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Full-Text Articles in Life Sciences

Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler Mar 2024

Novel Microbiological Medium Developed For The Isolation Of Bacteria Associated With Estuarine Anemones, Parker K. Lund, Catalina Cuellar-Gempeler

IdeaFest: Interdisciplinary Journal of Creative Works and Research from Cal Poly Humboldt

Out of the nearly one trillion species of microbiota estimated to inhabit Earth only ten thousand have been cultured in the laboratory. Culturing continues to play a vital role in determining the physiology and ecologic function of individual bacteria in microbial communities and for microbes associated with host organisms one of the major challenges is developing microbiological media that mimics the bacteria’s natural environment enough to promote growth. Here, we target bacteria associated with the estuary anemones Diadumene lineata and Metridium senile by developing a novel medium that uses anemone tissue as the sole source of nutrients. We further measured …


First Recorded Occurrence Of The Parasitic Barnacle (Anelasma Squalicola) On A Greenland Shark (Somniosus Microcephalus) In The Canadian Arctic, Eric Ste-Marie, Henrik Glenner, David J. Rees, Nigel E. Hussey Jul 2023

First Recorded Occurrence Of The Parasitic Barnacle (Anelasma Squalicola) On A Greenland Shark (Somniosus Microcephalus) In The Canadian Arctic, Eric Ste-Marie, Henrik Glenner, David J. Rees, Nigel E. Hussey

Integrative Biology Publications

A solitary Anelasma squalicola specimen was collected from the cloaca of a Greenland shark (Somniosus microcephalus), the first time this association has been recorded. The specimen's identity was confirmed through morphological and genetic assessment (mitochondrial markers: COI and control region). A. squalicola is a species typically associated with deep-sea lantern sharks (Etmopteridae) and, until the present observation, had never been observed at a sexually mature size in the absence of a mating partner. Given the reported negative effects of this parasite on its hosts, monitoring Greenland sharks for additional cases is recommended.


Diverse Marine T4-Like Cyanophage Communities Are Primarily Comprised Of Low-Abundance Species Including Species With Distinct Seasonal, Persistent, Occasional, Or Sporadic Dynamics, Emily Dart, Jed A. Fuhrman, Nathan A. Ahlgren Feb 2023

Diverse Marine T4-Like Cyanophage Communities Are Primarily Comprised Of Low-Abundance Species Including Species With Distinct Seasonal, Persistent, Occasional, Or Sporadic Dynamics, Emily Dart, Jed A. Fuhrman, Nathan A. Ahlgren

Biology

Cyanophages exert important top-down controls on their cyanobacteria hosts; however, concurrent analysis of both phage and host populations is needed to better assess phage-host interaction models. We analyzed picocyanobacteria Prochlorococcus and Synechococcus and T4-like cyanophage communities in Pacific Ocean surface waters using five years of monthly viral and cellular fraction metagenomes. Cyanophage communities contained thousands of mostly low-abundance (<2% relative abundance) species with varying temporal dynamics, categorized as seasonally recurring or non-seasonal and occurring persistently, occasionally, or sporadically (detected in ≥85%, 15-85%, or <15% of samples, respectively). Viromes contained mostly seasonal and persistent phages (~40% each), while cellular fraction metagenomes had mostly sporadic species (~50%), reflecting that these sample sets capture different steps of the infection cycle-virions from prior infections or within currently infected cells, respectively. Two groups of seasonal phages correlated to Synechococcus or Prochlorococcus were abundant in spring/summer or fall/winter, respectively. Cyanophages likely have a strong influence on the host community structure, as their communities explained up to 32% of host community variation. These results support how both seasonally recurrent and apparent stochastic processes, likely determined by host availability and different host-range strategies among phages, are critical to phage-host interactions and dynamics, consistent with both the Kill-the-Winner and the Bank models.


The Toxicity Of Dopamine On Salish Sea Phytoplankton, Allyson Lombardo Jan 2023

The Toxicity Of Dopamine On Salish Sea Phytoplankton, Allyson Lombardo

WWU Graduate School Collection

In the Salish Sea, blooms of the intertidal macroalgae, Ulvaria obscura, are common and can achieve extraordinarily high biomass. Upon desiccation and subsequent rehydration from incoming tides, U. obscura releases dopamine. Previous studies showed that dopamine negatively affects other macroalgal species and can deter grazers. However, the effects of dopamine on co-occurring phytoplankton remains unknown. This study explored the toxicity of dopamine on four phytoplankton known to inhabit the Salish Sea: the haptophyte, Isochrysis galbana; the chlorophyte, Dunaliella tertiolecta; the dinoflagellate, Heterocapsa triquetra; and the diatom, Thalassiosira sp. Over the course of 8 days, phytoplankton growth …


Impacts Of Algal Morphology And Water Flow On Macroalgal Microplastic Capture, Cheyenne M. Adams Nov 2022

Impacts Of Algal Morphology And Water Flow On Macroalgal Microplastic Capture, Cheyenne M. Adams

Honors College Theses

Microplastic pollution is a major area of concern in marine environments, especially as microplastics enter the food web. This study used pipe cleaners and two lichen species as algal mimics, and Chaetomorpha sp. and Chondrus crispus as model algal species to test the effects of morphology and biomass on microbead and foam capture. This study also utilized two different water flow methods: vortices and waves. Results suggest that water flow, as well as biomass and morphology, play a role in microplastic capture in macroalgae. For all mimics and algal species, except Cladonia lichens, turfs with increased biomass and length showed …


Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven Mar 2022

Quantitative Assessment Of The Trophic Ecology Of The Oceanic Ctenophore, Bolinopsis Infundibulum, In Monterey Bay, California, Victoria C. Scriven

USF Tampa Graduate Theses and Dissertations

Bolinopsis infundibulum is a wide-ranging, ubiquitous ctenophore whose fragile nature makes the collection of specimens and quantification of key predator-prey activities in controlled laboratory experiments, challenging. Thus, in situ methods often represent the best means for data collection. However, while present in surface waters, these animals can also be abundant at depths well beyond those attainable by divers. As a result, very little empirical data exist over the depth range of their natural habitats which limits our ability to assess key predator-prey interactions needed to assess their ecological role in midwater food webs. Working in Monterey Bay, California, remotely operated …


Reply To Comment: Controls On Turnover Of Marine Dissolved Organic Matter-Testing The Null Hypothesis Of Purely Concentration-Driven Uptake, Yuan Shen, Ronald Benner Jan 2022

Reply To Comment: Controls On Turnover Of Marine Dissolved Organic Matter-Testing The Null Hypothesis Of Purely Concentration-Driven Uptake, Yuan Shen, Ronald Benner

Faculty Publications

Our recent bioassay experiments indicate that molecular properties are a primary control on the microbial utilization of dissolved organic matter in the ocean. This finding is questioned by Lennartz and Dittmar who modeled our experiments and concluded that our observations could be largely explained by concentration-driven uptake independent from molecular properties. We suggest the authors' models are deficient for establishing the relative roles of molecular properties and concentration-driven uptake. Our conclusion is consistent with earlier and recent experimental results and biogeochemical observations, supporting a unified theory with molecular properties as a more prominent control than concentration-driven uptake on marine organic …


Final Report Floating Upwelling System Harvest Road Oceans, Robert Michael, Scott Bennett Jan 2022

Final Report Floating Upwelling System Harvest Road Oceans, Robert Michael, Scott Bennett

Fisheries occasional publications

A Floating Upwelling System or FLUPSY is a mechanical system for the culture of seed stock during the nursery stage of commercial bivalve production.


Effects Of Invasional Meltdown On Community Structure In Marine Ecosystems In The Damariscotta Estuary Of Maine, Kate Lazzeri Jan 2021

Effects Of Invasional Meltdown On Community Structure In Marine Ecosystems In The Damariscotta Estuary Of Maine, Kate Lazzeri

Student Research Poster Presentations 2021

Basibiont organisms form the foundation of marine ecosystems by providing additional space for new species to settle on as epibionts. Invasive epibionts may take advantage of this new basibiont presence, which leads to many harmful effects on native organisms such as competition for both resources and food. In some cases, invasive species facilitate recruitment of other invasive organisms, a phenomenon referred to as invasional meltdown, but it is not known if invasion of new basibionts increases invasive epibiont occurrence. The purpose of this study is to answer the following research questions: 1) does the invasion status of the basibiont alter …


When Will Taxonomic Saturation Be Achieved? A Case Study In Nunduva And Kyrtuthrix (Rivulariaceae, Cyanobacteria)1, Jeffrey R. Johansen, Laura Gonzalez-Resendiz, Viviana Escobar-Sanchez, Claudia Segal-Kischinevzky, Jose Martınez-Yerena, Joaquin Hernandez-Sanchez, Gariela Hernandez-Perez, Hilda Leon-Tejera Jan 2021

When Will Taxonomic Saturation Be Achieved? A Case Study In Nunduva And Kyrtuthrix (Rivulariaceae, Cyanobacteria)1, Jeffrey R. Johansen, Laura Gonzalez-Resendiz, Viviana Escobar-Sanchez, Claudia Segal-Kischinevzky, Jose Martınez-Yerena, Joaquin Hernandez-Sanchez, Gariela Hernandez-Perez, Hilda Leon-Tejera

2021 Faculty Bibliography

A number of heterocytous, mat-forming, tapering cyanobacteria in Rivulariaceae have recently been observed in both the Atlantic and Pacific coasts in the rocky intertidal and supratidal zones. These belong to the genera Nunduva, Kyrtuthrix, and Phyllonema and have been the subject of several recent studies. Herein, two new species of Nunduva (N. komarkovae and N. sanagustinensis) and two new species of Kyrtuthrix (K. munecosensis and K. totonaca) are characterized and described from the coasts of Mexico. Genetic separation based on the 16S-23S ITS region was pronounced (>10% in all comparisons). Morphological differences between all existing species in these two …


Global Economic Costs Of Aquatic Invasive Alien Species, Ross N. Cuthbert, Zarah Pattison, Nigel G. Taylor, Laura Verbrugge, Christophe Diagne, Danish A. Ahmed, Boris Leroy, Elena Angulo, Elizabeta Briski, César Capinha, Jane A. Catford, Tatenda Dalu, Franz Essl, Rodolphe E. Gozlan, Phillip J. Haubrock, Melina Kourantidou, Andrew M. Kramer, David Renault, Ryan J. Wasserman, Franck Courchamp Jan 2021

Global Economic Costs Of Aquatic Invasive Alien Species, Ross N. Cuthbert, Zarah Pattison, Nigel G. Taylor, Laura Verbrugge, Christophe Diagne, Danish A. Ahmed, Boris Leroy, Elena Angulo, Elizabeta Briski, César Capinha, Jane A. Catford, Tatenda Dalu, Franz Essl, Rodolphe E. Gozlan, Phillip J. Haubrock, Melina Kourantidou, Andrew M. Kramer, David Renault, Ryan J. Wasserman, Franck Courchamp

Integrative Biology Faculty and Staff Publications

Much research effort has been invested in understanding ecological impacts of invasive alien species (IAS) across ecosystems and taxonomic groups, but empirical studies about economic effects lack synthesis. Using a comprehensive global database, we determine patterns and trends in economic costs of aquatic IAS by examining: (i) the distribution of these costs across taxa, geographic regions and cost types; (ii) the temporal dynamics of global costs; and (iii) knowledge gaps, especially compared to terrestrial IAS. Based on the costs recorded from the existing literature, the global cost of aquatic IAS conservatively summed to US$345 …


Assay Development For Isolation And Characterization Of Anticancer Properties Of Marine Fungi, Kyra Ricci Apr 2020

Assay Development For Isolation And Characterization Of Anticancer Properties Of Marine Fungi, Kyra Ricci

Honors Theses

Metabolites from fungi have potential use in the drug discovery process and have been used in the past to develop therapeutic agents for human use. Initial characterization of potential therapeutic properties of fungi is thus an important first step in identifying novel therapeutic compounds. In the present study, marine fungi were isolated from Myrtle Beach and assayed for anticancer properties using the soft agar colony formation assay in a 3D in vitro cellular environment. Many species were isolated from environmental samples, displaying unique morphologies and growth patterns. To optimize the soft agar assay in a six-well cell culture plate for …


Metatranscriptomic And Metagenomic Analysis Of Biological Diversity In Subglacial Lake Vostok (Antarctica), Colby Gura, Scott O. Rogers Mar 2020

Metatranscriptomic And Metagenomic Analysis Of Biological Diversity In Subglacial Lake Vostok (Antarctica), Colby Gura, Scott O. Rogers

Biological Sciences Faculty Publications

A combined metatranscriptomic and metagenomic study of Vostok (Antarctica) ice core sections from glacial, basal, and lake water accretion ice yielded sequences that indicated a wide variety of species and possible conditions at the base of the glacier and in subglacial Lake Vostok. Few organisms were in common among the basal ice and accretion ice samples, suggesting little transmission of viable organisms from the basal ice meltwater into the lake water. Additionally, samples of accretion ice, each of which originated from water in several locations of the shallow embayment, exhibit only small amounts of mixing of species. The western-most portion …


Marine Aquarium Fish Resource Of Western Australia Harvest Strategy : 2018 – 2022 : Version 1.0, Department Of Fisheries Sep 2018

Marine Aquarium Fish Resource Of Western Australia Harvest Strategy : 2018 – 2022 : Version 1.0, Department Of Fisheries

Fisheries management papers

No abstract provided.


Nunduva, A New Marine Genus Of Rivulariaceae (Nostocales, Cyanobacteria) From Marine Rocky Shores, Laura Gonzales-Resendiz, Jeffrey R. Johansen, Luisa Alba-Lois, Claudia Segal–Kischinevzky, Viviana Escobar–Sánchez, Luis F. Jimenez Garcia4, Tomáš Hauer, Hilda León–Tejera Jan 2018

Nunduva, A New Marine Genus Of Rivulariaceae (Nostocales, Cyanobacteria) From Marine Rocky Shores, Laura Gonzales-Resendiz, Jeffrey R. Johansen, Luisa Alba-Lois, Claudia Segal–Kischinevzky, Viviana Escobar–Sánchez, Luis F. Jimenez Garcia4, Tomáš Hauer, Hilda León–Tejera

2018 Faculty Bibliography

Several populations of a non–tapering and tapering, fasciculated, single and geminate false branch- ing heterocytous cyanobacterium were collected from rocky shores in the Pacific Ocean and Gulf of Mexico. The populations were provisionally placed in Brasilonema based on morphology, but upon sequencing of both environmental and culture material it was discovered that the populations/cultures belonged to the Rivulariaceae, in a marine subclade of the family containing Kyrtuthrix huatulcensis. In culture, the taxon exhibited tapering in isopolar filaments, providing further evidence that it was a member of the rivulariacean clade. Based on molecular data for other cyanobacteria within the rivulariacean clade, …


Sweating The Small Stuff: Linking Plankton To Climate Change, Brian Kim '18 Makes Conncetions, Stephen Collins Sep 2016

Sweating The Small Stuff: Linking Plankton To Climate Change, Brian Kim '18 Makes Conncetions, Stephen Collins

Colby Magazine

It takes an expansive mind to connect microscopic marine copepods (certain crustacean plankton) unwittingly chomping on floating microplastics with a bigger picture: the planet’s carbon pump and global climate change. But that’s what Brian Kim ’18 decided to investigate during Jan Plan, working with Bigelow Lab Senior Research Scientist David Fields.


Host-Parasite Relationships Between The Copepod Naobranchia Lizae And Its Host (Striped Mullet, Mugil Cephalus): A Description Of Morphological Development, Sara R. Teemer Jul 2016

Host-Parasite Relationships Between The Copepod Naobranchia Lizae And Its Host (Striped Mullet, Mugil Cephalus): A Description Of Morphological Development, Sara R. Teemer

DePaul Discoveries

The parasitic copepod, Naobranchia lizae, is often found within the gill arches of the striped mullet, Mugil cephalus, in the Charleston Harbor Estuarine System of South Carolina. The parasite is relatively common, but little is known about its early stages of development. In order to describe the developmental stages and pattern of morphological development, 221 female N. lizae that were collected between February 2002 and May 2003 were used. Using variation in morphological characters (maxilla, trunk), these parasites were assigned to developmental stages (juvenile, subadult, adult). A small number of the males (three ‘dwarf’ males) were also found …


Ocean Acidification And Predator-Prey Relations: Correlating Disruption Of Predator Avoidance With Chemosensory Deficits, Alexandra Fw Sidun, William G. Wright May 2016

Ocean Acidification And Predator-Prey Relations: Correlating Disruption Of Predator Avoidance With Chemosensory Deficits, Alexandra Fw Sidun, William G. Wright

Student Scholar Symposium Abstracts and Posters

One of the most destructive effects of global climate change is the increased carbon sequestering and consequential acidification of our world’s oceans. The impacts of ocean acidification on marine organisms are still relatively unknown, especially effects on behavioral ecology. Avoiding predation has emerged from recent behavioral ecology literature as a critical feature in the life history of a wide array of animal species; experiments on marine fishes suggest acidic water compromises their predator-avoidance abilities. Recent assays in our lab suggest predator-induced behavior is reduced by weakly acidic water. These experiments do not address the potential factor of generalized malaise caused …


Isolation And Identification Of Bacterial Endosymbionts In The Brooding Brittle Star Amphipholis Squamata, Abbey Rose Tedford Jan 2016

Isolation And Identification Of Bacterial Endosymbionts In The Brooding Brittle Star Amphipholis Squamata, Abbey Rose Tedford

Honors Theses and Capstones

Symbiotic associations with subcuticular bacteria (SCB) have been identified and studied in numerous echinoderms, including the SCB of the brooding brittle star, Amphipholis squamata. These SCB, however, have not been studied using current next generation sequencing technologies. Previous studies on the SCB of A. squamata placed these bacteria in the genus Vibrio (γ-Proteobacteria), but subsequent studies suggested that the SCB are primarily composed of α-Proteobacteria. The present study examines the taxonomic composition of SCB associated with A. squamata from the Northwest Atlantic. DNA was extracted using a CTAB protocol and 16S rRNA sequences were amplified …


Physiological Performance Of Warm-Adapted Marine Ectotherms : Thermal Limits Of Mitochondrial Energy Transduction Efficiency., Eloy Martinez, Eric Hendricks, Michael Menze, Joseph Torres Aug 2015

Physiological Performance Of Warm-Adapted Marine Ectotherms : Thermal Limits Of Mitochondrial Energy Transduction Efficiency., Eloy Martinez, Eric Hendricks, Michael Menze, Joseph Torres

Faculty Scholarship

Thermal regimes in aquatic systems have profound implications for the physiology of ectotherms. In particular, the effect of elevated temperatures on mitochondrial energy transduction (i.e. energy from carbon substrates to ATP) in tropical and subtropical teleosts may have profound consequences on organismal performance and population viability. Upper and lower whole-organism critical temperatures for teleosts suggest that subtropical and tropical species are not susceptible to the warming trends associated with climate change, but sub-lethal effects on energy transduction efficiency and population dynamics remain unclear. The goal of the present study was to compare the thermal sensitivity of processes associated with mitochondrial …


Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen Aug 2013

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …


Edges And Overlaps In Northwest Atlantic Phylogeography, Safra Altman, John D. Robinson, James M. Pringle, James E. Byers, John P. Wares Apr 2013

Edges And Overlaps In Northwest Atlantic Phylogeography, Safra Altman, John D. Robinson, James M. Pringle, James E. Byers, John P. Wares

Publications and Research

As marine environments change, the greatest ecological shifts—including resource usage and species interactions—are likely to take place in or near regions of biogeographic and phylogeographic transition. However, our understanding of where these transitional regions exist depends on the defining criteria. Here we evaluate phylogeographic transitions using a bootstrapping procedure that allows us to focus on either the strongest genetic transitions between a pair of contiguous populations, versus evaluation of transitions inclusive of the entire overlap between two intraspecific genetic lineages. We compiled data for the Atlantic coast of the United States, and evaluate taxa with short- and long-dispersing larval phases …


Family Matters: An Analysis Of Genetic Relatedness Of Tetraclita Rubescens (The Pink Volcano Barnacle) Over Several Spatial Scales At Monterey And Bodega Bay, California, Kelly N. Chang Jan 2013

Family Matters: An Analysis Of Genetic Relatedness Of Tetraclita Rubescens (The Pink Volcano Barnacle) Over Several Spatial Scales At Monterey And Bodega Bay, California, Kelly N. Chang

Scripps Senior Theses

Inbreeding involves the mating of closely related individuals at a higher frequency than at random; this can decrease the average fitness of populations and individuals by reducing the presence of heterozygotes and augmenting the expression of deleterious genes. Since marine invertebrates exhibit widespread dispersal, their potential for inbreeding is often disregarded. The adult sessile state of barnacles creates the potential for inbreeding as a result of necessary copulation between neighboring individuals. Depending on the degree of mixing that occurs during dispersal, closely related individuals or siblings may settle in close proximity, generating the possibility of kin aggregation and consequent inbreeding. …


An Autonomous Vehicle Approach For Quantifying Bioluminescence In Ports And Harbors, Mark A. Moline, Paul Bissett, Shelley Blackwell, James Mueller, Jeff Sevadjian, Charles Trees, Ron Zaneveld Mar 2005

An Autonomous Vehicle Approach For Quantifying Bioluminescence In Ports And Harbors, Mark A. Moline, Paul Bissett, Shelley Blackwell, James Mueller, Jeff Sevadjian, Charles Trees, Ron Zaneveld

Biological Sciences

Bioluminescence emitted from marine organisms upon mechanical stimulation is an obvious military interest, as it provides a low-tech method of identifying surface and subsurface vehicles and swimmer tracks. Clearly, the development of a passive method of identifying hostile ships, submarines, and swimmers, as well as the development of strategies to reduce the risk of detection by hostile forces is relevant to Naval operations and homeland security. The measurement of bioluminescence in coastal waters has only recently received attention as the platforms and sensors were not scaled for the inherent small-scale nature of nearshore environments. In addition to marine forcing, many …