Life Sciences Commons^™

Exploring The Effects Of Hypoxia On Sulfate Reducing Anaerobes, Annaliese K. Jones

Senior Honors Projects

Exploring the Effects of Hypoxia on Sulfate Reducing Anaerobes

Annaliese K. Jones

Sponsor: Bethany Jenkins, Cell and Molecular Biology

As a student about to graduate with a degree in Biological Sciences, I find myself faced with the need to find my own independent research interests and scientific voice. As a result of my interests in the fields of both microbiology and ecology, I am drawn to questions surrounding the role and behavior of microorganisms in the environment. With climate change being an issue capturing the attention of a large portion of the scientific community, I have chosen to focus my …

Tissue-Specific Thyroid Hormone Regulation Of Gene Transcripts Encoding Iodothyronine Deiodinases And Thyroid Hormone Receptors In Striped Parrotfish (Scarus Iseri), Kaitlin M. Johnson, Sean C. Lema

Biological Sciences

In fish as in other vertebrates, the diverse functions of thyroid hormones are mediated at the peripheral tissue level through iodothyronine deiodinase (dio) enzymes and thyroid hormone receptor (tr) proteins. In this study, we examined thyroid hormone regulation of mRNAs encoding the three deiodinases dio1, dio2 and dio3 – as well as three thyroid hormone receptors trαA, trαB and trβ – in initial phase striped parrotfish (Scarus iseri). Parrotfish were treated with dissolved phase T₃ (20 nM) or methimazole (3 mM) for 3 days. Treatment with exogenous T₃ elevated circulating T₃, while the …

Growth Phase-Dependent Gene Regulation In Vivo In Sulfolobus Solfataricus, Melissa Deyoung, Michelle Thayer, John Van Der Oost, Kenneth M. Stedman

Biology Faculty Publications and Presentations

Ribosomal genes are strongly regulated dependent on growth phase in all organisms, but this regulation is poorly understood in Archaea. Moreover, very little is known about growth phase-dependent gene regulation in Archaea. SSV1- based lacS reporter gene constructs containing the Sulfolobus 16S/23S rRNA gene core promoter, the TF55a core promoter, or the native lacS promoter were tested in Sulfolobus solfataricus cells lacking the lacS gene. The 42-bp 16S/23S rRNA gene and 39-bp TF55a core promoters are sufficient for gene expression in S. solfataricus. However, only gene expression driven by the 16S/23S rRNA gene core promoter is dependent on …

Analysis Of Common And Specific Mechanisms Of Liver Function Affected By Nitrotoluene Compounds, Youping Deng, Sharon A. Meyer, Xin Guan, Barbara Lynn Escalon, Junmei Ai, Mitchell S. Wilbanks, Ruth Welti, Natàlia Garcia-Reyero, Edward J. Perkins

Faculty Publications

Background: Nitrotoluenes are widely used chemical manufacturing and munitions applications. This group of chemicals has been shown to cause a range of effects from anemia and hypercholesterolemia to testicular atrophy. We have examined the molecular and functional effects of five different, but structurally related, nitrotoluenes on using an integrative systems biology approach to gain insight into common and disparate mechanisms underlying effects caused by these chemicals.

Methodology/Principal Findings: Sprague-Dawley female rats were exposed via gavage to one of five concentrations of one of five nitrotoluenes [2,4,6-trinitrotoluene (TNT), 2-amino-4,6-dinitrotoluene (2ADNT) 4-amino-2,6-dinitrotoulene (4ADNT), 2,4-dinitrotoluene (2,4DNT) and 2,6-dinitrotoluene (2,6DNT)] with necropsy …

Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel

Turkish Journal of Biology

Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …