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Full-Text Articles in Life Sciences
The Electron Microscope Detection And X-Ray Quantitation Of Cations In Bacterial Cells, D. C. Sigee, M. H. El-Masry, R. H. Al-Rabaee
The Electron Microscope Detection And X-Ray Quantitation Of Cations In Bacterial Cells, D. C. Sigee, M. H. El-Masry, R. H. Al-Rabaee
Scanning Electron Microscopy
Electron microscope autoradiography and X-ray microanalysis have been used for the detection and quantitation of cations in the bacterium Pseudomonas tabaci. These techniques differ in the information they provide (relating to either cation uptake or in situ levels), their applicability to different cations, their sensitivity and their spatial resolution. With uptake of 63Ni2+, high resolution autoradiography (involving gold latensification and physical development) demonstrated a high degree of cation localisation to the central nucleoid area (glutaraldehyde-fixed cells) and within this to the constituent chromatin (acetic-alcohol preparations).
X-ray microanalysis of whole bacterial cells revealed the presence of substantial levels …
Two Opposing Theories Of The Cell: Experimental Testing By Cryomethods And Electron Microscopy, L. Edelmann
Two Opposing Theories Of The Cell: Experimental Testing By Cryomethods And Electron Microscopy, L. Edelmann
Scanning Electron Microscopy
A main controversial issue in cell biology concerns the molecular mechanism responsible for K+ accumulation in living cells and Na+ exclusion from them. The alternative theoretical descriptions of these phenomena are based on different assumptions about the physical state of cellular Na+, K+ and H2O. In this article it is shown with striated muscles how cryomethods and microanlytical electron microscopy may be used to test the opposing theories. It is concluded that these methods may yield more realistic informations about the physical state of cellular K+ and Na+ than measurements with …