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Summer 2017 Internship At The Forage And Range Research Lab Usda, Braydon Marchant
Summer 2017 Internship At The Forage And Range Research Lab Usda, Braydon Marchant
Biology Posters
I had the privilege to work with several researchers and lab technicians at the Forage and Range Research Lab, which is part of the United States Department of Agriculture. The work I did primarily dealt with the molecular genetics of grasses, particularly those of the Poa genus.
Molecular Cloning, Josh Worley
Molecular Cloning, Josh Worley
Biology Posters
Molecular cloning is a process that manipulates the spider silk sequence to select for a proper sequence size and specific vector into which it can be expressed. This process is achieved using techniques such as digests, transformations, purifications, and ligations. This semester, our work has centered around the mPRI(alfalfa), pOET2(insect), and pmk(ecoli) vectors, into which we have, or will, insert 3 time, 6 time, and 9 time sequences of the spider silk amino acid chain. With each repetition of the silk sequence, its properties improve and become more like natural silk. Vector specification allows these sequences to be produced in …