Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Assessing The Prevalence Of Multidrug Resistant Salmonella Enterica From Stream Sediment And Poultry Litter In The Shenandoah Valley Of Virginia, Noah Greenman
Masters Theses, 2010-2019
Multidrug resistant Salmonella enterica present in stream sediment and poultry litter represent a critical health concern. A small number of S. enterica serotypes are responsible for most lab-confirmed infections in the US each year. To assess the prevalence of these significant strains, we isolated 88 S. enterica from stream sediment and poultry litter. Sequence data for all isolates were generated using an Illumina® sequencing platform, with long-reads for some isolates from the Oxford Nanopore MinION™ used in a hybrid genome assembly approach. Isolates were typed according to their serotype and multi-locus sequence type using SeqSero/SISTR and Enterobase respectively. Antibiotic resistance …
The Potential For Replication And Transmission Of Antibiotic Resistance Plasmids In An E. Coli Population In Agriculturally Impacted Stream Sediment, Erika Gehr
Masters Theses, 2010-2019
The use of antibiotics in agriculture is thought to be a major cause of resistance in microorganisms found in the environment. Horizontal transfer of genetic information from transient to native and from native to transient bacterial populations may enhance the spread and recombination of resistance genes and might play a role in the formation of multi-resistant organisms in environmental reservoirs. Tetracycline resistance plasmids were compared using three isolation techniques -- traditional “endogenous” extraction from isolates, “exogenous” plasmid capture, and direct plasmid extraction from sediment --to determine the potential for plasmid born resistance in an E. coli population found in agriculturally-impacted …