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Articles 1 - 30 of 47
Full-Text Articles in Life Sciences
The Effect Of Wee1 On Mitosis In The Spb Mutant, David Leroy
The Effect Of Wee1 On Mitosis In The Spb Mutant, David Leroy
Honors Theses
The speedbump mutant has been identified as an early arrest mutant, becoming apparent as soon as 80% epiboly (8.5 hours of development). This mutant has a mutation in wee1, which is a protein regulating entry into mitosis ensuring the completion of DNA replication before mitosis. The lack of this protein in speedbump allows for early entry into mitosis resulting in damage to the chromosome. Here, we investigate two questions: if mitosis in the speedbump mutant phenotype is more frequent than wild-type phenotype, and, if so, is there a phase in which the mutant phenotype favors. We used phosphorylated histone H3 …
Weight Management Delivered Through An App, May Help Resolve Body Image Dissatisfaction In Women Enrolled In Wic, Alexis Ariel O’Connell
Weight Management Delivered Through An App, May Help Resolve Body Image Dissatisfaction In Women Enrolled In Wic, Alexis Ariel O’Connell
Honors Theses
No abstract provided.
Expression Of Glycine-Rich Proteins Found In Salivary Glands Of The Lone Star Tick (Amblyomma Americanum) Using A Mammalian Cell Line, Annabelle Clark
Expression Of Glycine-Rich Proteins Found In Salivary Glands Of The Lone Star Tick (Amblyomma Americanum) Using A Mammalian Cell Line, Annabelle Clark
Honors Theses
Ticks play an important ecological role as well as a growing role in human health and veterinary care. Ticks are hosts to a plethora of microbial pathogens that can be transferred during feeding to cause tick-borne diseases in humans and many animals. Ticks may in large part owe the success of the transfer of these pathogens between hosts to their complex saliva. The saliva secreted upon a tick’s attachment to a host serves the following, among other, functions: anti-hemostasis of the blood pool, preventing an inflammatory response at the bite site, and serving as a natural anti-microbial substance. An important …
The Phenology And Compatibility Of Hazelnut (Corylus Avellana) Cultivars In Tennessee, Ben Nigel Heard
The Phenology And Compatibility Of Hazelnut (Corylus Avellana) Cultivars In Tennessee, Ben Nigel Heard
Honors Theses
The European hazelnut (Corylus avellana L.), is an important temperate zone nut tree species for which there is an expanding demand worldwide. Historically, the Eastern filbert blight disease (EFB), caused by the ascomycete fungus Anisogramma anomala, has prevented commercial hazelnut growing in Tennessee. As part of a UTC hazelnut cultivar trial, EFB resistant hazelnut cultivars and numbered selections from Oregon State University and Burnt Ridge Nursery were planted in 2003 at Smith Farm in Ooltewah, TN. Hazelnut trees are wind-pollinated, dichogamous, and self-incompatible, which means they are not self-fertile, their male and female blossoms may open at different times, and …
Association Of Glucocorticoid Receptor Polymorphisms With Metabolic Characteristics And Bariatric Surgery In Bariatric Patients, Sebastien Gingras
Association Of Glucocorticoid Receptor Polymorphisms With Metabolic Characteristics And Bariatric Surgery In Bariatric Patients, Sebastien Gingras
Honors Theses
Cortisol acts on target tissues through the glucocorticoid receptor (GR). When in high concentration in the blood, it causes an obesity phenotype with hyperglycemia, hyperglyceridemia, hypertension and weight gain, known as Cushings’ Syndrome or Disease (CS/D). Hypersensitivity-causing single-nucleotide polymorphisms (SNPs) of the GR have been shown to lead to a similar phenotype. Because of the symptomatic resemblance between CS/D and Metabolic Syndrome (MS), we and others hypothesized that the MS may be a Cushingoid-like state with absence of hypercortisolemia, but with GR hypersensitivity. Additionally, the link between GR SNPs and the success of bariatric surgery as measured by the percent …
Learning And Memory In A Drosophila Melanogaster Model Of Alzheimer's Disease, Eric Robles
Learning And Memory In A Drosophila Melanogaster Model Of Alzheimer's Disease, Eric Robles
Honors Theses
Human Alzheimer's disease (AD) is the most prevalent and lethal neurodegenerative disease; it involves the accumulation of neurofibrillary tangles, loss of synapses and neurons in specific areas of the brain, and the presence of extracellular amyloid plaques, particularly Amyloid beta-42 (AP-42). In this study, two Drosophila transgenic fly lines carrying either elav-GAL4 driver or UAS-AP-42 transgene, were crossed to generate AD flies that expressed low levels of human Ap-42. Male AD flies (experimental) and elav-GAL4 flies (as parental control without AP-42) were tested for learning and short-term memory using the courtship suppression assay (Siegal and Hall, 1979). The courtship suppression …
Towards A Better Understanding Of Antiviral Rnai In Caenorhabditis Elegans: Function Characterization Of Loss Of Antiviral Rnai Alleles 1026a And 1026d, Drake Leonard
Honors Theses
No abstract provided.
An Analysis Of Environmentally-Persistent Free Radical Formation On Surrogate Soil Systems Containing Iron(Iii) And Copper(Ii) And The Potential Remediation Effects Of Laccases, Andrew Paul Mullet
An Analysis Of Environmentally-Persistent Free Radical Formation On Surrogate Soil Systems Containing Iron(Iii) And Copper(Ii) And The Potential Remediation Effects Of Laccases, Andrew Paul Mullet
Honors Theses
No abstract provided.
Using Intrinsic Properties Of Polyaniline To Sense Expression Of The Microrna Let-7, Jared N. Gloria
Using Intrinsic Properties Of Polyaniline To Sense Expression Of The Microrna Let-7, Jared N. Gloria
Honors Theses
MicroRNAs are approximately 22-nucleotide long RNA molecules that function through decay and translational repression of messenger RNA. The microRNA let-7 is found to play a role in maintaining the fate of differentiated cells in humans. Thus, expression level of this microRNA is a reliable biomarker of tumor cell phenotype. However, there are significant limitations in the current profiling techniques of microRNA. The current methods like northern blotting, microarrays, RT-PCR, or using locked nucleic acid (LNA) for in-situ hybridization are either laborious, semi-quantitative, or expensive. In this research we try to address this issue by developing a fast, specific, and inexpensive …
The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero
The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero
Honors Theses
Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco
Honors Theses
A Klebsiella pneumoniae bacteriophage was isolated from waste water and purified over multiple rounds of plaque removals and serial titrations. This bacteriophage was imaged in a transmission electron microscope and then tested with selected antibiotics for effectiveness in reducing optical density of K. pneumoniae colonies. The phage was shown to have an increased effectiveness in reducing the optical density when used together with an antibiotic that the bacteria was susceptible to.
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson
Honors Theses
Consumption of a prominent synthetic cathinone known as mephedrone (4-methylmethcathinone) has become a popular alternative to club drugs such as ecstasy, cocaine, and methamphetamine within the past decade. The pharmacological mechanisms that contribute to its subjective effects have yet to be fully characterized and are thus warranted for investigation. The present study employed drug discrimination methods to train eight male Sprague-Dawley rats to discriminate injections of 3.0 mg/kg mephedrone from saline. Various doses of mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), d-amphetamine (AMPH), cocaine, (+)-methamphetamine (METH), and 3,4-methylenedioxy-methamphetamine (MDMA) were investigated for their ability to substitute for the stimulus cues of the training …
Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker
Chemosensory Signals From Gravid Females Elicit Behavioral Response From Dominant Male Astatotilapia Burtoni, Cristopher T. Mcvicker
Honors Theses
No abstract provided.
Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom
Analysis Of Western Lowland Gorilla (Gorilla Gorilla Gorilla) Specific Alu Repeats, Thomas Beckstrom
Honors Theses
No abstract provided.
The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster
The Effects Of Mitochondrial Dna On The Fitness Of The Marine Crustacean Tigripus Californicus, Ellen Foster
Honors Theses
No abstract provided.
One Signal, Two Behaviors: Odor Discrimination In Unmated Versus Mated Female Green Bottle Flies, Lucilia Sericata, George Iannantuono
One Signal, Two Behaviors: Odor Discrimination In Unmated Versus Mated Female Green Bottle Flies, Lucilia Sericata, George Iannantuono
Honors Theses
The green bottle fly, Lucilia sericata, is of critical importance in the field of forensic entomology since it is one of the first insects to arrive at a freshly deceased carcass. These flies use a highly tuned and selective olfactory system to identify and locate the carcass; response to the decomposition odor usually occurs within minutes. Male flies use the carcass for a small protein meal and for locating females while females use the carcass for feeding (unmated) and egg-laying (mated). This divergence in behavior to a common odor cue is the subject of this thesis proposal: do unmated …
Distribution Of Shell Formation Proteins In Oyster Hemolymph, Hemocytes, And Mantle Tissue, Donald Joseph Kleppel
Distribution Of Shell Formation Proteins In Oyster Hemolymph, Hemocytes, And Mantle Tissue, Donald Joseph Kleppel
Honors Theses
The occurrence and composition of L,3-4-dihydroxyphenylalanine-containing proteins (L-DOPA proteins) that participate in oyster shell formation has not been fully determined. It is known that the oyster mantle tissue is primarily responsible for shell formation and recent research has demonstrated the involvement of the hemolymph (blood) and hemocytes (blood cells). L-DOPA proteins are known to aid in the cross linking of shell formation proteins, in turn creating the insoluble organic matrix formed to produce the organic component of the shell. Using the biomarker amino acid L-DOPA, this research focuses on determining the localization of these shell formation proteins in hemocytes, hemolymph, …
Finding A Correlation Between Zooplankton Abundance And The Aggregation Of Abudefduf Saxatilis (Sergeant Major Damselfish) Beneath Boats, William Duritsch
Finding A Correlation Between Zooplankton Abundance And The Aggregation Of Abudefduf Saxatilis (Sergeant Major Damselfish) Beneath Boats, William Duritsch
Honors Theses
Abudefduf saxatilis, or sergeant major damselfish, are a common reef fish in the Caribbean and western Atlantic that form large feeding aggregations. Abudefduf saxatilis are primarily planktivorous, with zooplankton making up over 50% of their diet. Zooplankton are known to have diel movements to avoid predation, which have been shown to be triggered by the presence of ultra violet radiation. Beneath boats along the coast of Bonaire, aggregations of A. saxatilis have been observed, but why they prefer these areas over the open water column had not previously been examined. The abundance of zooplankton was estimated beneath boats as …
The Effects Of Low Dam Removal And Kayak Run Installation On The Biodiversity Of Fish And Macroinvertebrates In The Great Miami River In Downtown Dayton, Ohio, Sarah Anne Stalder
The Effects Of Low Dam Removal And Kayak Run Installation On The Biodiversity Of Fish And Macroinvertebrates In The Great Miami River In Downtown Dayton, Ohio, Sarah Anne Stalder
Honors Theses
Five Rivers Metroparks and the Miami Conservancy District have made plans to remove the upper portion of the Monument Avenue low-head dam in downtown Dayton due to the hazard it poses for recreation on the river and its negative impact on water quality and biodiversity. The plans also include modifying the dam with “kayak drops” and the construction of a second kayak drop slightly upstream of the location of the current low dam to enhance recreational opportunities on the river. Typically, low dam removal alter the flow and depth of a river, especially above the dam in the impounded area …
Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer
Evolution Of Alu Element Subfamilies In Papio Baboons, Jon Hunter Strohmeyer
Honors Theses
No abstract provided.
15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler
15-Lipoxygenase-2 Expression And Activity In Hek 293 Cells, Kirstie Rae Schieffler
Honors Theses
No abstract provided.
Characterization Of Ammonium Salt Toxicity In Cabbage Family Plants, Madison Boudreaux
Characterization Of Ammonium Salt Toxicity In Cabbage Family Plants, Madison Boudreaux
Honors Theses
No abstract provided.
Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons
Development Of Mouse Melanoma Model To Evaluate Oncolytic Viral Therapy Using Osv, Kelli A. Clemons
Honors Theses
No abstract provided.
A Compartmental Model Of Animal Behavior, Saharsh Dass
A Compartmental Model Of Animal Behavior, Saharsh Dass
Honors Theses
Animal behavior is integral to fitness and arises from complex interactions between internal and external factors. An understanding of how external environmental factors drive animal behavior is important for understanding the way organisms adapt to environmental perturbations such as climate change. Glaucous-winged gulls (Larus glaucescens) at Protection Island, Strait of Juan de Fuca, Washington display a variety of behaviors on the colony during the breeding season. The most common gull behaviors are sleeping, preening, and resting. I used a system of four differential equations to predict numbers of sleeping, preening, and resting gulls on the colony as a function of …
Determining The Concentration Of Nitric Oxide In Solvent, Emily-Jean E. Bankes
Determining The Concentration Of Nitric Oxide In Solvent, Emily-Jean E. Bankes
Honors Theses
The body creates nitric oxide (NO) for signaling. One way that researchers study NO signaling is through the use of model complexes, or molecules that have the same metal ion and a similar molecular shape as biological molecules and are easy to synthesize. Currently researchers have limited ways to deliver precisely-known small quantities of NO to an experiment where they are trying to investigate NO-binding to a transition metal complex. A peak shift in the absorption spectrum at ~530 nm makes it possible for UV-Vis spectroscopy to observe NO binding to cobalt tetraphenylporphyrin (CoTPP). This makes it possible to measure …
The Effects Of Omega-3 Fatty Acids On Intracellular Inositol Levels In Saccharomyces Cerevisiae, Jee Yeon (Joanne) Lee
The Effects Of Omega-3 Fatty Acids On Intracellular Inositol Levels In Saccharomyces Cerevisiae, Jee Yeon (Joanne) Lee
Honors Theses
Bipolar disorder is a severe and chronic debilitating mental disorder characterized by extreme mood swings between mania and depression. The current medications for bipolar disorder, lithium and valproate, have been associated with numerous negative side effects. Although the therapeutic mechanism by which lithium and valproate (VPA) exert their effect is unknown, a leading hypothesis implicates inositol depletion as a mechanism of action. On the other hand, omega-3-fatty acids have been shown to relieve symptoms of bipolar disorder. In this study, we compare the effects of VPA to the effects of decosahexaenoic acid (DHA) on Saccharomyces cerevisiae growth and intracellular inositol …
The Effect Of Degrading The Transcription Factor Nf-Kb Subunit Proteins On Nf-Kb' S Oncological Activity, Seongmin Lee
The Effect Of Degrading The Transcription Factor Nf-Kb Subunit Proteins On Nf-Kb' S Oncological Activity, Seongmin Lee
Honors Theses
Nuclear factor-kappa B (NF-κB) is a transcription factor that becomes functional when any two of its five component proteins (p50, p52, p65, c-Rel, and RelB) join together. NF-κB plays an important role in bringing out cell proliferation, or cell growth. When NF-κB malfunctions and becomes hyperactive, excessive NF-κB activity promotes abnormally high cell growth, which is a symptom of cancer. Because of its tie to cancer, NF-κB is commonly subjected to modification to curb cancer growth. In this project, each component protein of NF- κB was degraded via a method called RNAi to see if it would have any negative …
Injury-Induced Neuronal Turnover With Zinc Sulfate Affects Cilitated Olfactory Sensory Neurons More Than Microvillous Olfactory Sensory Neurons In The Adult Zebrafish, James Hentig
Honors Theses
Zinc sulfate is a known olfactory toxicant, although its effects on the olfactory epithelium of zebrafish have not been examined. Previous observations utilizing Triton X-100 found selective retention of some olfactory sensory neurons and loss of others. Based on those findings, we hypothesized that the retention of the ability to sense amino acids, detected by microvillous olfactory sensory neurons, and loss of the ability to perceive bile salts, detected by ciliated olfactory sensory neurons, is a universal response to chemical ablation. Fish were treated with zinc sulfate and survived for 2, 5, 10 and 14 days post treatment and processed …
Hormonal And Social Factors Influencing Alternative Mating Tactic Decisions In Male Green Treefrogs, Hyla Cinerea, Brooks Pratt
Hormonal And Social Factors Influencing Alternative Mating Tactic Decisions In Male Green Treefrogs, Hyla Cinerea, Brooks Pratt
Honors Theses
In many anuran species (frogs and toads), males alternate between calling and a non-calling satellite mating tactic. Satellite males characteristically associate with calling males and attempt to intercept females that are attracted to the vocalizations of the calling host male. The prevailing hypothesis is that the decision to adopt a calling or satellite mating tactic is based on relative attractiveness — males are predicted to adopt a satellite mating tactic when the vocalizations of nearby males are more attractive than their own. In support of this hypothesis, previous studies have shown that satellite males can be induced to call by …
Optimizing Rna Extraction From Fungal-Algal Tissues For Transcriptomic Gene Expression Analysis, Michael J. Hohl
Optimizing Rna Extraction From Fungal-Algal Tissues For Transcriptomic Gene Expression Analysis, Michael J. Hohl
Honors Theses
Extracting high-quality RNA is critical for downstream applications such a qRT-PCR and RNA-Seq based transcriptomics. Single-stranded RNA is readily susceptible to degradation by environmental RNases, necessitating rapid and sterile homogenization techniques; freezing at -80°C, lysis buffer addition, and short bursts of beadbeating have been shown to increase yields and quality of extracted RNA. Utilizing fungal-algal tissues from the model symbiosis between the filamentous fungi Aspergillus nidulans and the green alga Chlamydomonas reinhardtii, three different commercial homogenizers and six RNA extractions kits were assessed and an optimized extraction protocol for total RNA from fungal-algal tissues was determined. Using the protocol designed …