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Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna
Articles
The goal of this protocol is to allow for the rapid verification of bioinformatically identified terminators. Further, the plasmid (pGR-Blue) is designed specifically for this protocol and allows for the quantification of terminator efficiency. As a proof of concept, six terminators were bioinformatically identified in the mycobacteriophage Bernal13. Once identified, terminators were then made as oligonucleotides with the appropriate sticky ends and annealed together. Using Golden Gate Assembly (GGA), terminators were then cloned into pGR-Blue. Under visible light, false positive colonies appear blue and positively transformed colonies are white/yellow. After induction of an arabinose inducible promoter (pBad) with arabinose, colony …
Evaluation Of Marker-Assisted Introgression Of Yield Qtl Alleles Into Adapted Soybean, Nathan S. Reyna, C.H. Sneller
Evaluation Of Marker-Assisted Introgression Of Yield Qtl Alleles Into Adapted Soybean, Nathan S. Reyna, C.H. Sneller
Articles
Genetic diversity is limited in southern elite soybean [Glycine max (L.) Merrill]. Introgression of diverse alleles for yield may increase the rate of yield improvement. Beneficial yield alleles at three quantitative trait loci (QTL) from the northern cultivar Archer have been tagged with molecular markers. The objective of this research was to assess the value of the three Archer alleles for increased yield in southern environments and genetic backgrounds. Four sets of near isogenic lines (NIL) for each quantitative trait locus (QTL) were derived from heterozygous F6 plants identified from the crosses of Archer X Asgrow A5403 and …