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Full-Text Articles in Life Sciences
Exploring The Biofilm Of Candida Auris As It Relates To Persistence In Clinical Setting And Susceptibility To Antimicrobials, Bethany Butts
Exploring The Biofilm Of Candida Auris As It Relates To Persistence In Clinical Setting And Susceptibility To Antimicrobials, Bethany Butts
Master of Science in Integrative Biology Theses
The rapid rise of drug-resistant pathogens is a global health concern. Known pathogens are acquiring resistance to many commonly used drugs, while new drug-resistant pathogens are emerging. These resistant pathogens are responsible for many healthcare-associated infections (HAI) [1, 2]. Candida auris is among these emerging drug-resistant pathogens, first described in 2009. The CDC labels C. auris at the highest level of global public health threat [3,4]. Effective methods of decolonization and control of the organisms have yet to be established, as it is resistant to many agents used for microbial control. Recent research has found that C. auris has the …
A Shift To Human Body Temperature (37°C) Rapidly Reprograms Multiple Adaptive Responses In Escherichia Coli That Would Facilitate Niche Survival And Colonization, Anastasia Gant Kanegusuku, Isidora N. Stankovic, Pamela A. Cote-Hammarlof, Priscilla H. Yong, Christine A. White-Ziegler
A Shift To Human Body Temperature (37°C) Rapidly Reprograms Multiple Adaptive Responses In Escherichia Coli That Would Facilitate Niche Survival And Colonization, Anastasia Gant Kanegusuku, Isidora N. Stankovic, Pamela A. Cote-Hammarlof, Priscilla H. Yong, Christine A. White-Ziegler
Biological Sciences: Faculty Publications
One of the first environmental cues sensed by a microbe as it enters a human host is an upshift in temperature to 37°C. In this dynamic time point analysis, we demonstrate that this environmental transition rapidly signals a multitude of gene expression changes in Escherichia coli. Bacteria grown at 23°C under aerobic conditions were shifted to 37°C, and mRNA expression was measured at time points after the shift to 37°C (t = 0.5, 1, and 4 h). The first hour is characterized by a transient shift to anaerobic respiration strategies and stress responses, particularly acid resistance, indicating that temperature serves …
Periodically Disturbing The Spatial Structure Of Biofilms Can Affect The Production Of An Essential Virulence Factor In Pseudomonas Aeruginosa, Rebecca J. Quinn, Ivana Barraza, Laura García-Diéguez, Camryn Pajon, Lauren E. Krausfeldt, Kerollos Ibrahim, Laura A. Enzinna, Morgan E. Thorn, Omar Tonsi Eldakar, Travis J. A. Craddock, Robert P. Smith
Periodically Disturbing The Spatial Structure Of Biofilms Can Affect The Production Of An Essential Virulence Factor In Pseudomonas Aeruginosa, Rebecca J. Quinn, Ivana Barraza, Laura García-Diéguez, Camryn Pajon, Lauren E. Krausfeldt, Kerollos Ibrahim, Laura A. Enzinna, Morgan E. Thorn, Omar Tonsi Eldakar, Travis J. A. Craddock, Robert P. Smith
Biology Faculty Articles
Understanding the environmental factors that affect the production of virulence factors has major implications in evolution and medicine. While spatial structure is important in virulence factor production, observations of this relationship have occurred in undisturbed or continuously disturbed environments. However, natural environments are subject to periodic fluctuations, including changes in physical forces, which could alter the spatial structure of bacterial populations and impact virulence factor production. Using Pseudomonas aeruginosa PA14, we periodically applied a physical force to biofilms and examined production of pyoverdine. Intermediate frequencies of disturbance reduced the amount of pyoverdine produced compared to undisturbed or frequently disturbed conditions. …
Isolating Oral Bacterial Species From A Single Donor Through A Multi-Step Detection Method, Khalid Al-Lakhen
Isolating Oral Bacterial Species From A Single Donor Through A Multi-Step Detection Method, Khalid Al-Lakhen
Legacy Theses & Dissertations (2009 - 2024)
The human oral microbiome is one of the most complex microbiome communities in the human body and its role in maintaining health is not fully understood. Previous research shows that the transition from health to disease is accompanied by changes in community abundance and structure. To fully understand the symbiotic relationship formed between bacterial species that leads to the formation of a functional healthy biofilm structure, we must study the various structural relationships and subsequent molecular interactions among individual species within the biofilm. In this thesis, I have isolated a pure bacteria species from a single donor's dental plaque and …