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Full-Text Articles in Life Sciences
Rna Sequence To Structure Analysis From Comprehensive Pairwise Mutagenesis Of Multiple Self-Cleaving Ribozymes, Jessica M. Roberts, James D. Beck, Tanner B. Pollock, Devin P. Bendixsen, Eric J. Hayden
Rna Sequence To Structure Analysis From Comprehensive Pairwise Mutagenesis Of Multiple Self-Cleaving Ribozymes, Jessica M. Roberts, James D. Beck, Tanner B. Pollock, Devin P. Bendixsen, Eric J. Hayden
Biology Faculty Publications and Presentations
Self-cleaving ribozymes are RNA molecules that catalyze the cleavage of their own phosphodiester backbones. These ribozymes are found in all domains of life and are also a tool for biotechnical and synthetic biology applications. Self-cleaving ribozymes are also an important model of sequence-to-function relationships for RNA because their small size simplifies synthesis of genetic variants and self-cleaving activity is an accessible readout of the functional consequence of the mutation. Here, we used a high-throughput experimental approach to determine the relative activity for every possible single and double mutant of five self-cleaving ribozymes. From this data, we comprehensively identified non-additive effects …
Intramolecular Phenotypic Capacitance In A Modular Rna Molecule, Eric J. Hayden, Devin P. Bendixsen, Andreas Wagner
Intramolecular Phenotypic Capacitance In A Modular Rna Molecule, Eric J. Hayden, Devin P. Bendixsen, Andreas Wagner
Biology Faculty Publications and Presentations
Phenotypic capacitance refers to the ability of a genome to accumulate mutations that are conditionally hidden and only reveal phenotype-altering effects after certain environmental or genetic changes. Capacitance has important implications for the evolution of novel forms and functions, but experimentally studied mechanisms behind capacitance are mostly limited to complex, multicomponent systems often involving several interacting protein molecules. Here we demonstrate phenotypic capacitance within a much simpler system, an individual RNA molecule with catalytic activity (ribozyme). This naturally occurring RNA molecule has a modular structure, where a scaffold module acts as an intramolecular chaperone that facilitates folding of a second …