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An Investigation Of Mitochondrial Bioenergetics And The Turnover Of Succinated Proteins In The Adipocyte During Diabetes, Ross Tanis
Theses and Dissertations
We previously identified the chemical modification S-(2-succino)cysteine (2SC), which is formed when the Krebs cycle metabolite fumarate reacts with protein cysteine residues, also termed protein succination. Protein succination is increased in the adipose tissue of ob/ob and db/db mice in vivo and in 3T3-L1 adipocytes grown in high glucose in vitro. The increase in 2SC in the 3T3-L1 adipocyte occurs as a direct result of glucotoxicity and increased mitochondrial stress. We have shown that uncoupling agents, which lower mitochondrial stress, prevent the increase in succinated proteins.
In this study we examined the relationship between increased succination and mitochondrial bioenergetics in …