Life Sciences Commons^™

Acclimation And Hardening Of A Slow-Growing Woody Species Emblematic To Western North America From In Vitro Plantlets, Peggy Martinez, Marcelo Serpe, Rachael Barron, Sven Buerki

Biology Faculty Publications and Presentations

Premise: Determining the tolerance of plant populations to climate change requires the development of biotechnological protocols producing genetically identical individuals used for genotype-by-environment experiments. Such protocols are missing for slow-growth, woody plants; to address this gap, this study uses Artemisia tridentata, a western North American keystone shrub, as model.

Methods and Results: The production of individual lines is a two-step process: in vitro propagation under aseptic conditions followed by ex vitro acclimation and hardening. Due to aseptic growth conditions, in vitro plantlets exhibit maladapted phenotypes, and this protocol focuses on presenting an approach promoting morphogenesis for slow-growth, woody species. …