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Biology

California Polytechnic State University, San Luis Obispo

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Proteomics

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Full-Text Articles in Life Sciences

Are Circadian Cycles The Dominant Proteome Rhythym In The Intertidal Mussel Mytilus Californianus?, Kristina M. Koster, Cory Elowe, Lars Tomanek Jan 2015

Are Circadian Cycles The Dominant Proteome Rhythym In The Intertidal Mussel Mytilus Californianus?, Kristina M. Koster, Cory Elowe, Lars Tomanek

STAR Program Research Presentations

Mytilus californianus, also known as the California mussel, is a marine bivalve that is abundant along the West coast from Alaska to southern Baja California. They mainly reside in the upper-middle intertidal zone and cling to pier pilings and surf exposed rocks. They create multi-layered beds, which form a habitat for algae and many species of invertebrates. Intertidal mussels live in a naturally dynamic environment. It has previously been reported (Connor and Gracey, 2011) that the 24-hour circadian (day to night) rhythm of the intertidal mussel Mytilus californianus is primarily responsible for its rhythmic gene expression, as opposed to …


The Proteomic Response Of Sea Squirts (Genus Ciona) To Acute Heat Stress: A Global Perspective On The Thermal Stability Of Proteins, Loredana Serafini, Jay B. Hann, Dietmar Kültz, Lars Tomanek Sep 2011

The Proteomic Response Of Sea Squirts (Genus Ciona) To Acute Heat Stress: A Global Perspective On The Thermal Stability Of Proteins, Loredana Serafini, Jay B. Hann, Dietmar Kültz, Lars Tomanek

Biological Sciences

Congeners belonging to the genus Ciona have disparate distributions limited by temperature. Ciona intestinalis is more widespread with a cosmopolitan distribution ranging from tropical to sub-arctic zones, while Ciona savignyi is limited to temperate-latitudes of the northern Pacific Ocean. To compare the heat stress response between congeners, we quantified changes in protein expression using proteomics. Animals were exposed to 22 °C, 25 °C, and 28 °C for 6 h, then recovered at a control temperature (13 °C) for 16 h (high heat stress experiment). In a second experiment we exposed animals to lower levels of heat stress at 18 °C, …