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Full-Text Articles in Life Sciences
A Repeated Triple Lysine Motif Anchors Complexes Containing Bone Sialoprotein And The Type Xi Collagen A1 Chain Involved In Bone Mineralization, Jeff P. Gorski, Nichole T. Franz, Daniel Pernoud, Andrew Keightley, David R. Eyre, Julia Thom Oxford
A Repeated Triple Lysine Motif Anchors Complexes Containing Bone Sialoprotein And The Type Xi Collagen A1 Chain Involved In Bone Mineralization, Jeff P. Gorski, Nichole T. Franz, Daniel Pernoud, Andrew Keightley, David R. Eyre, Julia Thom Oxford
Biology Faculty Publications and Presentations
While details remain unclear, initiation of woven bone mineralization is believed to be mediated by collagen and potentially nucleated by bone sialoprotein (BSP). Interestingly, our recent publication showed that BSP and type XI collagen form complexes in mineralizing osteoblastic cultures. To learn more, we examined the protein composition of extracellular sites of de novo hydroxyapatite deposition which were enriched in BSP and Col11a1 containing an alternatively spliced “6b” exonal sequence. An alternate splice variant “6a” sequence was not similarly co-localized. BSP and Col11a1 co-purify upon ion-exchange chromatography or immunoprecipitation. Binding of the Col11a1 “6b” exonal sequence to bone sialoprotein was …