Life Sciences Commons^™

Determining The Functional Roles Of Short Open Reading Frames In Mycobacterium Smegmatis, Lauren Fox

Biological Sciences

The study examines the roles of a subset of short open reading frames (sORFs) in M. smegmatis using targeted mutagenesis and the subsequent examination of phenotypes associated with sORF mutation or overexpression. sORFs are defined as stretches of nucleic acid encoding a protein with a maximum of 50 amino acids. Genome annotation pipelines overlook sORFs encoding small proteins (sproteins) and, thus, sproteins in bacteria have gone unnoticed, even those that are expressed at levels similar to larger, well-described proteins. As a result of recent advances in ribosome profiling and mass spectrometry, hundreds of previously unannotated sORFs have been identified, increasing …