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Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae, John Kelly Dougherty
Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae, John Kelly Dougherty
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Two closely related Caenorhabditis species, C. briggsae and C. nigoni are cross fertile and produce viable adult progeny. From C. nigoni mothers, F1 adult females are viable and fertile, F1 males are viable but sterile. In crosses that utilize C. nigoni males and C. briggsae hermaphrodites produce viable adult F1 females but F1 males arrest during embryogenesis. A mutation in the Cbr-him-8 gene is a recessive maternal-effect suppressor of male-specific lethality. Hybrid crosses with cbr-him-8 mutant mothers produce viable adult male progeny. The HIM-8 protein in C. elegans is required for the pairing of X-chromosomes during meiosis. This function is …
Mapping Hybrid Lethal Genes On The X Chromosome Of C. Briggsae, Blaine E. Bittorf
Mapping Hybrid Lethal Genes On The X Chromosome Of C. Briggsae, Blaine E. Bittorf
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In the cross of C. nigoni males to C. briggsae hermaphrodites, all F1 males arrest during embryogenesis. However in the reciprocal cross there are some viable F1 male progeny. This unidirectional male-specific lethality in the F1 hybrids has been attributed to a hybrid lethal gene in a 500 Kb region of the X chromosome of C. briggsae. Cbr-him-8 is a recessive maternal suppressor of the male-specific lethal phenotype, due to the requirement of the him-8 protein for proper X chromosome pairing. Without proper pairing of any one of the chromosomes in the Caenorhabditis genome, genes present on the unpaired chromosome …
Assortative Fertilization In The Elegans-Group Of Caenorhabditis, Sara Rose Seibert
Assortative Fertilization In The Elegans-Group Of Caenorhabditis, Sara Rose Seibert
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Assortative fertilization refers to the species-specific interactions between sperm and oocytes that affect the success of fertilization. One type of interaction is chemotaxis of sperm to oocytes. In Caenorhabditis elegans, amoeboid sperm must crawl along the uterine lining towards the spermathecae in response to oocyte-derived prostaglandin signals for fertilization. This chemotactic signaling system likely operates in other species of the Elegans-Group of Caenorhabditis as sperm of C. briggsae and C. remanei do localize to the C. elegans spermathecae. In this project the impacts of species-specific chemotaxis on fertilization and female fecundity were assessed. To accomplish this, the localization of …