma Sp. Nbrc 100390, Mindia Haryono, Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Mar 2017
Complete Genome Sequence Of Spiroplasma Sp. Nbrc 100390, Mindia Haryono, Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Gail Gasparich
Spiroplasma sp. NBRC 100390 was initially described as a duplicate of S. atrichopogonis GNAT3597T (ATCC BAA-520T) but later found to be different in the 16S rDNA sequences. Here, we report the complete genome sequence of this bacterium to establish its identity and to facilitate future investigation.
Complete Genome Sequence Of Spiroplasma Sp. Tu-14, Wen-Sui Lo, Mindia Haryono, Gail E. Gasparich, Chih-Horng Kuo
Jan 2017
Complete Genome Sequence Of Spiroplasma Sp. Tu-14, Wen-Sui Lo, Mindia Haryono, Gail E. Gasparich, Chih-Horng Kuo
Gail Gasparich
Spiroplasma sp. TU-14 was isolated from a contaminated sample of Entomoplasma lucivorax PIPN-2T obtained from the International Organization for Mycoplasmology collection. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma spp.
Complete Genome Sequence Of Spiroplasma Turonicum Tab4ct, A Bacterium Isolated From Horse Flies (Haematopota Sp.), Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Sep 2016
Complete Genome Sequence Of Spiroplasma Turonicum Tab4ct, A Bacterium Isolated From Horse Flies (Haematopota Sp.), Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Gail Gasparich
Spiroplasma turonicum Tab4cT was isolated from a horse fly (Haematopota sp.; probably Haematopota pluvialis) collected at Champchevrier, Indre-et-Loire, Touraine, France, in 1991. Here, we report the complete genome sequence of this bacterium to facilitate the investigation of its biology and the comparative genomics among Spiroplasma spp.
Complete Genome Sequence Of Spiroplasma Turonicum Strain Tab4ct, A Parasite Of A Horse Fly, Haematopota Sp. (Diptera: Tabanidae)., Robert E. Davis, Jonathan Shao, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, Nicole Donofrio
Nov 2015
Complete Genome Sequence Of Spiroplasma Turonicum Strain Tab4ct, A Parasite Of A Horse Fly, Haematopota Sp. (Diptera: Tabanidae)., Robert E. Davis, Jonathan Shao, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, Nicole Donofrio
Gail Gasparich
Spiroplasma turonicum was isolated from a Haematopota sp. fly in France. We report the nucleotide sequence of the circular chromosome of strain Tab4c(T). The genome information will facilitate evolutionary studies of spiroplasmas, including symbionts of insects and ticks and pathogens of plants, insects, crustaceans, and humans.
The Importance Of Terrestrial Dispersal For Connectivity Among Headwater Salamander Populations, William L. Miller, Joel W. Snodgrass, Gail E. Gasparich
Oct 2015
The Importance Of Terrestrial Dispersal For Connectivity Among Headwater Salamander Populations, William L. Miller, Joel W. Snodgrass, Gail E. Gasparich
Gail Gasparich
Many organisms are primarily constrained to the channels of the upstream terminus of river networks with limited capacities for both in‐stream and overland dispersal. Dispersal is believed to contribute to gene flow and the demographic stability of headwater populations. While assumed, the importance of overland dispersal to gene flow is largely unexplored in headwater salamanders. Six microsatellite markers were used to assess patterns of genetic population structure for a headwater salamander, Desmognathus fuscus. Tissue samples were collected using a nested hierarchical study design, which specifically addressed the contribution of overland movements to the genetic connectivity of headwater populations. Genetic …
Complete Genome Sequence Of Spiroplasma Kunkelii Strain Cr2-3x, Causal Agent Of Corn Stunt Disease In Zea Mays L., Robert E. Davis, Jonathan Shao, Ellen L. Dally, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, John C. Athey, Nigel A. Harrison, Nicole Donofrio
Oct 2015
Complete Genome Sequence Of Spiroplasma Kunkelii Strain Cr2-3x, Causal Agent Of Corn Stunt Disease In Zea Mays L., Robert E. Davis, Jonathan Shao, Ellen L. Dally, Yan Zhao, Gail E. Gasparich, Brady J. Gaynor, John C. Athey, Nigel A. Harrison, Nicole Donofrio
Gail Gasparich
Spiroplasma kunkelii causes corn stunt disease of Zea mays L. in the Americas. Here, we report the nucleotide sequence of the 1,463,926-bp circular chromosome and four plasmids of strain CR2-3x. This information will facilitate studies of Spiroplasma pathogenicity and evolutionary adaptations to transkingdom parasitism in plants and insect vectors.
Found And Lost: The Fates Of Horizontally Acquired Genes In Arthropod-Symbiotic Spiroplasma, Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Aug 2015
Found And Lost: The Fates Of Horizontally Acquired Genes In Arthropod-Symbiotic Spiroplasma, Wen-Sui Lo, Gail E. Gasparich, Chih-Horng Kuo
Gail Gasparich
Horizontal gene transfer (HGT) is an important mechanism that contributed to biological diversity, particularly in bacteria. Through acquisition of novel genes, the recipient cell may change its ecological preference and the process could promote speciation. In this study, we determined the complete genome sequence of two Spiroplasma species for comparative analyses and inferred the putative gene gains and losses. Although most Spiroplasma species are symbionts of terrestrial insects, Spiroplasma eriocheiris has evolved to be a lethal pathogen of freshwater crustaceans. We found that approximately 7% of the genes in this genome may have originated from HGT and these genes expanded …
Comparative Genome Analysis Of Spiroplasma Melliferum Ipmb4a, A Honeybee-Associated Bacterium, Wen-Sui Lo, Ling-Ling Chen, Wan-Chia Chung, Gail E. Gasparich, Chih-Horng Kuo
Dec 2012
Comparative Genome Analysis Of Spiroplasma Melliferum Ipmb4a, A Honeybee-Associated Bacterium, Wen-Sui Lo, Ling-Ling Chen, Wan-Chia Chung, Gail E. Gasparich, Chih-Horng Kuo
Gail Gasparich
Background: The genus Spiroplasma contains a group of helical, motile, and wall-less bacteria in the class
Mollicutes. Similar to other members of this class, such as the animal-pathogenic Mycoplasma and the
plant-pathogenic ‘Candidatus Phytoplasma’, all characterized Spiroplasma species were found to be associated with
eukaryotic hosts. While most of the Spiroplasma species appeared to be harmless commensals of insects, a small
number of species have evolved pathogenicity toward various arthropods and plants. In this study, we isolated a
novel strain of honeybee-associated S. melliferum and investigated its genetic composition and evolutionary history
by whole-genome shotgun sequencing and comparative analysis with …
Spiroplasma Eriocheiris Sp. Nov., Associated With Mortality In The Chinese Mitten Crab, Eriocheir Sinensis, Wen Wang, Wei Gu, Gail E. Gasparich, Keran Bi, Jiangtao Ou, Qingguo Meng, Tingming Liang, Qi Feng, Jianqiong Zhang, Ying Zhang
Mar 2011
Spiroplasma Eriocheiris Sp. Nov., Associated With Mortality In The Chinese Mitten Crab, Eriocheir Sinensis, Wen Wang, Wei Gu, Gail E. Gasparich, Keran Bi, Jiangtao Ou, Qingguo Meng, Tingming Liang, Qi Feng, Jianqiong Zhang, Ying Zhang
Gail Gasparich
A motile bacterium, designated strain TDA-040725-5T, was isolated from the haemolymph of a Chinese mitten crab, Eriocheir sinensis, with tremor disease. Based on 16S rRNA gene sequence analysis, the strain was phylogenetically distinct from other spiroplasmas but was closely related to Spiroplasma mirum ATCC 29335T. Cells of strain TDA-040725-5T were variable in length and shape, helical and motile, as determined by phase-contrast light microscopy. Examination by electron microscopy revealed wall-less cells delimited by a single membrane. The strain grew in M1D or R-2 liquid media at 20–40 °C, with optimum growth at 30 °C. Doubling time at the optimal temperature …
Absence Of Spiroplasma Or Other Bacterial 16s Rrna Genes In Brain Tissue Of Hamsters With Scrapie, Irina Alexeeva, Ellen J. Elliott, Sandra Rollins, Gail E. Gasparich, Jozef Lazar, Robert G. Rohwer
Dec 2005
Absence Of Spiroplasma Or Other Bacterial 16s Rrna Genes In Brain Tissue Of Hamsters With Scrapie, Irina Alexeeva, Ellen J. Elliott, Sandra Rollins, Gail E. Gasparich, Jozef Lazar, Robert G. Rohwer
Gail Gasparich
Spiroplasma spp. have been proposed to be the etiological agents of the transmissible spongiform encephalopathies (TSEs). In a blind study, a panel of 20 DNA samples was prepared from the brains of uninfected hamsters or hamsters infected with the 263K strain of scrapie. The brains of the infected hamsters contained ≥1010 infectious doses/g. The coded panel was searched for bacterial 16S rRNA gene sequences, using primers selective for spiroplasma sequences, primers selective for mollicutes in general, and universal bacterial primers. After 35 PCR cycles, no samples were positive for spiroplasma or any other bacterial DNA, while control Spiroplasma mirum genomic …
Spiroplasma Penaei Sp. Nov., Associated With Mortalities In Penaeus Vannamei, Pacific White Shrimp, Linda M. Nunan, Donald V. Lightner, Marietta A. Oduori, Gail E. Gasparich
Oct 2005
Spiroplasma Penaei Sp. Nov., Associated With Mortalities In Penaeus Vannamei, Pacific White Shrimp, Linda M. Nunan, Donald V. Lightner, Marietta A. Oduori, Gail E. Gasparich
Gail Gasparich
A new bacterial strain, designated SHRIMPT, isolated from the haemolymph of the Pacific white shrimp, Penaeus vannamei, was serologically distinct from other spiroplasmas. Cells of this strain were helical in form and variable in length. Examination by electron microscopy revealed wall-less cells delineated by a single cytoplasmic membrane. The organisms grew well in M1D media supplemented with 2 % NaCl. Strain SHRIMPT grew at temperatures of 20–37 °C, with optimum growth occurring at 28 °C. The strain catabolized glucose and hydrolysed arginine, but did not hydrolyse urea. The G+C content of the DNA was 29±1 mol%. Strain SHRIMPT (=ATCC BAA-1082T=CAIM …
Spiroplasma Leucomae Sp. Nov., Isolated In Poland From White Satin Moth (Leucoma Salicis L.) Larvae, Marietta A. Oduori, Jerzy J. Lipa, Gail E. Gasparich
Oct 2005
Spiroplasma Leucomae Sp. Nov., Isolated In Poland From White Satin Moth (Leucoma Salicis L.) Larvae, Marietta A. Oduori, Jerzy J. Lipa, Gail E. Gasparich
Gail Gasparich
Spiroplasma sp. strain SMAT, isolated in Poland from white satin moth larvae, Leucoma salicis L. (Lepidoptera: Lymantriidae), was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a single cell membrane (lacking a cell wall). Growth of strain SMAT occurred in M1D medium at 30 °C. Strain SMAT catabolized both glucose and arginine, but did not hydrolyse urea. The G+C content of the DNA was 24±1 mol% as determined by melting temperature analysis. Serological analysis revealed a very weak cross-reactivity …
Spiroplasma Atrichopogonis Sp. Nov., From A Ceratopogonid Biting Midge, R. Thomas Koerber, Gail E. Gasparich, Mark F. Frana, William L. Grogan
Dec 2004
Spiroplasma Atrichopogonis Sp. Nov., From A Ceratopogonid Biting Midge, R. Thomas Koerber, Gail E. Gasparich, Mark F. Frana, William L. Grogan
Gail Gasparich
Spiroplasma sp. strain GNAT3597T was isolated from the biting midge genus Atrichopogon (Diptera: Ceratopogonidae). It was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a cell membrane (i.e. lacking a cell wall). Growth of strain GNAT3597T occurred in M1D medium at 30 °C. Strain GNAT3597T catabolized both glucose and arginine, but did not hydrolyse urea. The DNA G+C content of strain GNAT3597T was 29±1 mol%. Only one strain, SMCAT (Spiroplasma mirum), is serologically related to strain GNAT3597T, although the …
A Spiroplasma Associated With Tremor Disease In The Chinese Mitten Crab (Eriocheir Sinensis), Wen Wang, Bohai Wen, Gail E. Gasparich, Ningning Zhu, Liwen Rong, Jianxiu Chen, Zaikuan Xu
Aug 2004
A Spiroplasma Associated With Tremor Disease In The Chinese Mitten Crab (Eriocheir Sinensis), Wen Wang, Bohai Wen, Gail E. Gasparich, Ningning Zhu, Liwen Rong, Jianxiu Chen, Zaikuan Xu
Gail Gasparich
An epidemic of tremor disease has been a serious problem in Chinese mitten crabs, Eriocheir sinensis, in China in recent years. The disease-causing agent was previously considered to be a rickettsia-like organism. Here, analysis of the 16S rRNA gene sequence, light and electron microscopy and cultivation in vitro were used to identify the agent. Sequence analysis of the 16S rRNA gene found it to have 98 % identity with that of Spiroplasma mirum. The agent was able to be passed through membrane filters with pores 220 nm in diameter and could be cultivated by inoculating the yolk sac of embryonated …
The Genus Spiroplasma And Its Non-Helical Descendants: Phylogenetic Classification, Correlation With Phenotype And Roots Of The Mycoplasma Mycoides Clade, Gail E. Gasparich, Robert F. Whitcomb, Deborah Dodge, Frank E. French, John Glass, David L. Williamson
Jan 2004
The Genus Spiroplasma And Its Non-Helical Descendants: Phylogenetic Classification, Correlation With Phenotype And Roots Of The Mycoplasma Mycoides Clade, Gail E. Gasparich, Robert F. Whitcomb, Deborah Dodge, Frank E. French, John Glass, David L. Williamson
Gail Gasparich
The genus Spiroplasma (helical mollicutes: Bacteria: Firmicutes: Mollicutes: Entomoplasmatales: Spiroplasmataceae) is associated primarily with insects. The Mycoplasma mycoides cluster (sensu Weisburg et al. 1989 and Johansson and Pettersson 2002 ) is a group of mollicutes that includes the type species – Mycoplasma mycoides – of Mycoplasmatales, Mycoplasmataceae and Mycoplasma. This cluster, associated solely with ruminants, contains five other species and subspecies. Earlier phylogenetic reconstructions based on partial 16S rDNA sequences and a limited sample of Spiroplasma and Mycoplasma sequences suggested that the genus Mycoplasma was polyphyletic, as the M. mycoides cluster and the grouping that consisted of the hominis and …
Spiroplasmas: Evolution, Adaptation And Diversity, Gail E. Gasparich
Feb 2002
Spiroplasmas: Evolution, Adaptation And Diversity, Gail E. Gasparich
Gail Gasparich
Since its designation as a separate genus some 30 years ago, Spiroplasmas have been well documented in a wide range of hosts and as the causative agent of several plant and insect diseases. One major area of research is the continued identification and taxonomical characterization of new Spiroplasma sp. combined with a determination of phylogenetic relationships among the various Spiroplasma sp. and between the Spiroplasmas and other members of the Mollicutes and Eubacteria. Although most phylogenetic analyses have been dependent on 16S rDNA sequence data, progress in two Spiroplasma sp. genome sequencing projects will provide new genomic regions for comparative …
Revised Group Classification Of The Genus Spiroplasma, David L. Williamson, Robert F. Whitcomb, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bové, Kevin J. Hackett, Jean R. Adams, Roberta B. Henegar, Meghnad Konai, Claude Chastel, Frank E. French
Dec 1997
Revised Group Classification Of The Genus Spiroplasma, David L. Williamson, Robert F. Whitcomb, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bové, Kevin J. Hackett, Jean R. Adams, Roberta B. Henegar, Meghnad Konai, Claude Chastel, Frank E. French
Gail Gasparich
Significant changes have been made in the systematics of the genus Spiroplasma (class Mollicutes) since it was expanded by revision in 1987 to include 23 groups and eight sub-groups. Since that time, two additional spiroplasmas have been assigned group numbers and species names. More recently, specific epithets have been assigned to nine previously designated groups and three sub-groups. Also, taxonomic descriptions and species names have been published for six previously ungrouped spiroplasmas. These six new organisms are: Spiroplasma alleghenense (strain PLHS-1T) (group XXVI), Spiroplasma lineolae (strain TALS-2T) (group XXVII), Spiroplasma platyhelix (strain PALS-1T) (group XXVIII), Spiroplasma montanense (strain HYOS-1T) (group …
Serologic And Genomic Relatedness Of Group Xiv Spiroplasma Isolates From A Lampyrid Beetle And Tabanid Flies: An Ecologic Paradox, G. E. Gasparich, K. J. Hackett, F. E. French, R. F. Whitcomb
Dec 1997
Serologic And Genomic Relatedness Of Group Xiv Spiroplasma Isolates From A Lampyrid Beetle And Tabanid Flies: An Ecologic Paradox, G. E. Gasparich, K. J. Hackett, F. E. French, R. F. Whitcomb
Gail Gasparich
Spiroplasma group XIV strain EC-1T and other isolates from the lampyrid beetle Ellychnia corrusca form a serogroup with tabanid spiroplasma strains (TC-1 and TS-1). It was hypothesized that similarities among these strains reflect a transmission cycle in which lampyrid beetles serve as overwintering hosts and tabanid adults become infected and transmit a homogeneous population of spiroplasma strains during spring, summer and autumn. In the present study, variations in restriction fragment length patterns suggest the presence of multiple genovars. Genotypic analysis may therefore be a companion to serology in elucidating spiroplasma diversity, and may provide clues to strain host range.
Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Sep 1997
Spiroplasma Lineolae Sp. Nov., From The Horsefly Tabanus Lineola (Diptera : Tabanidae), Frank E. French, Robert F. Whitcomb, Joseph G. Tully, Patricia Carle, Joseph M. Bové, Roberta B. Henegar, Jean R. Adams, Gail E. Gasparich, David L. Williamson
Gail Gasparich
Spiroplasma strain TALS-2T from the viscera of the striped horsefly, Tabanus lineola, collected in Georgia was serologically distinct from other Spiroplasma species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2T revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication …
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Jun 1997
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Gail Gasparich
Four spiroplasma strains, DF-1T, TG-1T, TABS-2T, and TAUS-1T, all of which were isolated from deerflies or horseflies (Diptera: Tabanidae), were serologically distinct from previously described spiroplasma species, groups, and subgroups. Strain DF-1Toriginated from a Maryland deerfly (Chrysops sp.); strain TG-1Twas isolated from a Maryland horsefly (Tabanus gladiator); strain TAUS-1Toriginated from a member of the Tabanus abdominalis-limbatinevris complex of horseflies collected in Maryland; and strain TABS-2Twas isolated from a horsefly (Tabanus abactor) collected in Oklahoma. Cells of all of the strains appeared to be helical and motile when they were examined by dark-field microscopy. Cells of strain DF-1Tgrowing in M1D medium …
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Dec 1996
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Gail Gasparich
The Colorado potato beetle, Leptinotarsa decemlineata, is a major holarctic pest of solanaceous crops. Presumably, this insect spread from Solanum species in central America to the Mexican plateau, and this was followed by multiple invasions of North America and Europe. Attempts are being made to control this beetle by using a genetically modified spiroplasma that occurs naturally in its gut. In the current study, spiroplasmas isolated from beetles collected in North America and Poland exhibited serologic (spiroplasma motility inhibition test) and genomic (restriction fragment length polymorphism) profiles that suggest that there were multiple spiroplasma introductions. Two serovars were identified; one …
Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Aug 1996
Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Gail Gasparich
In North America, the Colorado potato beetle, Leptinotarsa decemlineata, is often infected with the host-specific, gut-inhabiting Colorado potato beetle spiroplasma (CPBS). CPBS is apparently a commensal, but it may be useful in biocontrol if it can be transformed to express an insect-lethal gene. Difficulty in cultivating the organism, however, has hindered the development of a suitable transformation system. In this study, we eliminated the need for coculturing CPBS with insect cells. CPBS was reliably isolated with the BBL Anaerobic GasPak Jar system (low redox, enhanced CO(inf2)), which was easier to use and less expensive than insect cell coculture methods. A …
The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant
Aug 1992
The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant
Gail Gasparich
The 3' region of the cpc operon of Synechococcus sp. PCC 7002 has been sequenced, transcriptionally characterized, and analyzed by interposon mutagenesis. The cpc operon contains six genes, 5' cpcB-cpcA-cpcC-cpcD-cpcE-cpcF 3', and gives rise to at least eight (more likely ten) discrete mRNA transcripts. The steady-state levels of transcripts for the cpcE and cpcF genes are very low and are estimated to represent only about 1-2% of the total transcripts arising from the cpc locus. The cpcE gene predicts a protein of 268 amino acid residues, whereas the cpcF gene predicts a protein of 205 amino acid residues. The deduced …
Nucleotide Sequence And Further Characterization Of The Synechococcus Sp. Strain Pcc 7002 Reca Gene: Complementation Of A Cyanobacterial Reca Mutation By The Escherichia Coli Reca Gene, R C. Murphy, G E. Gasparich, D A. Bryant, R D. Porter
Jan 1990
Nucleotide Sequence And Further Characterization Of The Synechococcus Sp. Strain Pcc 7002 Reca Gene: Complementation Of A Cyanobacterial Reca Mutation By The Escherichia Coli Reca Gene, R C. Murphy, G E. Gasparich, D A. Bryant, R D. Porter
Gail Gasparich
The nucleotide sequence and transcript initiation site of the Synechococcus sp. strain PCC 7002 recA gene have been determined. The deduced amino acid sequence of the RecA protein of this cyanobacterium is 56% identical and 73% similar to the Escherichia coli RecA protein. Northern (RNA) blot analysis indicates that the Synechococcus strain PCC 7002 recA gene is transcribed as a monocistronic transcript 1,200 bases in length. The 5' endpoint of the recA mRNA was mapped by primer extension by using synthetic oligonucleotides of 17 and 27 nucleotides as primers. The nucleotide sequence 5' to the mapped endpoint contained sequence motifs …
