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Full-Text Articles in Life Sciences
Autocrine Effects Of Catecholamines On Macrophage Release Of Interleukin-6 (Il-6), Shaunta D. Poe
Autocrine Effects Of Catecholamines On Macrophage Release Of Interleukin-6 (Il-6), Shaunta D. Poe
Theses and Dissertations
Effects of norepinephrine (NE) on macrophage cytokine release are complex because the cells have both α2 and β2 adrenergic receptors, which mediate opposing actions. Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. …
Novel Characteristics Of Murine Bone Marrow-Derived Macrophages And Human Macrophage-Like Cells, George Tharwat Georges
Novel Characteristics Of Murine Bone Marrow-Derived Macrophages And Human Macrophage-Like Cells, George Tharwat Georges
Theses and Dissertations
These studies provide evidence for novel properties of macrophages derived from bone marrow stem cells. In study 1, treatment of activated mouse bone marrow-derived macrophages (BMM) with either catecholamine synthesis inhibitors (α-methyl-para-tyrosine and fusaric acid) or the β2 adrenergic receptor antagonist ICI 118,551 demonstrated that BMM produce catecholamines. The catecholamines modulated macrophage cytokine production through autocrine actions on adrenergic receptors. In study II, undifferentiated human bone marrow cells were incubated in 30% mouse L929 fibroblast conditioned medium and generated adherent cells within three days. The cells were clearly identifiable as macrophages based on surface proteins and phagocytic activity but produced …