Life Sciences Commons^™

Partial Purification & Kinetic Properties Of Nicotinamide Adenine Dinucleotide Phosphate-Dependent Alcohol Dehydrogenase Of Phycomyces Blakesleeanus, Thomas Hartz

Masters Theses & Specialist Projects

NADP⁺-dependent alcohol dehydrogenase (alcohol:NADP⁺:oxidoreductase, EC 1.1.1.2.) was partially purified from a cell-free extract of Phycomyces blakesleeanus (+ mating type, Vanderbilt strain). The fungus was grown in shake cultures containing glucose-asparagine broth, and crude cull-free enzyme extracts were prepared. The crude enzyme extracts were partially purified by a combination of ammonium sulfate precipitations and heat precipitation. Purification resulted in a 40;5 recovery of the enzyme with an increase in specific activity from 0.64 units per milligram of protein to 0.25 units per milligram. The partially-purified enzyme extracts were used to determine the kinetic properties and substrate specificity …

The Life History Of The Teardrop Darter, Etheostoma Barbouri Kuehne And Small, In Kentucky, Rory Flynn

Masters Theses & Specialist Projects

The life history of the teardrop darter, Etheoctoma (Catonotus) barbouri Kuehne and Small, was intensively studied from September, 1972, through August 1973. A total of 299 teardrop darters was seined from two isolated populations in Alexander and Little Muddy Creeks, Kentucky. These darters are known only to occur in second, third or fourth order tributaries of the Green River in Kentucky and Tennessee, and normally seek protection afforded by rocky or vegetated edges of such streams.

Gonosomatic ratios indicated that teardrop darters spawned from late March through late May in waters 12.5 - 15.5 C. Young females developed primary ova …

Comparative Bioenergetics Of Insects From Young & Old Plant Ecosystems, Joyce Wright

Masters Theses & Specialist Projects

Caloric values of insects and arachnids from two woods and two meadow ecosystems were compared. No significant difference was found between the orders Diptera, Homoptera, Orthoptera, Hemiptera, Hymenoptera, or Coleoptera. Caloric values of the class Arachnida were significantly higher in the woods ecosystem at the .05 level of significance. The order Lepidoptera differed at the .01 level of significance and was also higher in the woods samples. For all orders combined the caloric values of the woods samples were higher than the meadow samples at the .001 level of significance.

No consistent pattern was found in seasonal variation in caloric …

Anatomical & Phenological Variation Of Liquidambar Styraciflua L. Under Controlled Environmental Conditions, William Randel

Masters Theses & Specialist Projects

A large degree of variation in fiber tracheid length, wood specific gravity, and time of apical bud formation existed within two populations of Liquidambar styraciflua L. from south central Kentucky. The variation exhibited by these populations may be attributed to the self-sterility of Liquidambar and the variable environment of south central Kentucky.

Variation of fiber tracheid length and wood specific gravity within the two populations was significant. Fiber tracheid length was dependent on temperature and photoperiod while wood specific gravity was primarily dependent on temperature. A significant level of variation was also evident between the two populations regarding tracheid length. …

The Metabolism Of Tartaric Acid By Aspergillus Ficuum, Larry Daniel

Masters Theses & Specialist Projects

Soil samples were screened for organisms which decompose tartaric acid as an energy source. Several microorganisms were isolated which were capable of metabolizing tartaric acid. The organism which grew best was identified as Aspergillus ficuum and used in this study. This organism, when grown in tartrate medium, resulted in a decrease in optical rotation and density of the culture medium. The pH of the culture filtrate increased with a concurrent increase in mycelial weight. Analysis of culture filtrates by gas chromatography indicate that the first intermediate compound detected in tartrate decomposition by A. ficuum is glycolic acid. The terminal metabolite …

Structure & Composition Of A Climax Forest System In Boone County, Kentucky, Michael Held

Masters Theses & Specialist Projects

The structure and composition of a 52 hectare mature mesic hardwood forest in Boone County, Kentucky was studied during 1973-74. Acer saccharum was the dominant tree species of the entire forest, with Fraxinus americana as the subdominant. In the understory vegetation, Acer and Fraxinus were among the dominant genera, thus this forest system can be described as being at the climax stage of development. A previously cleared area in the forest was also analyzed. It was found that the dominant tree species of the canopy were also dominant in the genera of the understory in the disturbed area, indicating a …

Lethatlity Of And Elicitation Of Protective Antibody By Vibrio Parahaemolyticus Attc 17803, Richard Carlucci

Masters Theses & Specialist Projects

The LD₅₀ of Vibrio parahaemolyticus ATCC 17803 for 13- to 18-g male mice, strain ICR, as determined by the 50% endpoint method, was found to be an estimated 7 x 10⁷ CFU, when administered intraperitoneally in 0.5 ml of 24-h broth culture, O. D. 0.5 at 650 nm, diluted to 10^-0.72.

The elicitation of protective antibody by this bacterium in male New Zealand White rabbits was demonstrated by testing control, baseline, and hyperimmune rabbit sera for their protective effect against 2 LD₅₀ of the bacterium administered intraperitoneally in 13- to 18-g male mice, strain ICR. …

Effects Of Photoperiod, Temperature And Preconditioning On The Molt Cycle Of Samples From A Michigan Population Of Orconectes Immunis (Hagen), Robert Van Hoff

Masters Theses & Specialist Projects

Immature Orconectes immunis were obtained from Michigan in August, 1973, and July 1974 to determine the effects of photoperiod and temperature on the molt cycle of the crayfish and to test the effects of preconditioning on this species at a cold (7C) temperature from 45 to 90 days. Two sets of 3 experiments were conducted, each involving photoperiod, temperature and preconditioning. The first experiment of each set was initiated within a day after bringing the animals from the field, the subsequent two experiments of each set being initiated at 45-day intervals thereafter.

There were significant responses to photoperiod among the …

Ua66/6/3/1 Annual Newsletter, Kentucky Gamma, Alpha Epsilon Delta

Student Organizations

Newsletter created by and about the Kentucky Gamma Chapter of Alpha Epsilon Delta premedical honor society sponsored by WKU Biology.