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Full-Text Articles in Life Sciences
Molecular Approaches For Improving Desiccation Tolerance: Insights From The Brine Shrimp Artemia Franciscana, Steven C. Hand, Michael A. Menze
Molecular Approaches For Improving Desiccation Tolerance: Insights From The Brine Shrimp Artemia Franciscana, Steven C. Hand, Michael A. Menze
Faculty Research & Creative Activity
Main conclusion We have evaluated the endogenous expression and molecular properties of selected Group 3 LEA proteins from Artemia franciscana , and the capacity of selected Groups 1 and 3 proteins transfected into various desiccation-sensitive cell lines to improve tolerance to drying. Organisms inhabiting both aquatic and terrestrial ecosystems frequently are confronted with the problem of water loss for multiple reasons—exposure to hypersalinity, evaporative water loss, and restriction of intracellular water due to freezing of extracellular fluids. Seasonal desiccation can become severe and lead to the production of tolerant propagules and entry into the state of anhydrobiosis at various stages …
Diapause And Anhydrobiosis In Embryos Of Artemia Franciscana: Metabolic Depression, Lea Proteins And Water Stress, Steven C. Handel, Yuvraj Patil, Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael A. Menze, Leaf C. Boswell, Daniel Moore, Mehmet Toner
Diapause And Anhydrobiosis In Embryos Of Artemia Franciscana: Metabolic Depression, Lea Proteins And Water Stress, Steven C. Handel, Yuvraj Patil, Shumin Li, Nilay Chakraborty, Apurva Borcar, Michael A. Menze, Leaf C. Boswell, Daniel Moore, Mehmet Toner
Faculty Research & Creative Activity
Metabolic depression is typically correlated with extended survival of environmental challenge and energy-limitation in early life stages of various invertebrates and vertebrates. Diapause is an ontogenetically-programmed reduction of development and often metabolism seen in many invertebrates. When embryos of Artemia franciscana enter the state of diapause, the overall metabolic depression is estimated to be greater than 99%. These embryos also contain trehalose and express multiple isoforms of Late Embryogenesis Abundant (LEA) proteins, constituents often present in a number of such anhydrobiotic animals. The mRNA levels for LEA proteins are highest in diapause and post-diapause embryos that possess desiccation tolerance, but …
Trehalose Transporter From African Chironomid Larvae Improves Desiccation Tolerance Of Chinese Hamster Ovary Cells, Nilay Chakraborty, Michael A. Menze, Heidi Elmoazzen, Halong Vu, Martin Yarmush, Steven C. Hand, Mehmet Toner
Trehalose Transporter From African Chironomid Larvae Improves Desiccation Tolerance Of Chinese Hamster Ovary Cells, Nilay Chakraborty, Michael A. Menze, Heidi Elmoazzen, Halong Vu, Martin Yarmush, Steven C. Hand, Mehmet Toner
Faculty Research & Creative Activity
Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disaccharide trehalose. Trehalose is known to improve desiccation tolerance in mammalian cells when present on both sides of the cell membrane. Because trehalose is membrane impermeant the development of desiccation strategies involving this promising sugar is hindered. We explored the potential of using a high-capacity trehalose transporter (TRET1) from the African chironomid Polypedilum vanderplanki[21] …
Lea Proteins During Water Stress: Not Just For Plants Anymore, Steven C. Hand, Michael A. Menze, Mehmet Toner, Leaf Boswell, Daniel Moore
Lea Proteins During Water Stress: Not Just For Plants Anymore, Steven C. Hand, Michael A. Menze, Mehmet Toner, Leaf Boswell, Daniel Moore
Faculty Research & Creative Activity
Late embryogenesis abundant (LEA) proteins are extremely hydrophilic proteins that were first identified in land plants. Intracellular accumulation is tightly correlated with acquisition of desiccation tolerance, and data support their capacity to stabilize other proteins and membranes during drying, especially in the presence of sugars like trehalose. Exciting reports now show LEA proteins are not restricted to plants; multiple forms are expressed in desiccation-tolerant animals from at least four phyla. We evaluate here the expression, subcellular localization, biochemical properties and potential functions of LEA proteins in animal species during water stress. LEA proteins are intrinsically unstructured in aqueous solution, but …