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Full-Text Articles in Life Sciences
Designing New Biomaterials: Modifying A Spider Silk Gene For Efficient Bacterial Expression For Industrial Production, Tanner J. Hancock, Nathan A. Payne, Travis Hotchkiss, Fernando A. Agarraberes, Randolph V. Lewis
Designing New Biomaterials: Modifying A Spider Silk Gene For Efficient Bacterial Expression For Industrial Production, Tanner J. Hancock, Nathan A. Payne, Travis Hotchkiss, Fernando A. Agarraberes, Randolph V. Lewis
Biology Posters
Spider silks have remarkable physical properties due to a combination of strength and elasticity. In addition, spider silks are biocompatible and biodegradable. Our laboratory has shown that the strength of products, such as fibers, produced with other silk proteins correlates with the size of the silk protein. The aciniform silk protein (AcSp1), has been shown to produce the thinnest and strongest fibers of all the natural spider silks. Aciniform silk is composed of a nonrepetitive amino-terminal region, 14 repeats of approximately 200 amino acids each, and a nonrepetitive carboxy-terminal region. We have been able to produce different variants of this …
Optimization Of Recombinant Spider Silk Protein Purification From Goat Milk, Brandon Yazzie, Michael Valentine, Tanner Hancey, Kaedon Buchmiller, Justin Jones, Randy Lewis
Optimization Of Recombinant Spider Silk Protein Purification From Goat Milk, Brandon Yazzie, Michael Valentine, Tanner Hancey, Kaedon Buchmiller, Justin Jones, Randy Lewis
Biology Posters
Over millions of years, spiders have evolved to produce a biocompatible material known as spider silk. Nephila clavipes, more commonly known as the Golden Orb Weaving Spider, is one of the most studied. Orb weaving spiders produce 7 different types of silk with unique functions and properties.
Molecular Cloning, Josh Worley
Molecular Cloning, Josh Worley
Biology Posters
Molecular cloning is a process that manipulates the spider silk sequence to select for a proper sequence size and specific vector into which it can be expressed. This process is achieved using techniques such as digests, transformations, purifications, and ligations. This semester, our work has centered around the mPRI(alfalfa), pOET2(insect), and pmk(ecoli) vectors, into which we have, or will, insert 3 time, 6 time, and 9 time sequences of the spider silk amino acid chain. With each repetition of the silk sequence, its properties improve and become more like natural silk. Vector specification allows these sequences to be produced in …