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Full-Text Articles in Life Sciences
Phylogenetic Analysis Of Serine Proteases From Russell's Viper (Daboia Russelli Siamensis) And Agkistrodon Piscivorus Leucostoma Venom, Pattadon Sukkapan, Ying Jia, Issarang Nuchprayoon, John C. Pérez
Phylogenetic Analysis Of Serine Proteases From Russell's Viper (Daboia Russelli Siamensis) And Agkistrodon Piscivorus Leucostoma Venom, Pattadon Sukkapan, Ying Jia, Issarang Nuchprayoon, John C. Pérez
Biology Faculty Publications and Presentations
Serine proteases are widely found in snake venoms. They have variety of functions including contributions to hemostasis. In this study, five serine proteases were cloned and characterized from two different cDNA libraries: factor V activator (RVV-V), alpha fibrinogenase (RVAF) and beta fibrinogenase (RVBF) from Russell's viper (Daboia russelli siamensis), and plasminogen activator (APL-PA) and protein C activator (APL-C) from Agkistrodon piscivorus leucostoma. The snake venom serine proteases were clustered in phylogenetic tree according to their functions. KA/KS values suggested that accelerated evolution has occurred in the mature protein coding regions in cDNAs of snake venom …
Molecular Cloning And Characterization Of Cdnas Encoding Metalloproteinases From Snake Venom Glands, Ying Jia, John C. Perez
Molecular Cloning And Characterization Of Cdnas Encoding Metalloproteinases From Snake Venom Glands, Ying Jia, John C. Perez
Biology Faculty Publications and Presentations
Snake venom metalloproteinases (SVMPs) are a superfamily of zinc-dependent proteases and participate in a number of important biological, physiological and pathophysiological processes. In this work, we simultaneously amplified 9 cDNAs encoding different classes of metalloproteinases from glands of four different snake species (Agkistrodon contortrix laticinctus, Crotalus atrox, Crotalus viridis viridis and Agkistrodon piscivorus leucostoma) by RT-PCR with a pair of primers. Among the encoded metalloproteinases, two enzymes (AclVMP-I and AplVMP-I), three enzymes (CaVMP-II, CvvVMP-II and AplVMP-II) and four enzymes (AclVMP-III, CaVMP-III, CvvVMP-III and AplVMP-III) …
Cdna Cloning, Expression And Fibrin(Ogen)Olytic Activity Of Two Low-Molecular Weight Snake Venom Metalloproteinases, Ying Jia, Sara E. Lucena, Esteban Cantu, Elda E. Sánchez, John C. Perez
Cdna Cloning, Expression And Fibrin(Ogen)Olytic Activity Of Two Low-Molecular Weight Snake Venom Metalloproteinases, Ying Jia, Sara E. Lucena, Esteban Cantu, Elda E. Sánchez, John C. Perez
Biology Faculty Publications and Presentations
Two cDNA clones, AplVMP1 and AplVMP2, were isolated from a snake (Agkistrodon piscivorus leucostoma) venom gland cDNA library. The full-length cDNA sequence of AplVMP1 with a calculated molecular mass of 46.61 kDa is 1,233 bp in length. AplVMP1 encodes PI class metalloproteinase with an open reading frame of 411 amino acid residues that includes signal peptide, pro-domain and metalloproteinase domains. The full-length cDNA of the AplVMP2 (1,371bp) has a calculated molecular mass of 51.16 kDa and encodes PII class metalloproteinase. The open reading frame of AplVMP2 with a 457 amino acid residues is composed of signal peptide, pro-domain, …
Complementary Dna Sequencing And Identification Of Mrnas From Venomous Gland Of Agkistrodon Piscivorus Leucostoma, Ying Jia, Bruno A. Cantu, Elda E. Sánchez, John C. Pérez
Complementary Dna Sequencing And Identification Of Mrnas From Venomous Gland Of Agkistrodon Piscivorus Leucostoma, Ying Jia, Bruno A. Cantu, Elda E. Sánchez, John C. Pérez
Biology Faculty Publications and Presentations
To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from venom gland of Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2,112 independent clones, we have obtained ESTs for 1,309 (62%) cDNAs which showed significant deduced amino acid sequence similarity (scores > 80) to previously characterized proteins in NCBI database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of < 80 with known GenBank accessions. The most highly expressed gene encoding phospholipase A2 (PLA2) accounting for 35% of A. p. leucostoma venom …