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Lof1 And Interacting Transcription Factors In Plant Development And Crop Yield, Zoe Yeoh, Elizabeth Luscher, Patricia Springer
Lof1 And Interacting Transcription Factors In Plant Development And Crop Yield, Zoe Yeoh, Elizabeth Luscher, Patricia Springer
Student Publications
Transcription factors (TFs) help ensure proper gene expression in developing tissues, and thus play a role in plant development and plant architecture. LATERAL ORGAN FUSION1, or LOF1, is a TF expressed in the organ boundaries of Arabidopsis thaliana. lof1 mutants have fused axillary branches and cauline leaves, which indicates importance in boundary development. Because transcription factors are known to act in complexes, we wanted to discover what other proteins interact with LOF1. We executed a yeast-2-hybrid (Y2H) screen that identified several TFs as potential interactors: WHIRLY 3 (WHY3), MYB DOMAIN PROTEIN32 (MYB32), HOMEOBOX-LEUCINE ZIPPER PROTEIN4 (HB4), and LIGHT RESPONSE BTB2 …