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Simplifying Methods For In Vitro Metamorphosis Of Glochidia, Morgan A. Kern
Simplifying Methods For In Vitro Metamorphosis Of Glochidia, Morgan A. Kern
MSU Graduate Theses
Captive culture of Unionoid mussels is complicated by the parasitic larval stage, which normally requires a host fish for metamorphosis. Alternatively, some mussel species can metamorphose in vitro, i.e. in an artificial medium in Petri dishes. Most workers have used 5% CO2 atmosphere and bicarbonate to stabilize pH, requiring a specialized incubator. In the present study, in vitro metamorphosis success of Anodonta oregonensis and other species were higher or similar in air than in 1%, or 5% CO2. The nutritional role of the medium was tested by substituting physiological saline without nutrients at varying intervals before …