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Complementary Dna Sequencing And Identification Of Mrnas From Venomous Gland Of Agkistrodon Piscivorus Leucostoma, Ying Jia, Bruno A. Cantu, Elda E. Sánchez, John C. Pérez
Complementary Dna Sequencing And Identification Of Mrnas From Venomous Gland Of Agkistrodon Piscivorus Leucostoma, Ying Jia, Bruno A. Cantu, Elda E. Sánchez, John C. Pérez
Biology Faculty Publications and Presentations
To advance our knowledge on the snake venom composition and transcripts expressed in venom gland at the molecular level, we constructed a cDNA library from venom gland of Agkistrodon piscivorus leucostoma for the generation of expressed sequence tags (ESTs) database. From the randomly sequenced 2,112 independent clones, we have obtained ESTs for 1,309 (62%) cDNAs which showed significant deduced amino acid sequence similarity (scores > 80) to previously characterized proteins in NCBI database. Ribosomal proteins make up 47 clones (2%) and the remaining 756 (36%) cDNAs represent either unknown identity or show BLASTX sequence identity scores of < 80 with known GenBank accessions. The most highly expressed gene encoding phospholipase A2 (PLA2) accounting for 35% of A. p. leucostoma venom …