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Structural And Functional Studies Of The Tryptophan Operon Of Bacillus Pumilus, Miriam V. Rivas
Structural And Functional Studies Of The Tryptophan Operon Of Bacillus Pumilus, Miriam V. Rivas
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The nucleotide sequence of the trpE, trpD, and 5’trpC genes of the Bacillus pumilus tryptophan operon was determined for a comparative evaluation. DNA comparisons to the tryptophan operon of a related species, Bacillus subtilis, showed 66% base homology. The overlapping of translational start and stop signals indicate that, similar to Escherichia coli, the trpE, trpD, and trpC genes of the B.pumilus trp operon are translationally coupled. The values for amino acid identity between the two Bacilli showed 68, 62, and 65% for trpE, trpD and 5’trpC genes, respectively. Charge related residues …