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Full-Text Articles in Life Sciences
Diversity Of Reptile Sex Chromosome Evolution Revealed By Cytogenetic And Linked-Read Sequencing, Ze-Xian Zhu, Kazumi Matsubara, Foyez Shams, Jason Dobry, Erik Wapstra, Tony Gamble, Stephen D. Sarre, Arthur Georges, Jennifer A. Marshall Graves, Qi Zhou, Tariq Ezaz
Diversity Of Reptile Sex Chromosome Evolution Revealed By Cytogenetic And Linked-Read Sequencing, Ze-Xian Zhu, Kazumi Matsubara, Foyez Shams, Jason Dobry, Erik Wapstra, Tony Gamble, Stephen D. Sarre, Arthur Georges, Jennifer A. Marshall Graves, Qi Zhou, Tariq Ezaz
Biological Sciences Faculty Research and Publications
Reptile sex determination is attracting much attention because the great diversity of sex-determination and dosage compensation mechanisms permits us to approach fundamental questions about mechanisms of sex chromosome turnover. Recent studies have made significant progress in better understanding diversity and conservation of reptile sex chromosomes, with however no reptile master sex determination genes identified. Here we describe an integrated genomics and cytogenetics pipeline, combining probes generated from the microdissected sex chromosomes with transcriptome and genome sequencing to explore the sex chromosome diversity in non-model Australian reptiles. We tested our pipeline on a turtle, two species of geckos, and a monitor …
A Chromosome-Level Genome Assembly Of The Parasitoid Wasp, Cotesia Glomerata (Hymenoptera: Braconidae), Brendan J. Pinto, Jerome J. Weis, Tony Gamble, Paul J. Ode, Ryan Paul, Jennifer M. Zaspel
A Chromosome-Level Genome Assembly Of The Parasitoid Wasp, Cotesia Glomerata (Hymenoptera: Braconidae), Brendan J. Pinto, Jerome J. Weis, Tony Gamble, Paul J. Ode, Ryan Paul, Jennifer M. Zaspel
Biological Sciences Faculty Research and Publications
Hymenopterans make up about 20% of all animal species, but most are poorly known and lack high-quality genomic resources. One group of important, yet understudied hymenopterans are parasitoid wasps in the family Braconidae. Among this understudied group is the genus Cotesia, a clade of ~1,000 species routinely used in studies of physiology, ecology, biological control, and genetics. However, our ability to understand these organisms has been hindered by a lack of genomic resources. We helped bridge this gap by generating a high-quality genome assembly for the parasitoid wasp, Cotesia glomerata (Braconidae; Microgastrinae). We generated this assembly using multiple sequencing …