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Lcp1 Mutant Zebrafish: A Look At Neutrophils, Cancer, And Gene Compensation, Taylor Mitchell
Lcp1 Mutant Zebrafish: A Look At Neutrophils, Cancer, And Gene Compensation, Taylor Mitchell
College of Science and Health Theses and Dissertations
Lymphocyte cytosolic protein 1 (lcp1 or L-plastin) is a small actin-bundling protein that is typically only expressed in motile leukocytes, such as neutrophils and macrophages. However, it is also overexpressed in cancer cells, which may be related to tumor metastasis. Using CRISPR/Cas9 gene editing, our lab has created zebrafish that are genetic knockouts for lcp1 in order to better understand the relationship between L-plastin and cell motility.
Previous studies on L-plastin knockout mice have shown that the mutants have a decreased immune response, and therefore I predicted that our zebrafish mutants might have impaired development or distribution of immune cells. …